Detailed information of TA system TA006365

Experimentally validated

Overview

TA module

• Type: III
• Classification (family/domain): abiQ-antiQ/-
• Location: 3504..4162
• Replicon: plasmid pSRQ900
• Accession: AF001314
• Organism: Lactococcus lactis

Toxin (Protein)

• Gene name: abiQ
• Uniprot ID: -
• Locus tag: abiQ
• Protein ID: AAC98713.1
• Coordinates: 3611..4162 (+)
• Length: 184 a.a.

Antitoxin (RNA)

Gene name	antiQ
Locus tag	antiQ
Coordinates	3504..3602 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Locus_0	271..1431	+	1161	AAC98710.1	RepB	-
Locus_1	1428..2015	+	588	AAC98711.1	unknown	-
Locus_2	1991..3181	+	1191	AAC98712.2	HsdS	-
-	3504..3602	+	99	-	-	Antitoxin
Locus_3	3611..4162	+	552	AAC98713.1	AbiQ	Toxin
Locus_4	4705..6234	+	1530	AAK57817.1	unknown	-
Locus_5	6581..6808	+	228	AAK57818.1	unknown	-
Locus_6	6810..7091	+	282	AAK57819.1	unknown	-
Locus_7	7190..7846	-	657	AAK57820.1	unknown	-
Locus_8	7885..8337	-	453	AAK57821.1	unknown	-
Locus_9	8464..8844	+	381	AAK57816.1	unknown	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..10836	10836

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ToxN/AbiQ toxin (14-176)

Sequences

Toxin

Protein

• Length: 184 a.a.
• Molecular weight: 21723.41 Da
• Isoelectric Point: 10.1403

>T6365 AAC98713.1 AF001314:3611-4162 [Lactococcus lactis]
MSSFFYKEILRMTLRFFTVTDEYIAYLRKFESKVHYQYENNASTYVGVVLKKNDFNYFIPLSSYKKGNPEKDKAMKKRSR
IVTRLFEIGNINNPLGYLLHHNMIPVPDSELIPLPLDLKKPKHKMMQKQLIYMKSISEKIENKSEVVYRKAAHEKDGYYL
KFSCDFKLLEAKATLYSKKSTFQ

Nucleotide

• Length: 552 bp

>T6365 AF001314:3611-4162 [Lactococcus lactis]
ATGAGTAGCTTTTTTTATAAGGAGATTTTACGAATGACGTTAAGATTCTTTACTGTAACTGATGAATATATAGCTTATTT
ACGTAAGTTTGAAAGTAAAGTTCATTATCAATATGAAAATAATGCCAGCACTTATGTAGGGGTTGTTTTGAAAAAGAATG
ATTTCAATTACTTTATACCTTTGTCAAGCTACAAAAAGGGGAACCCCGAGAAAGACAAAGCAATGAAAAAAAGAAGTCGA
ATAGTAACTAGACTTTTTGAAATTGGCAATATAAATAATCCTCTTGGATATTTATTACATCATAATATGATTCCAGTTCC
TGACAGCGAATTAATACCCCTACCTTTAGATCTTAAGAAACCTAAACATAAAATGATGCAAAAACAACTAATTTATATGA
AAAGTATCAGCGAAAAAATTGAAAATAAATCTGAAGTGGTATATAGAAAAGCTGCTCATGAAAAAGATGGATATTACTTA
AAATTTTCCTGTGATTTTAAACTACTAGAAGCTAAAGCAACATTATATTCTAAGAAATCTACATTTCAATAA

Antitoxin

Nucleotide

• Length: 99 bp

>AT6365 AF001314:3504-3602 [Lactococcus lactis]
TATCCAAGCCATAGTTGGAATTGATAAAATTGGAGTATCCAAGCCATAGTTGGAATTGATAAAATTGGAGTATCCAAGCC
ATAGTTGGAATTGATAAAA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T6366	Bacillus thuringiensis serovar kurstaki str. HD73	33.333	96.721	0.322
T10071	Escherichia coli strain CFSAN026791	30.409	100	0.304

Multiple sequence alignment

Structures

Antitoxin

References

(1) Julie E Samson et al. (2013) Structure and activity of AbiQ, a lactococcal endoribonuclease belonging to the type III toxin-antitoxin system. Molecular Microbiology 87(4):756-68. [PubMed:23279123]


 protein structure literature