MGE detailed information
MGE type: Conjugative plasmid MGE length: 128309 bp
Accession: NZ_CP095087 Location: 1..128309 Organism: Escherichia coli isolate EC5654 Replicon: plasmid pEC5654
Accession: NZ_CP095087 Location: 1..128309 Organism: Escherichia coli isolate EC5654 Replicon: plasmid pEC5654
Gene structure
The 5 kb flanking regions of this MGE are displayed.
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MUK77_RS24920 (155) | 155..412 | + | 258 | WP_001620838.1 | replication regulatory protein RepA | - |
| MUK77_RS24925 (646) | 646..720 | + | 75 | WP_001367777.1 | RepA leader peptide Tap | - |
| MUK77_RS24930 (713) | 713..1570 | + | 858 | WP_021515403.1 | incFII family plasmid replication initiator RepA | - |
| MUK77_RS24935 (2483) | 2483..2620 | + | 138 | Protein_3 | XRE family transcriptional regulator | - |
| MUK77_RS24940 (2640) | 2640..2903 | + | 264 | WP_001089474.1 | hypothetical protein | - |
| MUK77_RS24945 (2893) | 2893..3192 | + | 300 | WP_118253467.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| MUK77_RS24950 (3417) | 3417..4064 | - | 648 | WP_021515401.1 | hypothetical protein | - |
| MUK77_RS24955 (4256) | 4256..4435 | + | 180 | Protein_7 | entry exclusion protein 1 | - |
| MUK77_RS24960 (4516) | 4516..4944 | + | 429 | WP_021515400.1 | Exc2 family lipoprotein | - |
| MUK77_RS24965 (5492) | 5492..5776 | + | 285 | WP_024187480.1 | ribbon-helix-helix domain-containing protein | Antitoxin Detail |
| MUK77_RS24970 (5776) | 5776..6051 | + | 276 | WP_021515398.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin Detail |
| MUK77_RS24975 (6317) | 6317..6811 | - | 495 | Protein_11 | IS91 family transposase ISSbo1 | Transposase |
| MUK77_RS24980 (7088) | 7088..7963 | + | 876 | WP_021515396.1 | ChaN family lipoprotein | - |
| MUK77_RS24985 (7971) | 7971..8747 | + | 777 | WP_021515395.1 | energy transducer TonB | - |
| MUK77_RS24990 (8916) | 8916..11177 | + | 2262 | WP_021515394.1 | TonB-dependent receptor | - |
| MUK77_RS24995 (11246) | 11246..12421 | + | 1176 | WP_001020414.1 | putative protein YncE | VF |
| MUK77_RS25000 (12884) | 12884..14035 | - | 1152 | Protein_16 | IS3 family transposase | - |
| MUK77_RS25005 (14164) | 14164..15179 | + | 1016 | Protein_17 | IS3 family transposase | - |
| MUK77_RS25010 (15515) | 15515..16246 | + | 732 | WP_226147431.1 | Virulence regulon transcriptional activator VirF | VF |
| MUK77_RS25015 (16804) | 16804..18192 | + | 1389 | WP_021515390.1 | group II intron reverse transcriptase/maturase | - |
| MUK77_RS25020 (18370) | 18370..18786 | - | 417 | WP_021515389.1 | SdiA-regulated domain-containing protein | - |
| MUK77_RS25025 (19223) | 19223..19708 | - | 486 | WP_021515387.1 | Dr family adhesin structural subunit | - |
| MUK77_RS25030 (19816) | 19816..20238 | - | 423 | WP_021515386.1 | Protein AfaD | VF |
| MUK77_RS25035 (20254) | 20254..22806 | - | 2553 | WP_021515385.1 | F1 capsule-anchoring protein | VF |
| MUK77_RS25040 (22833) | 22833..23585 | - | 753 | WP_021515384.1 | Chaperone protein caf1M | VF |
| MUK77_RS25045 (23962) | 23962..24243 | - | 282 | Protein_25 | IS3 family transposase IS2 | Transposase |
| MUK77_RS25050 (24447) | 24447..25595 | - | 1149 | Protein_26 | IS3 family transposase | - |
| MUK77_RS25055 (26137) | 26137..26439 | + | 303 | WP_021515382.1 | Major pilu subunit operon regulatory protein PapB | VF |
| MUK77_RS25060 (26581) | 26581..27765 | + | 1185 | WP_021515381.1 | TolC family protein | - |
| MUK77_RS25065 (27762) | 27762..29909 | + | 2148 | WP_021515380.1 | Toxin RTX-I translocation ATP-binding protein | VF |
| MUK77_RS25070 (29969) | 29969..31216 | + | 1248 | WP_021515379.1 | HlyD family type I secretion periplasmic adaptor subunit | - |
| MUK77_RS25075 (31491) | 31491..42266 | + | 10776 | WP_244739779.1 | hypothetical protein | - |
| MUK77_RS25080 (42325) | 42325..44193 | + | 1869 | WP_021515447.1 | Lipase | VF |
| MUK77_RS25085 (44776) | 44776..45036 | + | 261 | WP_231528284.1 | LuxR C-terminal-related transcriptional regulator | - |
| MUK77_RS25090 (45164) | 45164..45358 | + | 195 | Protein_34 | IS66 family transposase ISEc23 | Transposase |
| MUK77_RS25095 (45434) | 45434..45694 | + | 261 | Protein_35 | IS3 family transposase ISSfl10 | Transposase |
| MUK77_RS25100 (45704) | 45704..45802 | - | 99 | Protein_36 | IS3 family transposase ISSfl10 | Transposase |
| MUK77_RS25105 (45858) | 45858..46597 | + | 740 | Protein_37 | IS3 family transposase IS629 | Transposase |
| MUK77_RS25110 (47270) | 47270..48778 | + | 1509 | WP_032144821.1 | group II intron reverse transcriptase/maturase | - |
| MUK77_RS25115 (48862) | 48862..49341 | + | 480 | Protein_39 | IS3 family transposase IS629 | Transposase |
| MUK77_RS25120 (49490) | 49490..51460 | - | 1971 | WP_021532814.1 | Hemin receptor | VF |
| MUK77_RS25125 (52920) | 52920..53192 | + | 273 | WP_021515441.1 | Major pilu subunit operon regulatory protein PapB | VF |
| MUK77_RS25130 (53539) | 53539..53678 | + | 140 | Protein_42 | IS110 family transposase | - |
| MUK77_RS25135 (53676) | 53676..53807 | + | 132 | Protein_43 | IS3 family transposase | - |
| MUK77_RS25140 (54033) | 54033..55202 | - | 1170 | WP_021515440.1 | hypothetical protein | - |
| MUK77_RS25145 (55564) | 55564..55752 | + | 189 | WP_032144810.1 | hypothetical protein | - |
| MUK77_RS25150 (55873) | 55873..56613 | + | 741 | WP_021515439.1 | tyrosine-type recombinase/integrase | - |
| MUK77_RS25155 (56892) | 56892..57869 | - | 978 | WP_021515438.1 | RepB family plasmid replication initiator protein | - |
| MUK77_RS25160 (59044) | 59044..59685 | - | 642 | WP_021515437.1 | hypothetical protein | - |
| MUK77_RS25165 (62006) | 62006..62224 | + | 219 | WP_000813634.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin Detail |
| MUK77_RS25170 (62226) | 62226..62531 | + | 306 | WP_001159871.1 | type II toxin-antitoxin system toxin CcdB | Toxin Detail |
| MUK77_RS25175 (62532) | 62532..63341 | + | 810 | WP_021515436.1 | site-specific integrase | - |
| MUK77_RS25180 (63479) | 63479..63754 | - | 276 | WP_000239529.1 | hypothetical protein | - |
| MUK77_RS25185 (63748) | 63748..64392 | - | 645 | WP_000633911.1 | AAA family ATPase | - |
| MUK77_RS25190 (64621) | 64621..65592 | + | 972 | WP_021515435.1 | plasmid segregation protein ParM | - |
| MUK77_RS25195 (65597) | 65597..65989 | + | 393 | WP_000340835.1 | plasmid partitioning/stability family protein | - |
| MUK77_RS25200 (66024) | 66024..67264 | - | 1241 | Protein_56 | translesion error-prone DNA polymerase V subunit UmuC | - |
| MUK77_RS25205 (67264) | 67264..67701 | - | 438 | WP_016245169.1 | translesion error-prone DNA polymerase V autoproteolytic subunit | - |
| MUK77_RS25210 (67698) | 67698..67946 | - | 249 | WP_000619112.1 | DinI-like family protein | - |
| MUK77_RS25215 (68364) | 68364..69266 | + | 903 | WP_077769867.1 | DUF1281 domain-containing protein | - |
| MUK77_RS25220 (69263) | 69263..69574 | + | 312 | WP_032144809.1 | hypothetical protein | - |
| MUK77_RS25225 (69651) | 69651..70334 | + | 684 | WP_000085884.1 | DNA methylase | - |
| MUK77_RS25230 (70335) | 70335..70556 | + | 222 | WP_021532813.1 | hypothetical protein | - |
| MUK77_RS25235 (70486) | 70486..71004 | + | 519 | WP_226147437.1 | DUF1380 family protein | - |
| MUK77_RS25240 (71049) | 71049..71819 | + | 771 | WP_021532812.1 | hypothetical protein | - |
| MUK77_RS25245 (72235) | 72235..72660 | + | 426 | WP_001546490.1 | antirestriction protein | - |
| MUK77_RS25250 (72707) | 72707..73129 | + | 423 | WP_000271739.1 | DUF1380 family protein | - |
| MUK77_RS25255 (73126) | 73126..73317 | + | 192 | WP_001027516.1 | hypothetical protein | - |
| MUK77_RS25260 (74354) | 74354..74584 | + | 231 | WP_000218642.1 | hypothetical protein | - |
| MUK77_RS25265 (74636) | 74636..75997 | + | 1362 | WP_021515428.1 | DUF3560 domain-containing protein | - |
| MUK77_RS25270 (76044) | 76044..76607 | + | 564 | WP_021515427.1 | class I SAM-dependent methyltransferase | - |
| MUK77_RS25275 (76607) | 76607..76861 | + | 255 | WP_244739775.1 | hypothetical protein | - |
| MUK77_RS25280 (76759) | 76759..77031 | + | 273 | WP_072130061.1 | hypothetical protein | - |
| MUK77_RS25285 (77504) | 77504..78025 | + | 522 | WP_231530616.1 | single-stranded DNA-binding protein | - |
| MUK77_RS25290 (78081) | 78081..78314 | + | 234 | WP_000005971.1 | DUF905 domain-containing protein | - |
| MUK77_RS25295 (78375) | 78375..80324 | + | 1950 | WP_021532809.1 | ParB/RepB/Spo0J family partition protein | - |
| MUK77_RS25300 (80379) | 80379..80813 | + | 435 | WP_000845928.1 | conjugation system SOS inhibitor PsiB | - |
| MUK77_RS25305 (80810) | 80810..81529 | + | 720 | WP_021515423.1 | plasmid SOS inhibition protein A | - |
| MUK77_RS25310 (81526) | 81526..81840 | + | 315 | WP_001562885.1 | hypothetical protein | - |
| - (81797) | 81797..81982 | + | 186 | NuclAT_0 | - | Antitoxin Detail |
| - (81797) | 81797..81982 | + | 186 | NuclAT_0 | - | Antitoxin Detail |
| - (81797) | 81797..81982 | + | 186 | NuclAT_0 | - | Antitoxin Detail |
| - (81797) | 81797..81982 | + | 186 | NuclAT_0 | - | Antitoxin Detail |
| MUK77_RS25315 (81990) | 81990..82142 | + | 153 | Protein_79 | DUF5431 family protein | - |
| MUK77_RS25320 (82087) | 82087..82209 | + | 123 | WP_223200913.1 | Hok/Gef family protein | Toxin Detail |
| MUK77_RS25325 (82510) | 82510..82759 | - | 250 | Protein_81 | hypothetical protein | - |
| MUK77_RS25330 (83055) | 83055..83378 | + | 324 | WP_000533253.1 | hypothetical protein | - |
| MUK77_RS25335 (83437) | 83437..83679 | + | 243 | WP_000540588.1 | hypothetical protein | - |
| MUK77_RS25340 (83945) | 83945..84211 | + | 267 | WP_071828223.1 | hypothetical protein | - |
| MUK77_RS25345 (84682) | 84682..84978 | + | 297 | WP_001272251.1 | hypothetical protein | - |
| MUK77_RS25350 (85088) | 85088..85909 | + | 822 | WP_231530617.1 | DUF932 domain-containing protein | - |
| MUK77_RS25355 (86206) | 86206..86808 | - | 603 | WP_077761845.1 | hypothetical protein | VF |
| MUK77_RS25360 (87139) | 87139..87522 | + | 384 | WP_001151562.1 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| MUK77_RS25365 (87716) | 87716..88402 | + | 687 | WP_021515421.1 | PAS domain-containing protein | - |
| MUK77_RS25370 (88496) | 88496..88723 | + | 228 | WP_001254388.1 | conjugal transfer relaxosome protein TraY | - |
| MUK77_RS25375 (88757) | 88757..89119 | + | 363 | WP_001098993.1 | type IV conjugative transfer system pilin TraA | - |
| MUK77_RS25380 (89134) | 89134..89445 | + | 312 | WP_000012100.1 | type IV conjugative transfer system protein TraL | - |
| MUK77_RS25385 (89467) | 89467..90033 | + | 567 | WP_000399805.1 | type IV conjugative transfer system protein TraE | - |
| MUK77_RS25390 (90020) | 90020..90748 | + | 729 | WP_001230787.1 | type-F conjugative transfer system secretin TraK | - |
| MUK77_RS25395 (90748) | 90748..92199 | + | 1452 | WP_021515420.1 | F-type conjugal transfer pilus assembly protein TraB | - |
| MUK77_RS25400 (92189) | 92189..92755 | + | 567 | WP_021515419.1 | conjugal transfer pilus-stabilizing protein TraP | - |
| MUK77_RS25405 (92742) | 92742..93065 | + | 324 | WP_021515418.1 | conjugal transfer protein TrbD | - |
| MUK77_RS25410 (93058) | 93058..93567 | + | 510 | WP_001526036.1 | hypothetical protein | - |
| MUK77_RS25415 (93560) | 93560..94072 | + | 513 | WP_001526035.1 | type IV conjugative transfer system lipoprotein TraV | - |
| MUK77_RS25420 (94207) | 94207..94428 | + | 222 | WP_001278978.1 | conjugal transfer protein TraR | - |
| MUK77_RS25425 (94421) | 94421..94894 | + | 474 | WP_021515417.1 | hypothetical protein | - |
| MUK77_RS25430 (94972) | 94972..95190 | + | 219 | WP_001377841.1 | hypothetical protein | - |
| MUK77_RS25435 (95218) | 95218..95565 | + | 348 | WP_021515416.1 | hypothetical protein | - |
| MUK77_RS25440 (95691) | 95691..98321 | + | 2631 | WP_012917677.1 | type IV secretion system protein TraC | - |
| MUK77_RS25445 (98318) | 98318..98704 | + | 387 | WP_000214084.1 | type-F conjugative transfer system protein TrbI | - |
| MUK77_RS25450 (98701) | 98701..99333 | + | 633 | WP_001203728.1 | type-F conjugative transfer system protein TraW | - |
| MUK77_RS25455 (99330) | 99330..100322 | + | 993 | WP_000830838.1 | conjugal transfer pilus assembly protein TraU | - |
| MUK77_RS25460 (100352) | 100352..100657 | + | 306 | WP_000224416.1 | hypothetical protein | - |
| MUK77_RS25465 (100666) | 100666..101304 | + | 639 | WP_001080256.1 | type-F conjugative transfer system pilin assembly protein TrbC | - |
| MUK77_RS25470 (101301) | 101301..103151 | + | 1851 | WP_000821856.1 | type-F conjugative transfer system mating-pair stabilization protein TraN | - |
| MUK77_RS25475 (103178) | 103178..103435 | + | 258 | WP_000864353.1 | conjugal transfer protein TrbE | - |
| MUK77_RS25480 (103428) | 103428..104171 | + | 744 | WP_001030371.1 | type-F conjugative transfer system pilin assembly protein TraF | - |
| MUK77_RS25485 (104185) | 104185..104529 | + | 345 | WP_000556796.1 | conjugal transfer protein TrbA | - |
| MUK77_RS25490 (104648) | 104648..104932 | + | 285 | WP_000624194.1 | type-F conjugative transfer system pilin chaperone TraQ | - |
| MUK77_RS25495 (104919) | 104919..105464 | + | 546 | WP_000059831.1 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | - |
| MUK77_RS25500 (105394) | 105394..105741 | + | 348 | WP_001309242.1 | P-type conjugative transfer protein TrbJ | - |
| MUK77_RS25505 (105722) | 105722..106114 | + | 393 | WP_000660699.1 | F-type conjugal transfer protein TrbF | - |
| MUK77_RS25510 (106101) | 106101..107474 | + | 1374 | WP_000944331.1 | conjugal transfer pilus assembly protein TraH | - |
| MUK77_RS25515 (107471) | 107471..110293 | + | 2823 | WP_244739777.1 | conjugal transfer mating-pair stabilization protein TraG | - |
| MUK77_RS25520 (110308) | 110308..110811 | + | 504 | WP_231530619.1 | surface exclusion protein | - |
| MUK77_RS25525 (110843) | 110843..111574 | + | 732 | WP_024187483.1 | conjugal transfer complement resistance protein TraT | - |
| MUK77_RS25530 (111777) | 111777..112064 | + | 288 | Protein_122 | hypothetical protein | - |
| MUK77_RS25535 (112154) | 112154..112900 | - | 747 | WP_001016257.1 | IS21 family transposase ISEc12 | Transposase |
| MUK77_RS25540 (112915) | 112915..114456 | - | 1542 | WP_001620815.1 | IS21 family transposase ISEc12 | Transposase |
| MUK77_RS25545 (114649) | 114649..115101 | + | 453 | Protein_125 | hypothetical protein | - |
| MUK77_RS25550 (115152) | 115152..117413 | + | 2262 | WP_242789071.1 | type IV conjugative transfer system coupling protein TraD | - |
| MUK77_RS25555 (117413) | 117413..122683 | + | 5271 | WP_021515408.1 | Multifunctional conjugation protein TraI | Relaxase |
| MUK77_RS25560 (122703) | 122703..123449 | + | 747 | WP_021515407.1 | conjugal transfer pilus acetylase TraX | - |
| MUK77_RS25565 (123504) | 123504..124064 | + | 561 | WP_001620825.1 | fertility inhibition protein FinO | - |
| MUK77_RS25570 (124199) | 124199..124411 | + | 213 | WP_032144806.1 | hypothetical protein | - |
| MUK77_RS25575 (124656) | 124656..125117 | + | 462 | WP_021515406.1 | thermonuclease family protein | - |
| MUK77_RS25580 (125163) | 125163..125372 | + | 210 | WP_001333231.1 | hemolysin expression modulator Hha | - |
| MUK77_RS25585 (125410) | 125410..126096 | + | 687 | WP_001620830.1 | DUF2726 domain-containing protein | - |
| MUK77_RS25590 (126093) | 126093..126401 | + | 309 | WP_001620832.1 | hypothetical protein | - |
| - (126576) | 126576..126635 | - | 60 | NuclAT_1 | - | - |
| - (126576) | 126576..126635 | - | 60 | NuclAT_1 | - | - |
| - (126576) | 126576..126635 | - | 60 | NuclAT_1 | - | - |
| - (126576) | 126576..126635 | - | 60 | NuclAT_1 | - | - |
| MUK77_RS25595 (126680) | 126680..126829 | + | 150 | WP_021515405.1 | Hok/Gef family protein | - |
| MUK77_RS25600 (127464) | 127464..128162 | + | 699 | WP_021515404.1 | acid phosphatase AphA | - |
Similar MGE(s)
Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE.
| Detail | Organism | MGE type | Related TA | Genome accession | Coordinates | Mash distance |
|---|