Detailed information of TA system TA241264

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 5492..6051
• Replicon: plasmid pEC5654
• Accession: NZ_CP095087
• Organism: Escherichia coli isolate EC5654

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A376ZSB3
• Locus tag: MUK77_RS24970
• Protein ID: WP_021515398.1
• Coordinates: 5776..6051 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MUK77_RS24965
• Protein ID: WP_024187480.1
• Coordinates: 5492..5776 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUK77_RS24925 (646)	646..720	+	75	WP_001367777.1	RepA leader peptide Tap	-
MUK77_RS24930 (713)	713..1570	+	858	WP_021515403.1	incFII family plasmid replication initiator RepA	-
MUK77_RS24935 (2483)	2483..2620	+	138	Protein_3	XRE family transcriptional regulator	-
MUK77_RS24940 (2640)	2640..2903	+	264	WP_001089474.1	hypothetical protein	-
MUK77_RS24945 (2893)	2893..3192	+	300	WP_118253467.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MUK77_RS24950 (3417)	3417..4064	-	648	WP_021515401.1	hypothetical protein	-
MUK77_RS24955 (4256)	4256..4435	+	180	Protein_7	entry exclusion protein 1	-
MUK77_RS24960 (4516)	4516..4944	+	429	WP_021515400.1	Exc2 family lipoprotein	-
MUK77_RS24965 (5492)	5492..5776	+	285	WP_024187480.1	ribbon-helix-helix domain-containing protein	Antitoxin
MUK77_RS24970 (5776)	5776..6051	+	276	WP_021515398.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MUK77_RS24975 (6317)	6317..6811	-	495	Protein_11	IS91 family transposase	-
MUK77_RS24980 (7088)	7088..7963	+	876	WP_021515396.1	ChaN family lipoprotein	-
MUK77_RS24985 (7971)	7971..8747	+	777	WP_021515395.1	energy transducer TonB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	senB	1..128309	128309
	-	flank	IS/Tn	-	-	6317..6583	266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10778.55 Da
• Isoelectric Point: 10.2344

>T241264 WP_021515398.1 NZ_CP095087:5776-6051 [Escherichia coli]
MELKWTSKALSNLARLYDFLVLASKPAAARMVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T241264 NZ_LT963411:c11799-11473 [Pseudomonas syringae]
ATGATGCGAGGTGACCTAGTCACCGTCGCCATGCAAGGTGACTTTGGTAAACCAAGACCCGCCTTGGTAATTCAGGCAAA
CCAATTCAGCGAACACACCAGCGTTACCGTGCTGCCAGTGACCAGCACCCTAGTCGCAGCACCGCTGCTGCGCATCACCG
TTCAGCCGACCGCAGAAAACGGCTTGCAGAAGCCTTCTCAGGTCATGCTAGACAAGACCATGACCCTCAAGCGTGAAAAA
ATAGGCCCGACTTTTGGGCATATCGGAGTGGATTCAATGGTGGAGGTAGAGCGCTGTTTAGCCGTGTTCTTGGGTATCGC
CAATTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10470.89 Da
• Isoelectric Point: 5.6558

>AT241264 WP_024187480.1 NZ_CP095087:5492-5776 [Escherichia coli]
IQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQIIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT241264 NZ_LT963411:c12020-11796 [Pseudomonas syringae]
ATGCCTACATCCCATGTAAATGCCCGCGTTCAACGCCATCGCGACGCGCTGCGTGCGGCTGGCCTACGGCCCATTCAGAT
TTGGGTTCCCGATACCCGGCGGCCAGACTTTGCACAGGAGTGCCATCGACAGTGCCTGTTGGTTGCTCAGGCAGACATGG
CCAATACCTATATGCAGGGGTTGATGGACGAAGCCTTAGACGATGTTGAGGGTTGGACGCCATGA

Structures

Toxin

• AlphaFold DB: A0A376ZSB3