Detailed information of TA system TA061619

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): orf9-orf10/-
• Location: 91949..93333
• Replicon: plasmid unnamed
• Accession: NZ_CP014644
• Organism: Lysinibacillus sphaericus strain OT4b.25

Toxin (Protein)

• Gene name: ORF9
• Uniprot ID: -
• Locus tag: AR327_RS23110
• Protein ID: WP_012291646.1
• Coordinates: 91949..92494 (+)
• Length: 182 a.a.

Antitoxin (Protein)

• Gene name: ORF10
• Uniprot ID: B1I081
• Locus tag: AR327_RS23115
• Protein ID: WP_012291647.1
• Coordinates: 92494..93333 (+)
• Length: 280 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AR327_RS23080	87198..87953	+	756	WP_012291640.1	hypothetical protein	-
AR327_RS23085	88139..89236	+	1098	WP_158511885.1	hypothetical protein	-
AR327_RS23090	89240..90376	+	1137	WP_012291642.1	hypothetical protein	-
AR327_RS23095	90396..90662	+	267	WP_158511886.1	DUF1292 domain-containing protein	-
AR327_RS23100	90649..91200	+	552	WP_145912405.1	ATP-binding protein	-
AR327_RS23105	91240..91728	+	489	WP_012291645.1	hypothetical protein	-
AR327_RS23110	91949..92494	+	546	WP_012291646.1	NYN domain-containing protein	Toxin
AR327_RS23115	92494..93333	+	840	WP_012291647.1	hypothetical protein	Antitoxin
AR327_RS23120	93460..94311	+	852	WP_061504576.1	hypothetical protein	-
AR327_RS23125	94356..94631	+	276	WP_041659272.1	hypothetical protein	-
AR327_RS23130	94919..95287	+	369	WP_031417274.1	hypothetical protein	-
AR327_RS23135	95728..96771	+	1044	WP_012291651.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_010381 Lysinibacillus sphaericus C3-41 Non-Mobilizable plasmid TA010167 1..177642 0.006	inside	Non-Mobilizable plasmid	-	-	1..176083	176083

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• NYN domain (4-158)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 182 a.a.
• Molecular weight: 20304.38 Da
• Isoelectric Point: 9.4376

>T61619 WP_012291646.1 NZ_CP014644:91949-92494 [Lysinibacillus sphaericus]
MKKTAILVDEMNVIQQLRRLGIHGFNPWNAFYQALQRWNGDKKGVKAFYCANVPKEDAEHEKRNNFFEGLINHNIKVSVG
TVVYDSNSRRIAKGVDVALALDIVEHARSGVQDIFICTGDADVVPAIIRAQELGSRVHVVVSRAIPAKNITLAADSVVRL
EDILETMPPHRIKYFAKERVS

Nucleotide

• Length: 546 bp

>T61619 NZ_CP014644:91949-92494 [Lysinibacillus sphaericus]
ATGAAAAAGACAGCTATTTTAGTAGATGAAATGAACGTGATTCAACAACTCCGACGTTTAGGAATCCATGGATTCAATCC
CTGGAATGCATTTTATCAGGCGTTACAACGTTGGAATGGTGATAAGAAAGGTGTTAAAGCATTTTATTGTGCTAACGTAC
CTAAAGAAGATGCAGAACATGAAAAGCGTAATAATTTCTTTGAAGGATTAATAAATCATAACATCAAAGTATCAGTAGGT
ACTGTTGTATATGACTCAAACTCAAGACGGATAGCAAAAGGTGTAGATGTTGCCTTAGCGCTGGACATAGTAGAACATGC
TAGAAGCGGTGTACAAGATATTTTCATCTGTACAGGTGATGCTGATGTTGTGCCTGCCATTATTCGCGCACAGGAGCTCG
GTAGTCGTGTTCACGTTGTAGTTTCCAGGGCTATCCCAGCTAAAAATATCACCCTTGCAGCTGATTCAGTGGTTAGATTA
GAGGATATCTTAGAAACCATGCCTCCTCACCGCATTAAATACTTTGCTAAGGAGCGTGTGAGCTAA

Antitoxin

Protein

• Length: 280 a.a.
• Molecular weight: 31528.09 Da
• Isoelectric Point: 5.2827

>AT61619 WP_012291647.1 NZ_CP014644:92494-93333 [Lysinibacillus sphaericus]
METAPQEIFDLTLAKKTENEKELENAYRRIPDEYHDFIVLLEQKIKITDSRYFLAEEFITMGAYQDEKGIYLKIKKPYMT
VDGRIRMARDEHSKKEAKLIISEPQIIQVGNRICMNVTVESEIHGTATGTIEVNINGFGVDKKNPVANAQTSALGRALGF
LGYGIIGTAATASLDEATNLYNSLFASPSKEQVDPKKPEIKRVRICGPIEYKNNDLGYVQIQLETEEYVIMQIGKSLFRY
ENILVTNAVVKVRGWWNGEIFAAAPDQDIILDQNKKIVS

Nucleotide

• Length: 840 bp

>AT61619 NZ_CP014644:92494-93333 [Lysinibacillus sphaericus]
ATGGAGACAGCGCCACAAGAGATATTTGACCTTACACTTGCCAAAAAGACTGAAAATGAAAAGGAATTAGAGAATGCTTA
CAGAAGGATTCCGGATGAATACCATGACTTTATTGTATTGCTGGAGCAAAAAATTAAAATTACTGATAGTAGATACTTTT
TAGCAGAGGAATTTATAACAATGGGTGCCTACCAAGACGAAAAAGGTATCTATTTAAAGATTAAAAAGCCGTATATGACT
GTTGATGGCCGTATTCGAATGGCTCGCGATGAACATTCTAAAAAGGAAGCCAAACTAATTATTTCTGAACCTCAAATTAT
ACAAGTGGGAAATCGAATTTGTATGAATGTGACAGTTGAAAGTGAAATCCATGGCACTGCTACAGGAACTATAGAGGTTA
ATATTAACGGTTTTGGGGTAGATAAGAAGAATCCTGTAGCTAACGCACAAACTAGCGCTTTAGGACGTGCATTAGGCTTT
TTAGGGTACGGTATTATAGGTACTGCAGCAACCGCTTCATTAGATGAGGCTACAAACCTATATAACTCTTTATTTGCTTC
TCCAAGTAAGGAACAAGTTGATCCAAAGAAACCCGAGATTAAACGAGTTAGAATTTGTGGACCGATCGAATATAAAAACA
ACGACTTAGGGTATGTTCAAATACAGCTAGAAACTGAGGAATATGTAATAATGCAAATTGGAAAAAGCTTATTCAGATAT
GAAAATATACTGGTAACAAATGCAGTAGTAAAGGTGCGTGGTTGGTGGAACGGTGAAATATTCGCAGCTGCACCTGACCA
GGATATTATCTTGGACCAAAATAAAAAGATTGTTTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A6H0A0V0