10071

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type III Classification (family/domain) toxIN/-
Location 1473..2241 Replicon chromosome
Accession NZ_LDDO01000030
Organism Escherichia coli strain CFSAN026791

Toxin (Protein)


Gene name toxN Uniprot ID -
Locus tag WQ70_RS10970 Protein ID WP_000675353.1
Coordinates 1726..2241 (+) Length 172 a.a.

Antitoxin (RNA)


Gene name toxI
Locus tag -
Coordinates 1473..1683 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WQ70_RS10955 (WQ70_10965) 1..150 - 150 Protein_0 plasmid mobilization relaxosome protein MobC -
WQ70_RS10960 (WQ70_10970) 215..406 + 192 WP_000165987.1 Rop family plasmid primer RNA-binding protein -
- 1473..1683 + 211 - - Antitoxin
WQ70_RS10970 (WQ70_10980) 1726..2241 + 516 WP_000675353.1 type III toxin-antitoxin system ToxN/AbiQ family toxin Toxin
WQ70_RS10975 (WQ70_10985) 2353..3063 - 711 WP_024177388.1 hypothetical protein -
WQ70_RS10980 (WQ70_10990) 3608..3865 + 258 WP_001508827.1 hypothetical protein -
WQ70_RS10985 (WQ70_10995) 3875..4156 + 282 WP_024177387.1 hypothetical protein -
WQ70_RS30825 4469..4702 - 234 WP_029401705.1 MbeD family mobilization/exclusion protein -
WQ70_RS30830 4709..5227 - 519 WP_043991090.1 MbeB family mobilization protein -
WQ70_RS31985 5265..5696 - 432 Protein_9 relaxase/mobilization nuclease domain-containing protein -
WQ70_RS10995 (WQ70_11005) 5905..6141 - 237 Protein_10 MobC family plasmid mobilization relaxosome protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ToxN/AbiQ toxin

(1-160)


Sequences

Toxin        


Download         Length: 172 a.a.        Molecular weight: 19763.71 Da        Isoelectric Point: 5.6847

>T10071 WP_000675353.1 NZ_LDDO01000030:1726-2241 [Escherichia coli]
MKFYTISSSYIKYLKDFDDKVPNSEDPTYNNPKAFIGIVLEIEGHKYLAPLTSPKAWHANVKESSPAFFKLHENGVPDNQ
LGLINLKFMIPIIEAEVSLLDLDSMPDTPYKRMLYKQLQFIRVNEDKISEKSKLLRNLALQGRMQGTCDFAVLEEKYQHF
GKKPEDMEIDD

Download         Length: 516 bp

>T10071 NZ_LDDO01000030:1726-2241 [Escherichia coli]
ATGAAATTTTACACAATATCAAGCAGTTACATAAAATACCTGAAGGACTTTGATGACAAAGTTCCCAATAGCGAAGATCC
TACATACAACAATCCTAAGGCTTTCATTGGCATAGTACTAGAGATTGAAGGACATAAATATTTAGCACCTTTAACATCGC
CAAAAGCATGGCATGCTAATGTAAAAGAGTCATCACCCGCCTTCTTCAAACTTCATGAAAACGGTGTCCCTGATAATCAG
CTTGGATTGATTAATCTGAAATTTATGATTCCAATAATCGAAGCTGAAGTGTCTTTACTTGATCTGGATAGCATGCCTGA
TACGCCTTACAAAAGAATGCTATATAAACAGTTACAATTTATTCGTGTAAATGAAGATAAAATATCTGAAAAATCAAAAC
TATTAAGAAACCTTGCCTTGCAAGGCAGAATGCAAGGAACATGCGACTTTGCCGTGTTAGAAGAAAAATATCAACATTTC
GGTAAAAAACCTGAAGACATGGAAATAGACGATTAA

Antitoxin


Download         Length: 211 bp

>AT10071 NZ_LDDO01000030:1473-1683 [Escherichia coli]
AAGGTGATTTGCTACCTTTAAGTGCAGCTAGAAATTTAGGTGATTTGCTACCTTTAAGTGCAGCTAGAAATTTAGGTGAT
TTGCTACCTTTAAGTGCAGCTAGAAATTCAGGTGATTTGCTACCTTTAAGTGCAGCTAGAAATTCAGGTGATTTGCTACC
TTTAAGTGCAGCTAGAAATTTAGGTCATTTACTACCTTAAAGTAATAAAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T6317 Pectobacterium atrosepticum

82.143

98.246

0.807
T6366 Bacillus thuringiensis serovar kurstaki str. HD73

32.222

100

0.339
T6365 Lactococcus lactis

30.409

100

0.304

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Download structure file

References

(1) Chantal K Guegler et al. (2021) Shutoff of host transcription triggers a toxin-antitoxin system to cleave phage RNA and abort infection. Molecular Cell 81(11):2361-2373.e9. [PubMed:33838104] experimental


experimental experimental literature