Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 108356..108881 Replicon plasmid pMB5646_1
Accession NZ_CP103658
Organism Escherichia coli strain 3150

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag M5R25_RS25350 Protein ID WP_001159868.1
Coordinates 108356..108661 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag M5R25_RS25355 Protein ID WP_000813634.1
Coordinates 108663..108881 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R25_RS25335 (104266) 104266..105432 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
M5R25_RS25340 (106020) 106020..106775 - 756 WP_024946706.1 replication initiation protein RepE -
M5R25_RS25345 (107549) 107549..108355 - 807 WP_000016982.1 site-specific integrase -
M5R25_RS25350 (108356) 108356..108661 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
M5R25_RS25355 (108663) 108663..108881 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
M5R25_RS25360 (109448) 109448..109960 + 513 WP_000151784.1 hypothetical protein -
M5R25_RS25365 (109994) 109994..111127 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
M5R25_RS25370 (111294) 111294..112067 - 774 WP_000905949.1 hypothetical protein -
M5R25_RS25375 (112080) 112080..112580 - 501 WP_000528931.1 HEPN family nuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catB3 / blaOXA-1 / aac(6')-Ib-cr / aadA5 / qacE / sul1 / mph(A) / erm(B) / tet(B) / sitABCD iucA / iucB / iucC / iucD / iutA 1..131479 131479
- flank IS/Tn sitABCD - 112669..117171 4502


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T256066 WP_001159868.1 NZ_CP103658:c108661-108356 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT256066 WP_000813634.1 NZ_CP103658:c108881-108663 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References