Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yeeF-yezG/- |
| Location | 746677..749161 | Replicon | chromosome |
| Accession | NZ_CP034484 | ||
| Organism | Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10 strain NCIB 3610 | ||
Toxin (Protein)
| Gene name | yeeF | Uniprot ID | O31506 |
| Locus tag | EJJ34_RS03930 | Protein ID | WP_003242909.1 |
| Coordinates | 747152..749161 (-) | Length | 670 a.a. |
Antitoxin (Protein)
| Gene name | yezG | Uniprot ID | C0H3X4 |
| Locus tag | EJJ34_RS03925 | Protein ID | WP_010886434.1 |
| Coordinates | 746677..747132 (-) | Length | 152 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EJJ34_RS03905 (EJJ34_03905) | 742537..744336 | + | 1800 | WP_003233883.1 | ATP-dependent helicase | - |
| EJJ34_RS03910 (EJJ34_03910) | 744449..745597 | + | 1149 | WP_009966754.1 | GIY-YIG nuclease family protein | - |
| EJJ34_RS03915 (EJJ34_03915) | 745859..746164 | - | 306 | WP_003242885.1 | hypothetical protein | - |
| EJJ34_RS03920 (EJJ34_03920) | 746231..746437 | - | 207 | WP_003233877.1 | bacteriocin immunity protein | - |
| EJJ34_RS03925 (EJJ34_03925) | 746677..747132 | - | 456 | WP_010886434.1 | TIGR01741 family protein | Antitoxin |
| EJJ34_RS03930 (EJJ34_03930) | 747152..749161 | - | 2010 | WP_003242909.1 | LXG family T7SS effector deoxyribonuclease toxin YeeF | Toxin |
| EJJ34_RS03935 (EJJ34_03935) | 749373..750395 | + | 1023 | WP_003243884.1 | hypothetical protein | - |
| EJJ34_RS03940 (EJJ34_03940) | 750557..751687 | + | 1131 | WP_003243267.1 | response regulator aspartate phosphatase RapH | - |
| EJJ34_RS03945 (EJJ34_03945) | 751677..751850 | + | 174 | WP_003242557.1 | phosphatase RapH inhibitor PhrH | - |
| EJJ34_RS03950 (EJJ34_03950) | 752010..752729 | + | 720 | WP_003242611.1 | YebC/PmpR family DNA-binding transcriptional regulator | - |
| EJJ34_RS03955 (EJJ34_03955) | 752863..753300 | + | 438 | WP_003244206.1 | spore coat protein YeeK | - |
| EJJ34_RS03960 (EJJ34_03960) | 753415..753999 | + | 585 | WP_003243181.1 | TetR/AcrR family transcriptional regulator | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 670 a.a. Molecular weight: 98626.34 Da Isoelectric Point: 5.5500
>T10058 WP_003242909.1 NZ_CP034484:c749161-747152 [Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10]
MKVFEAKTLLSEATDRAKEYKELRTQMVNLRKALKGVADLSDSEFSGKGASNIKAFYHDHVGVADQWIDYIDMKIAFFNS
IAGAAEDKGLSDAYIEESFLEHELANANKKSKSIMSEQKKAMKDILNDIDDILPLDLFSTETFKDELADANDKRKKTLEK
LDALDEDLKTEYALSEPNEQFIKSDFQKLQEATGKGKNATPIHYNAKAYRESDIHKKKGDIEKRTEAYLKIKKEEAKERE
IEKLKERLKNYDYADADEFYEMAKTIGYENLTAEQQRYFTQIENTRELEAGFKGVAVGLYDSGKDAVVGLWDMVTDPGGT
VEAITGAMAHPIKTYEAISAAIEESYQKDMVNGDTYSRARWVSYAVGTVVTSIVGTKGVGAVSKTGTAAKVTTKVKTAAS
KSATAQKAITVSKQTVDHIKQKVNTGIEVSKKHVKTKLNQIGDLTLADILPYHPRHDLVPAGVPYNAVNGVTLKEGLQKF
AKVILPKPYGTSSSGRRTPAPHVPPVTVKYGEHFARWSRKKVLKPNIIYKTKEGYTYTTDNYGRITSVKADLQLGEAKRN
QYAQTNAGKPQDRKPDDDGGHLIATQFKGSGQFDNIVPMNSQINRSGGKWYEMEQEWAKALSKKPPKKVAVQIEPVYSGD
SLRPSYFDVTYKIGSRKEISVSIKNQPGG
MKVFEAKTLLSEATDRAKEYKELRTQMVNLRKALKGVADLSDSEFSGKGASNIKAFYHDHVGVADQWIDYIDMKIAFFNS
IAGAAEDKGLSDAYIEESFLEHELANANKKSKSIMSEQKKAMKDILNDIDDILPLDLFSTETFKDELADANDKRKKTLEK
LDALDEDLKTEYALSEPNEQFIKSDFQKLQEATGKGKNATPIHYNAKAYRESDIHKKKGDIEKRTEAYLKIKKEEAKERE
IEKLKERLKNYDYADADEFYEMAKTIGYENLTAEQQRYFTQIENTRELEAGFKGVAVGLYDSGKDAVVGLWDMVTDPGGT
VEAITGAMAHPIKTYEAISAAIEESYQKDMVNGDTYSRARWVSYAVGTVVTSIVGTKGVGAVSKTGTAAKVTTKVKTAAS
KSATAQKAITVSKQTVDHIKQKVNTGIEVSKKHVKTKLNQIGDLTLADILPYHPRHDLVPAGVPYNAVNGVTLKEGLQKF
AKVILPKPYGTSSSGRRTPAPHVPPVTVKYGEHFARWSRKKVLKPNIIYKTKEGYTYTTDNYGRITSVKADLQLGEAKRN
QYAQTNAGKPQDRKPDDDGGHLIATQFKGSGQFDNIVPMNSQINRSGGKWYEMEQEWAKALSKKPPKKVAVQIEPVYSGD
SLRPSYFDVTYKIGSRKEISVSIKNQPGG
Download Length: 2010 bp
>T10058 NZ_CP034484:c749161-747152 [Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10]
ATGAAAGTATTTGAAGCGAAAACACTGCTTTCGGAAGCGACAGACCGTGCAAAAGAGTACAAAGAACTAAGAACGCAAAT
GGTCAACTTGAGAAAAGCGTTAAAAGGAGTCGCTGATCTCAGTGACAGTGAATTCTCAGGAAAAGGCGCCAGCAACATCA
AGGCATTCTATCACGACCACGTCGGCGTCGCCGACCAGTGGATCGACTACATCGACATGAAAATCGCATTTTTCAACAGC
ATCGCCGGAGCCGCCGAAGACAAAGGCCTCTCAGACGCATACATCGAAGAATCCTTCCTGGAGCACGAACTGGCAAATGC
CAATAAAAAATCTAAATCCATCATGTCCGAACAGAAAAAAGCGATGAAAGACATCCTAAACGATATCGACGACATCCTCC
CGCTCGACCTGTTTTCAACAGAAACCTTCAAAGACGAGCTGGCCGATGCCAACGACAAACGCAAAAAAACACTGGAAAAA
TTGGACGCCTTGGACGAAGACCTGAAAACAGAATACGCCCTGTCTGAACCAAACGAACAATTCATCAAATCCGACTTCCA
AAAGCTCCAAGAAGCAACGGGTAAAGGCAAAAACGCCACACCCATCCACTACAACGCCAAGGCGTACCGTGAGAGTGACA
TACATAAGAAAAAAGGCGACATTGAAAAGCGGACTGAGGCTTACTTGAAGATTAAGAAAGAGGAAGCGAAGGAACGCGAG
ATTGAGAAACTGAAAGAAAGACTGAAAAACTACGATTATGCAGATGCCGATGAATTCTACGAAATGGCAAAAACGATCGG
CTATGAAAATCTGACAGCTGAACAACAGAGATACTTCACCCAAATTGAAAACACGCGTGAGCTTGAAGCAGGCTTCAAAG
GGGTTGCCGTCGGCCTTTATGATTCCGGGAAAGACGCGGTCGTAGGCCTGTGGGATATGGTCACAGATCCGGGAGGAACC
GTTGAAGCGATTACCGGAGCGATGGCTCACCCGATCAAAACGTATGAAGCCATTTCAGCGGCAATCGAAGAATCGTATCA
AAAAGACATGGTGAATGGTGACACCTACTCAAGAGCAAGATGGGTGTCATATGCGGTTGGCACTGTTGTCACATCAATTG
TCGGCACAAAAGGCGTAGGAGCCGTCTCAAAAACTGGCACAGCCGCCAAAGTGACGACCAAAGTCAAAACAGCAGCAAGC
AAATCAGCAACAGCCCAAAAAGCCATAACAGTCTCAAAACAAACCGTTGATCACATCAAACAAAAAGTAAACACAGGCAT
TGAAGTATCAAAAAAACACGTCAAAACCAAGTTAAATCAAATCGGAGATCTCACACTTGCGGATATTCTCCCGTATCATC
CCCGTCATGATTTAGTGCCGGCAGGCGTTCCTTACAACGCAGTCAACGGGGTCACGCTTAAAGAAGGACTGCAAAAATTC
GCCAAGGTCATCCTGCCGAAACCATACGGAACGTCATCAAGCGGAAGACGAACTCCAGCGCCACATGTACCGCCGGTAAC
CGTCAAATACGGCGAGCATTTCGCAAGATGGAGCAGAAAGAAAGTGCTGAAGCCTAATATCATCTACAAAACAAAAGAAG
GCTACACGTACACGACTGACAACTATGGTAGAATTACAAGTGTCAAAGCAGACTTGCAGCTGGGAGAAGCGAAACGAAAC
CAATATGCACAAACGAATGCTGGAAAGCCCCAGGACCGAAAGCCTGACGATGACGGCGGCCATTTAATCGCCACCCAATT
CAAAGGCTCAGGCCAGTTTGACAATATCGTGCCGATGAATTCTCAGATTAATAGGAGCGGTGGGAAATGGTATGAGATGG
AGCAGGAGTGGGCAAAAGCCCTAAGCAAAAAACCACCTAAAAAAGTAGCAGTTCAAATAGAACCAGTCTATTCTGGGGAT
TCACTTAGACCATCATATTTCGATGTAACCTACAAAATTGGGAGCAGAAAAGAAATAAGCGTCTCTATTAAAAATCAGCC
TGGGGGTTAG
ATGAAAGTATTTGAAGCGAAAACACTGCTTTCGGAAGCGACAGACCGTGCAAAAGAGTACAAAGAACTAAGAACGCAAAT
GGTCAACTTGAGAAAAGCGTTAAAAGGAGTCGCTGATCTCAGTGACAGTGAATTCTCAGGAAAAGGCGCCAGCAACATCA
AGGCATTCTATCACGACCACGTCGGCGTCGCCGACCAGTGGATCGACTACATCGACATGAAAATCGCATTTTTCAACAGC
ATCGCCGGAGCCGCCGAAGACAAAGGCCTCTCAGACGCATACATCGAAGAATCCTTCCTGGAGCACGAACTGGCAAATGC
CAATAAAAAATCTAAATCCATCATGTCCGAACAGAAAAAAGCGATGAAAGACATCCTAAACGATATCGACGACATCCTCC
CGCTCGACCTGTTTTCAACAGAAACCTTCAAAGACGAGCTGGCCGATGCCAACGACAAACGCAAAAAAACACTGGAAAAA
TTGGACGCCTTGGACGAAGACCTGAAAACAGAATACGCCCTGTCTGAACCAAACGAACAATTCATCAAATCCGACTTCCA
AAAGCTCCAAGAAGCAACGGGTAAAGGCAAAAACGCCACACCCATCCACTACAACGCCAAGGCGTACCGTGAGAGTGACA
TACATAAGAAAAAAGGCGACATTGAAAAGCGGACTGAGGCTTACTTGAAGATTAAGAAAGAGGAAGCGAAGGAACGCGAG
ATTGAGAAACTGAAAGAAAGACTGAAAAACTACGATTATGCAGATGCCGATGAATTCTACGAAATGGCAAAAACGATCGG
CTATGAAAATCTGACAGCTGAACAACAGAGATACTTCACCCAAATTGAAAACACGCGTGAGCTTGAAGCAGGCTTCAAAG
GGGTTGCCGTCGGCCTTTATGATTCCGGGAAAGACGCGGTCGTAGGCCTGTGGGATATGGTCACAGATCCGGGAGGAACC
GTTGAAGCGATTACCGGAGCGATGGCTCACCCGATCAAAACGTATGAAGCCATTTCAGCGGCAATCGAAGAATCGTATCA
AAAAGACATGGTGAATGGTGACACCTACTCAAGAGCAAGATGGGTGTCATATGCGGTTGGCACTGTTGTCACATCAATTG
TCGGCACAAAAGGCGTAGGAGCCGTCTCAAAAACTGGCACAGCCGCCAAAGTGACGACCAAAGTCAAAACAGCAGCAAGC
AAATCAGCAACAGCCCAAAAAGCCATAACAGTCTCAAAACAAACCGTTGATCACATCAAACAAAAAGTAAACACAGGCAT
TGAAGTATCAAAAAAACACGTCAAAACCAAGTTAAATCAAATCGGAGATCTCACACTTGCGGATATTCTCCCGTATCATC
CCCGTCATGATTTAGTGCCGGCAGGCGTTCCTTACAACGCAGTCAACGGGGTCACGCTTAAAGAAGGACTGCAAAAATTC
GCCAAGGTCATCCTGCCGAAACCATACGGAACGTCATCAAGCGGAAGACGAACTCCAGCGCCACATGTACCGCCGGTAAC
CGTCAAATACGGCGAGCATTTCGCAAGATGGAGCAGAAAGAAAGTGCTGAAGCCTAATATCATCTACAAAACAAAAGAAG
GCTACACGTACACGACTGACAACTATGGTAGAATTACAAGTGTCAAAGCAGACTTGCAGCTGGGAGAAGCGAAACGAAAC
CAATATGCACAAACGAATGCTGGAAAGCCCCAGGACCGAAAGCCTGACGATGACGGCGGCCATTTAATCGCCACCCAATT
CAAAGGCTCAGGCCAGTTTGACAATATCGTGCCGATGAATTCTCAGATTAATAGGAGCGGTGGGAAATGGTATGAGATGG
AGCAGGAGTGGGCAAAAGCCCTAAGCAAAAAACCACCTAAAAAAGTAGCAGTTCAAATAGAACCAGTCTATTCTGGGGAT
TCACTTAGACCATCATATTTCGATGTAACCTACAAAATTGGGAGCAGAAAAGAAATAAGCGTCTCTATTAAAAATCAGCC
TGGGGGTTAG
Antitoxin
Download Length: 152 a.a. Molecular weight: 22388.86 Da Isoelectric Point: 5.5500
>AT10058 WP_010886434.1 NZ_CP034484:c747132-746677 [Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10]
MQDLYQLIGEKLNDIIPGEWTKIYLYAEVLDDSTMVLFHFRTPENNQIIYSQDIPSHYNVSKDIFKTLLRELRELFEELR
TEHRNNNDDVWTNLTLTLDRSGEFQLDYNYDDILASELDGYERIAIWEYKNLGILPEDEDDKEFVISYLGL
MQDLYQLIGEKLNDIIPGEWTKIYLYAEVLDDSTMVLFHFRTPENNQIIYSQDIPSHYNVSKDIFKTLLRELRELFEELR
TEHRNNNDDVWTNLTLTLDRSGEFQLDYNYDDILASELDGYERIAIWEYKNLGILPEDEDDKEFVISYLGL
Download Length: 456 bp
>AT10058 NZ_CP034484:c747132-746677 [Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10]
ATGCAAGATTTGTATCAACTTATTGGAGAAAAACTCAATGACATCATACCTGGTGAATGGACAAAGATATATCTTTATGC
AGAAGTATTAGATGATTCCACAATGGTTCTTTTTCATTTCAGAACACCAGAAAATAATCAAATTATTTATTCACAAGATA
TACCTAGCCATTACAACGTAAGCAAGGATATTTTCAAGACGTTGTTAAGAGAACTAAGAGAACTTTTTGAAGAATTAAGA
ACTGAACACAGAAATAATAACGATGATGTTTGGACTAACCTTACACTAACATTGGATCGTAGCGGAGAATTCCAATTAGA
TTATAATTATGATGATATTTTAGCCTCAGAATTAGATGGATATGAAAGAATCGCAATATGGGAATATAAAAACCTTGGTA
TTTTGCCTGAAGATGAGGATGATAAAGAATTTGTAATTAGTTACCTCGGTCTATAA
ATGCAAGATTTGTATCAACTTATTGGAGAAAAACTCAATGACATCATACCTGGTGAATGGACAAAGATATATCTTTATGC
AGAAGTATTAGATGATTCCACAATGGTTCTTTTTCATTTCAGAACACCAGAAAATAATCAAATTATTTATTCACAAGATA
TACCTAGCCATTACAACGTAAGCAAGGATATTTTCAAGACGTTGTTAAGAGAACTAAGAGAACTTTTTGAAGAATTAAGA
ACTGAACACAGAAATAATAACGATGATGTTTGGACTAACCTTACACTAACATTGGATCGTAGCGGAGAATTCCAATTAGA
TTATAATTATGATGATATTTTAGCCTCAGAATTAGATGGATATGAAAGAATCGCAATATGGGAATATAAAAACCTTGGTA
TTTTGCCTGAAGATGAGGATGATAAAGAATTTGTAATTAGTTACCTCGGTCTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10061 | Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10 strain NCIB 3610 |
61.02 |
85.814 |
0.524 |
| T10062 | Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10 strain NCIB 3610 |
59.295 |
85.167 |
0.505 |
| T10059 | Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10 strain NCIB 3610 |
65.6 |
47.081 |
0.309 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Kazuo Kobayashi (2021) Diverse LXG toxin and antitoxin systems specifically mediate intraspecies competition in Bacillus subtilis biofilms. PLoS Genetics 17(7):e1009682. [PubMed:34280190]