Detailed information of TA system TA241266

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 81797..82209
• Replicon: plasmid pEC5654
• Accession: NZ_CP095087
• Organism: Escherichia coli isolate EC5654

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: MUK77_RS25320
• Protein ID: WP_223200913.1
• Coordinates: 82087..82209 (+)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	81797..81982 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUK77_RS25285 (77504)	77504..78025	+	522	WP_231530616.1	single-stranded DNA-binding protein	-
MUK77_RS25290 (78081)	78081..78314	+	234	WP_000005971.1	DUF905 domain-containing protein	-
MUK77_RS25295 (78375)	78375..80324	+	1950	WP_021532809.1	ParB/RepB/Spo0J family partition protein	-
MUK77_RS25300 (80379)	80379..80813	+	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
MUK77_RS25305 (80810)	80810..81529	+	720	WP_021515423.1	plasmid SOS inhibition protein A	-
MUK77_RS25310 (81526)	81526..81840	+	315	WP_001562885.1	hypothetical protein	-
- (81797)	81797..81982	+	186	NuclAT_0	-	Antitoxin
- (81797)	81797..81982	+	186	NuclAT_0	-	Antitoxin
- (81797)	81797..81982	+	186	NuclAT_0	-	Antitoxin
- (81797)	81797..81982	+	186	NuclAT_0	-	Antitoxin
MUK77_RS25315 (81990)	81990..82142	+	153	Protein_79	DUF5431 family protein	-
MUK77_RS25320 (82087)	82087..82209	+	123	WP_223200913.1	Hok/Gef family protein	Toxin
MUK77_RS25325 (82510)	82510..82759	-	250	Protein_81	hypothetical protein	-
MUK77_RS25330 (83055)	83055..83378	+	324	WP_000533253.1	hypothetical protein	-
MUK77_RS25335 (83437)	83437..83679	+	243	WP_000540588.1	hypothetical protein	-
MUK77_RS25340 (83945)	83945..84211	+	267	WP_071828223.1	hypothetical protein	-
MUK77_RS25345 (84682)	84682..84978	+	297	WP_001272251.1	hypothetical protein	-
MUK77_RS25350 (85088)	85088..85909	+	822	WP_231530617.1	DUF932 domain-containing protein	-
MUK77_RS25355 (86206)	86206..86808	-	603	WP_077761845.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	senB	1..128309	128309

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4538.38 Da
• Isoelectric Point: 8.2691

>T241266 WP_223200913.1 NZ_CP095087:82087-82209 [Escherichia coli]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 123 bp

>T241266 NZ_LT963412:c37614-37342 [Pseudomonas syringae]
GTGCTAGTTGAGTGGCTTCCACAAGCTTTGGAGGCACTGGAGGAAATCGCCGGCTACCTAGCCGAGCGCAACCCTTATGC
TGCCGAGCAGATGCAGGCCACCCTTGAGGCCACGGTCGAGGCTCTCCCCCTGCACCCTTACATCCACCGTCCCGGCCGTG
TTCCAGGAACGCGCGAAGCGGTTGTGCATCCCAACTACCTGGTTGTCTACCGCGTCGGTACAGAGCGCATCAGCATCTTG
GACGTCCTGCACGCCCGCCAAGAGTACCCCTAG

Antitoxin

Nucleotide

• Length: 186 bp

>AT241266 NZ_CP095087:81797-81982 [Escherichia coli]
CCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGGATATGAGCTATGATGTG
CCCGGCGCTTGAGGCTTTTTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGCAGAAAGCCCCGT
AGTTAATTTTCATTAACCCACGAGGC

Structures

Antitoxin