MGE detailed information
MGE type: Conjugative plasmid MGE length: 131479 bp
Accession: NZ_CP103658 Location: 1..131479 Organism: Escherichia coli strain 3150 Replicon: plasmid pMB5646_1
Accession: NZ_CP103658 Location: 1..131479 Organism: Escherichia coli strain 3150 Replicon: plasmid pMB5646_1
Gene structure
The 5 kb flanking regions of this MGE are displayed.
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| M5R25_RS24660 (1) | 1..978 | + | 978 | WP_000361611.1 | RepB family plasmid replication initiator protein | - |
| M5R25_RS24665 (1263) | 1263..2003 | - | 741 | WP_001066953.1 | tyrosine-type recombinase/integrase | - |
| M5R25_RS24670 (2124) | 2124..2306 | - | 183 | WP_042344623.1 | hypothetical protein | - |
| M5R25_RS24675 (2373) | 2373..2662 | - | 290 | Protein_3 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| M5R25_RS24680 (2649) | 2649..2917 | - | 269 | Protein_4 | type II toxin-antitoxin system ParD family antitoxin | - |
| M5R25_RS24685 (3449) | 3449..4618 | + | 1170 | WP_001072355.1 | hypothetical protein | - |
| M5R25_RS24690 (4651) | 4651..4779 | + | 129 | Protein_6 | IS481 family transposase | - |
| M5R25_RS24695 (4904) | 4904..5107 | - | 204 | Protein_7 | hypothetical protein | - |
| M5R25_RS24700 (5213) | 5213..5488 | - | 276 | WP_000421272.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin Detail |
| M5R25_RS24705 (5488) | 5488..5772 | - | 285 | WP_001387467.1 | ribbon-helix-helix domain-containing protein | Antitoxin Detail |
| M5R25_RS24710 (6377) | 6377..7129 | + | 753 | WP_000562172.1 | IclR family transcriptional regulator | - |
| M5R25_RS24715 (7175) | 7175..8140 | - | 966 | WP_001022265.1 | 2-keto-3-deoxygluconate transporter | - |
| M5R25_RS24720 (8173) | 8173..8553 | - | 381 | WP_024946717.1 | Rid family detoxifying hydrolase | - |
| M5R25_RS24725 (8578) | 8578..9468 | - | 891 | WP_001077068.1 | dihydrodipicolinate synthase family protein | - |
| M5R25_RS24730 (9701) | 9701..9895 | - | 195 | WP_000796505.1 | hypothetical protein | - |
| M5R25_RS24735 (9869) | 9869..10309 | + | 441 | Protein_15 | zinc-binding dehydrogenase | - |
| M5R25_RS24740 (10440) | 10440..11318 | + | 879 | WP_000338039.1 | sugar ABC transporter permease | - |
| M5R25_RS24745 (11308) | 11308..12195 | + | 888 | WP_001271561.1 | carbohydrate ABC transporter permease | - |
| M5R25_RS24750 (12206) | 12206..13030 | + | 825 | WP_000922702.1 | phosphodiesterase | - |
| M5R25_RS24755 (13036) | 13036..14109 | + | 1074 | WP_000950177.1 | sn-glycerol-3-phosphate import ATP-binding protein UgpC | VF |
| M5R25_RS24760 (14102) | 14102..15412 | + | 1311 | WP_000476108.1 | ABC transporter substrate-binding protein | - |
| M5R25_RS24765 (15471) | 15471..15779 | - | 309 | Protein_21 | IS4 family transposase ISEc13 | Transposase |
| M5R25_RS24770 (15856) | 15856..16029 | - | 174 | WP_000338295.1 | Tn3 family transposase | - |
| M5R25_RS24775 (16111) | 16111..17307 | - | 1197 | Protein_23 | IS3 family transposase | - |
| M5R25_RS24780 (17332) | 17332..18036 | + | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| M5R25_RS24785 (18090) | 18090..21677 | + | 3588 | Protein_25 | Multifunctional conjugation protein TraI | Relaxase |
| M5R25_RS24790 (21697) | 21697..22443 | + | 747 | WP_000205718.1 | conjugal transfer pilus acetylase TraX | - |
| M5R25_RS24795 (22498) | 22498..23058 | + | 561 | WP_000139341.1 | fertility inhibition protein FinO | - |
| M5R25_RS24800 (23189) | 23189..23401 | + | 213 | WP_013023861.1 | hypothetical protein | - |
| M5R25_RS24805 (23914) | 23914..24200 | + | 287 | Protein_29 | DUF2726 domain-containing protein | - |
| - (24167) | 24167..24227 | - | 61 | NuclAT_2 | - | - |
| - (24167) | 24167..24227 | - | 61 | NuclAT_2 | - | - |
| - (24167) | 24167..24227 | - | 61 | NuclAT_2 | - | - |
| - (24167) | 24167..24227 | - | 61 | NuclAT_2 | - | - |
| M5R25_RS24810 (24272) | 24272..24406 | + | 135 | WP_023144756.1 | Hok/Gef family protein | - |
| M5R25_RS24815 (24703) | 24703..24813 | + | 111 | Protein_31 | replication protein | - |
| M5R25_RS24820 (24882) | 24882..25664 | - | 783 | WP_001300609.1 | IS21 family transposase IS100kyp | Transposase |
| M5R25_RS24825 (25661) | 25661..26683 | - | 1023 | WP_000255944.1 | IS21 family transposase IS100kyp | Transposase |
| M5R25_RS24830 (26770) | 26770..26916 | + | 147 | Protein_34 | replication protein A | - |
| M5R25_RS24835 (27022) | 27022..27153 | + | 132 | Protein_35 | protein CopA/IncA | - |
| M5R25_RS24840 (27153) | 27153..27227 | + | 75 | WP_031943482.1 | RepA leader peptide Tap | - |
| M5R25_RS24845 (27220) | 27220..28077 | + | 858 | WP_000130640.1 | incFII family plasmid replication initiator RepA | - |
| M5R25_RS24850 (29017) | 29017..29670 | + | 654 | WP_000616807.1 | CPBP family intramembrane metalloprotease | - |
| M5R25_RS24855 (29763) | 29763..30020 | + | 258 | WP_000557619.1 | type II toxin-antitoxin system antitoxin PemI | - |
| M5R25_RS24860 (29953) | 29953..30354 | + | 402 | WP_001398199.1 | type II toxin-antitoxin system toxin endoribonuclease PemK | - |
| M5R25_RS24865 (30603) | 30603..30695 | + | 93 | Protein_41 | IS1 family transposase | - |
| M5R25_RS24870 (30752) | 30752..31456 | + | 705 | WP_001067858.1 | IS6 family transposase IS15DI | Transposase |
| M5R25_RS24875 (31578) | 31578..32282 | + | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| M5R25_RS24880 (32336) | 32336..32581 | - | 246 | Protein_44 | NAD(+)--rifampin ADP-ribosyltransferase | - |
| M5R25_RS24885 (32666) | 32666..33298 | - | 633 | WP_000186237.1 | Chloramphenicol acetyltransferase | VF |
| M5R25_RS24890 (33436) | 33436..34266 | - | 831 | WP_001334766.1 | Beta-lactamase OXA-1 | ARG |
| M5R25_RS24895 (34397) | 34397..34951 | - | 555 | WP_063840321.1 | Aminoglycoside N(6')-acetyltransferase type 1 | ARG |
| M5R25_RS24900 (35095) | 35095..35799 | - | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| M5R25_RS24905 (35864) | 35864..36544 | - | 681 | Protein_49 | IS91 family transposase ISAzo26 | Transposase |
| M5R25_RS24910 (36702) | 36702..37175 | + | 474 | WP_001389366.1 | trimethoprim-resistant dihydrofolate reductase DfrA17 | - |
| M5R25_RS24915 (37306) | 37306..38094 | + | 789 | WP_000503573.1 | Streptomycin 3''-adenylyltransferase | ARG |
| M5R25_RS24920 (38300) | 38300..38647 | + | 348 | WP_000679427.1 | Multidrug transporter EmrE | ARG |
| M5R25_RS24925 (38641) | 38641..39480 | + | 840 | WP_000259031.1 | Dihydropteroate synthase | ARG |
| M5R25_RS24930 (39608) | 39608..39811 | + | 204 | WP_000376616.1 | hypothetical protein | - |
| M5R25_RS24935 (39967) | 39967..41172 | + | 1206 | WP_000184001.1 | chromate efflux transporter | - |
| M5R25_RS24940 (41183) | 41183..41488 | + | 306 | WP_000130000.1 | hypothetical protein | Relaxase |
| M5R25_RS24945 (41532) | 41532..41597 | + | 66 | Protein_57 | EAL domain-containing protein | - |
| M5R25_RS24950 (41715) | 41715..42479 | + | 765 | WP_001389365.1 | IS6 family transposase IS6100 | Transposase |
| M5R25_RS24955 (42972) | 42972..43556 | - | 585 | WP_001137892.1 | macrolide-binding transcriptional repressor MphR(A) | - |
| M5R25_RS24960 (43556) | 43556..44794 | - | 1239 | WP_000004159.1 | macrolide resistance MFS transporter Mrx(A) | - |
| M5R25_RS24965 (44791) | 44791..45705 | - | 915 | WP_023063803.1 | hypothetical protein | ARG |
| M5R25_RS24970 (45827) | 45827..46531 | - | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| M5R25_RS24975 (46666) | 46666..46761 | + | 96 | WP_013362819.1 | 23S rRNA methyltransferase attenuation leader peptide | - |
| M5R25_RS24980 (46887) | 46887..47624 | + | 738 | WP_013362818.1 | rRNA adenine N-6-methyltransferase | ARG |
| M5R25_RS24985 (48254) | 48254..48703 | + | 450 | WP_013362817.1 | hypothetical protein | - |
| M5R25_RS24990 (48664) | 48664..49005 | + | 342 | WP_023909256.1 | 60 kDa chaperonin 2 | VF |
| M5R25_RS24995 (49280) | 49280..50764 | + | 1485 | WP_032152108.1 | IS91 family transposase ISVsa3 | Transposase |
| M5R25_RS25000 (51087) | 51087..51446 | - | 360 | Protein_68 | IS6 family transposase IS26 | Transposase |
| M5R25_RS25005 (51498) | 51498..52195 | + | 698 | WP_095033700.1 | IS1 family transposase IS1R | Transposase |
| M5R25_RS25010 (52210) | 52210..52518 | + | 309 | Protein_70 | amino acid-binding protein | - |
| M5R25_RS25015 (52526) | 52526..53212 | + | 687 | WP_001284954.1 | winged helix-turn-helix domain-containing protein | - |
| M5R25_RS25020 (53190) | 53190..53813 | - | 624 | WP_000088605.1 | Tetracycline repressor protein class B from transposon Tn10 | VF |
| M5R25_RS25025 (53895) | 53895..55100 | + | 1206 | WP_001089068.1 | Tetracycline resistance protein, class B | ARG |
| M5R25_RS25030 (55213) | 55213..55806 | - | 594 | WP_000428546.1 | tetracyline resistance-associated transcriptional repressor TetC | - |
| M5R25_RS25040 (56862) | 56862..56975 | + | 114 | Protein_76 | IS6 family transposase IS15DI | Transposase |
| M5R25_RS25045 (57113) | 57113..57283 | + | 171 | Protein_77 | DsbA family protein | - |
| M5R25_RS25050 (57273) | 57273..57779 | + | 507 | WP_000949452.1 | protein disulfide oxidoreductase | - |
| M5R25_RS25055 (58016) | 58016..58777 | + | 762 | WP_001225378.1 | Na(+)-translocating NADH-quinone reductase subunit C | - |
| M5R25_RS25060 (59124) | 59124..61010 | + | 1887 | WP_000118029.1 | FTR1 family protein | - |
| M5R25_RS25065 (61051) | 61051..61578 | + | 528 | WP_000178050.1 | iron transporter | - |
| M5R25_RS25070 (61682) | 61682..63061 | + | 1380 | WP_000119836.1 | Fe-S-containing protein | - |
| M5R25_RS25075 (63064) | 63064..64347 | + | 1284 | WP_000964653.1 | ABC transporter permease | - |
| M5R25_RS25080 (64337) | 64337..65467 | + | 1131 | WP_000729220.1 | ABC transporter permease | - |
| M5R25_RS25085 (65472) | 65472..66167 | + | 696 | WP_000117262.1 | Lipoprotein-releasing system ATP-binding protein LolD | VF |
| M5R25_RS25090 (66154) | 66154..66639 | + | 486 | WP_001267177.1 | TlpA disulfide reductase family protein | - |
| M5R25_RS25095 (66664) | 66664..67149 | + | 486 | WP_000874189.1 | hypothetical protein | - |
| M5R25_RS25100 (67271) | 67271..67801 | + | 531 | Protein_88 | histidine-type phosphatase | - |
| M5R25_RS25105 (67859) | 67859..68563 | - | 705 | WP_001067855.1 | IS6 family transposase IS26 | Transposase |
| M5R25_RS25110 (68631) | 68631..70316 | - | 1686 | Protein_90 | Multifunctional conjugation protein TraI | Relaxase |
| M5R25_RS25115 (70316) | 70316..72505 | - | 2190 | WP_024946705.1 | type IV conjugative transfer system coupling protein TraD | - |
| M5R25_RS25120 (72502) | 72502..73281 | - | 780 | WP_000199914.1 | hypothetical protein | - |
| M5R25_RS25125 (73484) | 73484..74215 | - | 732 | WP_000850429.1 | conjugal transfer complement resistance protein TraT | - |
| M5R25_RS25130 (74247) | 74247..74744 | - | 498 | WP_000605862.1 | entry exclusion protein | - |
| M5R25_RS25135 (74763) | 74763..77582 | - | 2820 | WP_001007062.1 | conjugal transfer mating-pair stabilization protein TraG | - |
| M5R25_RS25140 (77579) | 77579..78868 | - | 1290 | WP_259430694.1 | conjugal transfer protein TraH | - |
| M5R25_RS25145 (78855) | 78855..79247 | - | 393 | WP_000659962.1 | F-type conjugal transfer protein TrbF | - |
| M5R25_RS25150 (79228) | 79228..79575 | - | 348 | WP_071571857.1 | P-type conjugative transfer protein TrbJ | - |
| M5R25_RS25155 (79505) | 79505..80050 | - | 546 | WP_000059832.1 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | - |
| M5R25_RS25160 (80037) | 80037..80321 | - | 285 | WP_001617873.1 | type-F conjugative transfer system pilin chaperone TraQ | - |
| M5R25_RS25165 (80448) | 80448..80768 | - | 321 | WP_001348757.1 | conjugal transfer protein TrbA | - |
| M5R25_RS25175 (81628) | 81628..82230 | + | 603 | WP_000243712.1 | hypothetical protein | VF |
| M5R25_RS25180 (82527) | 82527..83348 | - | 822 | WP_001234469.1 | DUF932 domain-containing protein | - |
| M5R25_RS25185 (83467) | 83467..83754 | - | 288 | WP_000107535.1 | hypothetical protein | - |
| M5R25_RS25190 (83779) | 83779..83985 | - | 207 | WP_000275859.1 | hypothetical protein | - |
| M5R25_RS25195 (84055) | 84055..84228 | + | 174 | Protein_107 | hypothetical protein | - |
| M5R25_RS25200 (84226) | 84226..84456 | - | 231 | WP_001426396.1 | hypothetical protein | - |
| M5R25_RS25205 (84676) | 84676..84801 | - | 126 | WP_001372321.1 | type I toxin-antitoxin system Hok family toxin | Toxin Detail |
| M5R25_RS25210 (84743) | 84743..84892 | - | 150 | Protein_110 | plasmid maintenance protein Mok | - |
| - (84878) | 84878..85005 | - | 128 | NuclAT_0 | - | Antitoxin Detail |
| - (84878) | 84878..85005 | - | 128 | NuclAT_0 | - | Antitoxin Detail |
| - (84878) | 84878..85005 | - | 128 | NuclAT_0 | - | Antitoxin Detail |
| - (84878) | 84878..85005 | - | 128 | NuclAT_0 | - | Antitoxin Detail |
| - (86447) | 86447..86549 | - | 103 | NuclAT_1 | - | - |
| - (86447) | 86447..86549 | - | 103 | NuclAT_1 | - | - |
| - (86447) | 86447..86549 | - | 103 | NuclAT_1 | - | - |
| - (86447) | 86447..86549 | - | 103 | NuclAT_1 | - | - |
| M5R25_RS25220 (86518) | 86518..87280 | - | 763 | Protein_112 | plasmid SOS inhibition protein A | - |
| M5R25_RS25225 (87277) | 87277..87711 | - | 435 | WP_000845940.1 | conjugation system SOS inhibitor PsiB | - |
| M5R25_RS25230 (87766) | 87766..89724 | - | 1959 | WP_000117179.1 | ParB/RepB/Spo0J family partition protein | - |
| M5R25_RS25235 (89790) | 89790..90023 | - | 234 | WP_000005990.1 | DUF905 family protein | - |
| M5R25_RS25240 (90086) | 90086..90583 | - | 498 | WP_042045560.1 | single-stranded DNA-binding protein | - |
| M5R25_RS25245 (90733) | 90733..92634 | - | 1902 | WP_000936285.1 | group II intron reverse transcriptase/maturase | - |
| M5R25_RS25250 (93312) | 93312..93518 | - | 207 | WP_000547975.1 | hypothetical protein | - |
| M5R25_RS25255 (93431) | 93431..93766 | - | 336 | WP_013023883.1 | hypothetical protein | - |
| M5R25_RS25260 (93759) | 93759..94022 | - | 264 | WP_071781663.1 | hypothetical protein | - |
| M5R25_RS25265 (93929) | 93929..94147 | - | 219 | WP_001496291.1 | hypothetical protein | - |
| M5R25_RS25270 (94173) | 94173..94736 | - | 564 | WP_001348621.1 | class I SAM-dependent methyltransferase | - |
| M5R25_RS25275 (94784) | 94784..96145 | - | 1362 | WP_000170714.1 | DUF3560 domain-containing protein | - |
| M5R25_RS25280 (96197) | 96197..96427 | - | 231 | WP_000218642.1 | hypothetical protein | - |
| M5R25_RS25285 (97462) | 97462..97653 | - | 192 | WP_001027516.1 | hypothetical protein | - |
| M5R25_RS25290 (97650) | 97650..98072 | - | 423 | WP_000271753.1 | DUF1380 family protein | - |
| M5R25_RS25295 (98119) | 98119..98544 | - | 426 | WP_001198928.1 | antirestriction protein | - |
| M5R25_RS25300 (98961) | 98961..99737 | - | 777 | WP_001753849.1 | hypothetical protein | - |
| M5R25_RS25305 (99788) | 99788..100306 | - | 519 | WP_123325260.1 | DUF1380 family protein | - |
| M5R25_RS25310 (100236) | 100236..100457 | - | 222 | WP_001104873.1 | hypothetical protein | - |
| M5R25_RS25315 (100458) | 100458..101141 | - | 684 | WP_000086185.1 | DNA methylase | - |
| M5R25_RS25320 (101218) | 101218..101529 | - | 312 | WP_011254640.1 | hypothetical protein | - |
| M5R25_RS25325 (101526) | 101526..102428 | - | 903 | WP_001348615.1 | DUF1281 domain-containing protein | - |
| M5R25_RS25330 (103295) | 103295..104266 | - | 972 | WP_000817036.1 | ParB/RepB/Spo0J family plasmid partition protein | - |
| M5R25_RS25335 (104266) | 104266..105432 | - | 1167 | WP_000772446.1 | Sporulation initiation inhibitor protein Soj | VF |
| M5R25_RS25340 (106020) | 106020..106775 | - | 756 | WP_024946706.1 | replication initiation protein RepE | - |
| M5R25_RS25345 (107549) | 107549..108355 | - | 807 | WP_000016982.1 | site-specific integrase | - |
| M5R25_RS25350 (108356) | 108356..108661 | - | 306 | WP_001159868.1 | type II toxin-antitoxin system toxin CcdB | Toxin Detail |
| M5R25_RS25355 (108663) | 108663..108881 | - | 219 | WP_000813634.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin Detail |
| M5R25_RS25360 (109448) | 109448..109960 | + | 513 | WP_000151784.1 | hypothetical protein | - |
| M5R25_RS25365 (109994) | 109994..111127 | - | 1134 | WP_001542067.1 | DUF3800 domain-containing protein | - |
| M5R25_RS25370 (111294) | 111294..112067 | - | 774 | WP_000905949.1 | hypothetical protein | - |
| M5R25_RS25375 (112080) | 112080..112580 | - | 501 | WP_000528931.1 | HEPN family nuclease | - |
| M5R25_RS25385 (113722) | 113722..114636 | + | 915 | WP_000949004.1 | Putative metal ABC transporter substrate-binding protein Hpf | VF |
| M5R25_RS25390 (114636) | 114636..115463 | + | 828 | WP_000983710.1 | Iron(3+)-hydroxamate import ATP-binding protein FhuC | VF |
| M5R25_RS25395 (115460) | 115460..116317 | + | 858 | WP_001101723.1 | Manganese transport system membrane protein MntB | ARG |
| M5R25_RS25400 (116314) | 116314..117171 | + | 858 | WP_000968139.1 | Manganese transport system membrane protein MntB | ARG |
| M5R25_RS25405 (117405) | 117405..117662 | - | 258 | Protein_149 | transposase | - |
| M5R25_RS25410 (117660) | 117660..118073 | + | 414 | Protein_150 | phosphopyruvate hydratase | - |
| M5R25_RS25415 (118414) | 118414..118794 | + | 381 | WP_001318207.1 | fluoride efflux transporter CrcB | - |
| M5R25_RS25420 (119174) | 119174..120367 | - | 1194 | WP_000095526.1 | MFS transporter | - |
| M5R25_RS25425 (120503) | 120503..122227 | + | 1725 | WP_000602863.1 | N(2)-citryl-N(6)-acetyl-N(6)-hydroxylysine synthase | VF |
| M5R25_RS25430 (122228) | 122228..123175 | + | 948 | WP_000011908.1 | N(6)-hydroxylysine O-acetyltransferase | VF |
| M5R25_RS25435 (123175) | 123175..124917 | + | 1743 | WP_001015721.1 | Aerobactin synthase | VF |
| M5R25_RS25440 (124914) | 124914..126247 | + | 1334 | Protein_156 | L-lysine N6-monooxygenase | VF |
| M5R25_RS25445 (126273) | 126273..128474 | + | 2202 | WP_000973519.1 | Ferric aerobactin receptor | VF |
| M5R25_RS25450 (128854) | 128854..129551 | + | 698 | WP_223155668.1 | IS1 family transposase IS1S | Transposase |
| M5R25_RS25455 (129731) | 129731..130244 | - | 514 | Protein_159 | DUF4113 domain-containing protein | - |
| M5R25_RS25460 (130305) | 130305..130409 | - | 105 | Protein_160 | AAA family ATPase | - |
| M5R25_RS25465 (130411) | 130411..130734 | - | 324 | Protein_161 | IS21 family transposase IS21 | Transposase |
Similar MGE(s)
Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE.
| Detail | Organism | MGE type | Related TA | Genome accession | Coordinates | Mash distance |
|---|