Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 62006..62531 Replicon plasmid pEC5654
Accession NZ_CP095087
Organism Escherichia coli isolate EC5654

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag MUK77_RS25170 Protein ID WP_001159871.1
Coordinates 62226..62531 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag MUK77_RS25165 Protein ID WP_000813634.1
Coordinates 62006..62224 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUK77_RS25160 (59044) 59044..59685 - 642 WP_021515437.1 hypothetical protein -
MUK77_RS25165 (62006) 62006..62224 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
MUK77_RS25170 (62226) 62226..62531 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
MUK77_RS25175 (62532) 62532..63341 + 810 WP_021515436.1 site-specific integrase -
MUK77_RS25180 (63479) 63479..63754 - 276 WP_000239529.1 hypothetical protein -
MUK77_RS25185 (63748) 63748..64392 - 645 WP_000633911.1 AAA family ATPase -
MUK77_RS25190 (64621) 64621..65592 + 972 WP_021515435.1 plasmid segregation protein ParM -
MUK77_RS25195 (65597) 65597..65989 + 393 WP_000340835.1 plasmid partitioning/stability family protein -
MUK77_RS25200 (66024) 66024..67264 - 1241 Protein_56 translesion error-prone DNA polymerase V subunit UmuC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..128309 128309


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T241265 WP_001159871.1 NZ_CP095087:62226-62531 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T241265 NZ_LT963412:14456-14833 [Pseudomonas syringae]
GTGAAAGGCGTCCTGGTAGACACCTCCGTTTGGGTTGAGCACTTTCGCAACAACAGTCCAGAACTGGTGAACTTGCTCAG
CCAGGACCGGGTACTCATACACCCCATGGTGATCGGAGAACTGGCGTGTGGTACGCCACCTGATCGTTCGAACACCTTGA
CCGACCTTGGCGACCTACGAGGTGCACAACAACCCACGGTGAGTGAAGTCATCGCCTTTCTGAACACCCACAAGCTCTAT
GGCCTGGGCTGTGGGCTGGTGGATATGACGCTCCTGGCCTCCGCGTTATTGAGCGGCACAGCGCTTTGGACGTTAGACAA
GCGCCTGGAGCGTTTGGCTAGTCGCATGGCCGTGTCTTACCAGCCTCCTACTCACTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT241265 WP_000813634.1 NZ_CP095087:62006-62224 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT241265 NZ_LT963412:14259-14459 [Pseudomonas syringae]
ATGAGAACGACCGTCACCATCGACGATGCGTTGTACCAACGTGCTCTGGAAGTCGCCGATCCGGCGATGGATAAGGCCGA
CCTGTTCCGTGAAGCCGTTCAGACGTTTGTGCGCATTCAAGCAGCTAAACGATTGATGGCCTTAGGGGCCACACTGCCAA
CGATGGAAGACATCGCCCGCCGACACGAGAAGGCACTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References