MGE detailed information
MGE type: Non-Mobilizable plasmid MGE length: 104844 bp
Accession: NZ_CP062161 Location: 1..104844 Organism: Escherichia coli strain 20R2R Replicon: plasmid punnamed1
Accession: NZ_CP062161 Location: 1..104844 Organism: Escherichia coli strain 20R2R Replicon: plasmid punnamed1
Gene structure
The 5 kb flanking regions of this MGE are displayed.
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IEM32_RS27175 () | 1..587 | - | 587 | Protein_0 | ParB/RepB/Spo0J family plasmid partition protein | - |
| IEM32_RS27180 () | 587..1768 | - | 1182 | Protein_1 | plasmid-partitioning protein SopA | - |
| IEM32_RS27185 () | 2356..2694 | - | 339 | WP_071525396.1 | RepB family plasmid replication initiator protein | - |
| IEM32_RS27190 () | 2656..3869 | + | 1214 | WP_162829202.1 | IS3 family transposase IS1203 | Transposase |
| IEM32_RS27195 () | 4592..5398 | - | 807 | WP_000016989.1 | site-specific integrase | - |
| IEM32_RS27200 () | 5399..5704 | - | 306 | WP_001159868.1 | type II toxin-antitoxin system toxin CcdB | Toxin Detail |
| IEM32_RS27205 () | 5706..5924 | - | 219 | WP_000813634.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin Detail |
| IEM32_RS27210 () | 6383..7066 | + | 684 | WP_010891291.1 | DNA-binding protein | - |
| IEM32_RS27215 () | 7063..7683 | + | 621 | WP_001248529.1 | hypothetical protein | - |
| IEM32_RS27220 () | 7680..7880 | + | 201 | WP_000708307.1 | hypothetical protein | - |
| IEM32_RS27225 () | 8288..9265 | + | 978 | WP_000361615.1 | RepB family plasmid replication initiator protein | - |
| IEM32_RS27230 () | 9550..10290 | - | 741 | WP_001066920.1 | IS91 family transposase ISMno23 | Transposase |
| IEM32_RS27235 () | 10411..10641 | - | 231 | WP_010891288.1 | hypothetical protein | - |
| IEM32_RS27240 () | 10772..11264 | - | 493 | Protein_13 | MarR family transcriptional regulator | - |
| IEM32_RS27245 () | 11385..12824 | - | 1440 | WP_001213545.1 | Hemolysin secretion protein D, chromosomal | VF |
| IEM32_RS27250 () | 12828..14948 | - | 2121 | WP_000987091.1 | Alpha-hemolysin translocation ATP-binding protein HlyB | VF |
| IEM32_RS27255 () | 14998..17994 | - | 2997 | WP_000217739.1 | Hemolysin, chromosomal | VF |
| IEM32_RS27260 () | 17996..18511 | - | 516 | WP_000839950.1 | Hemolysin-activating lysine-acyltransferase HlyC | VF |
| IEM32_RS27265 () | 19052..20223 | - | 1172 | Protein_18 | IS3 family transposase ISLad1 | Transposase |
| IEM32_RS27270 () | 20402..20803 | - | 402 | WP_000971918.1 | GspS family T2SS pilot lipoprotein variant EptO | - |
| IEM32_RS27275 () | 20895..21728 | - | 834 | WP_000096786.1 | Type 4 prepilin-like proteins leader peptide-processing enzyme | VF |
| IEM32_RS27280 () | 21786..22298 | - | 513 | WP_001004187.1 | Type II secretion system protein M | VF |
| IEM32_RS27285 () | 22285..23457 | - | 1173 | WP_071525076.1 | Type II secretion system protein L | VF |
| IEM32_RS27290 () | 23496..24473 | - | 978 | WP_000776550.1 | Putative type II secretion system protein K | VF |
| IEM32_RS27295 () | 24470..25069 | - | 600 | WP_000082782.1 | Type II secretion system protein J | VF |
| IEM32_RS27300 () | 25066..25413 | - | 348 | WP_000173396.1 | Type II secretion system protein I | VF |
| IEM32_RS27305 () | 25428..25982 | - | 555 | WP_000082929.1 | Putative type II secretion system protein H | VF |
| IEM32_RS27310 () | 25979..26413 | - | 435 | WP_001231211.1 | Type II secretion system protein G | VF |
| IEM32_RS27315 () | 26444..27667 | - | 1224 | WP_001173152.1 | Type II secretion system protein F | VF |
| IEM32_RS27320 () | 27669..29174 | - | 1506 | WP_000092338.1 | Type II secretion system protein E | VF |
| IEM32_RS27325 () | 29174..31141 | - | 1968 | WP_001302177.1 | Type II secretion system protein D | VF |
| IEM32_RS27330 () | 31142..32017 | - | 876 | WP_001302175.1 | Type II secretion system protein C | VF |
| IEM32_RS27335 () | 32104..34800 | - | 2697 | WP_001358886.1 | Metalloprotease StcE | VF |
| IEM32_RS27340 () | 35291..35377 | + | 87 | Protein_33 | IS3 family transposase ISEhe3 | Transposase |
| IEM32_RS27345 () | 35481..35669 | - | 189 | Protein_34 | hypothetical protein | - |
| IEM32_RS27350 () | 35722..36720 | - | 999 | WP_001302181.1 | Lipid A biosynthesis myristoyltransferase | VF |
| IEM32_RS27355 () | 36794..38515 | - | 1722 | WP_000550559.1 | phosphoethanolamine transferase CptA | - |
| IEM32_RS27360 () | 38605..39711 | - | 1107 | WP_000975743.1 | glycosyltransferase family 4 protein | - |
| IEM32_RS27365 () | 39711..40532 | - | 822 | WP_001302199.1 | polysaccharide deacetylase family protein | - |
| IEM32_RS27370 () | 41974..42455 | + | 482 | Protein_39 | IS1 family transposase IS1N | Transposase |
| IEM32_RS27375 () | 42711..46613 | - | 3903 | WP_001034100.1 | Serine protease EspP | VF |
| IEM32_RS27380 () | 46794..47782 | - | 989 | Protein_41 | IS3 family transposase IS629 | Transposase |
| IEM32_RS27385 () | 47839..48228 | - | 390 | WP_001172748.1 | cytochrome b562 family protein | - |
| IEM32_RS27390 () | 48272..50482 | - | 2211 | WP_000592771.1 | Catalase-peroxidase | VF |
| IEM32_RS27395 () | 50659..50844 | + | 186 | WP_001302179.1 | hypothetical protein | - |
| IEM32_RS27400 () | 50844..51260 | + | 417 | Protein_45 | IS91 family transposase | - |
| IEM32_RS27405 () | 51260..51826 | + | 567 | Protein_46 | IS3 family transposase ISSd1 | Transposase |
| IEM32_RS27410 () | 51870..52085 | + | 216 | Protein_47 | IS91 family transposase ISEc37 | Transposase |
| IEM32_RS27415 () | 52185..52391 | - | 207 | WP_001105064.1 | hypothetical protein | - |
| IEM32_RS27420 () | 52486..52761 | - | 276 | WP_000421248.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin Detail |
| IEM32_RS27425 () | 52761..53045 | - | 285 | WP_001178089.1 | ribbon-helix-helix domain-containing protein | Antitoxin Detail |
| IEM32_RS27430 () | 53957..54814 | - | 858 | WP_000130945.1 | incFII family plasmid replication initiator RepA | - |
| IEM32_RS27435 () | 54807..54881 | - | 75 | WP_001370046.1 | RepA leader peptide Tap | - |
| IEM32_RS27440 () | 55117..55371 | - | 255 | WP_000083831.1 | replication regulatory protein RepA | - |
| IEM32_RS27445 () | 55611..55949 | - | 339 | WP_000766796.1 | hypothetical protein | - |
| IEM32_RS27450 () | 55987..56196 | - | 210 | WP_001302200.1 | hemolysin expression modulator Hha | - |
| IEM32_RS27455 () | 56242..56703 | - | 462 | WP_001233853.1 | thermonuclease family protein | - |
| IEM32_RS27460 () | 56948..57160 | - | 213 | WP_001302189.1 | ANR family transcriptional regulator | - |
| IEM32_RS27465 () | 57293..57853 | - | 561 | WP_000840472.1 | fertility inhibition protein FinO | - |
| IEM32_RS27470 () | 57956..58816 | - | 861 | WP_000704522.1 | putative protein YcjY | VF |
| IEM32_RS27475 () | 58875..59621 | - | 747 | WP_000205762.1 | conjugal transfer pilus acetylase TraX | - |
| IEM32_RS27480 () | 59641..61029 | - | 1389 | Protein_61 | Multifunctional conjugation protein TraI | Relaxase |
| IEM32_RS27485 () | 61086..61337 | + | 252 | Protein_62 | IS3 family transposase IS1203 | Transposase |
| IEM32_RS27490 () | 61344..61514 | + | 171 | Protein_63 | IS3 family transposase IS1203 | Transposase |
| IEM32_RS27495 () | 61582..61806 | - | 225 | Protein_64 | IS3 family transposase IS3H | Transposase |
| IEM32_RS27500 () | 61880..71389 | - | 9510 | WP_000581721.1 | Toxin B | VF |
| IEM32_RS27505 () | 71496..71648 | - | 153 | Protein_66 | IS1 family transposase | - |
| IEM32_RS27510 () | 71645..71765 | - | 121 | Protein_67 | IS21 family transposase ISEc62 | Transposase |
| IEM32_RS27515 () | 71805..72068 | - | 264 | Protein_68 | IS21 family transposase ISEc62 | Transposase |
| IEM32_RS27520 () | 72162..72511 | + | 350 | Protein_69 | IS3 family transposase IS3H | Transposase |
| IEM32_RS27525 () | 73176..73607 | - | 432 | WP_001453090.1 | hypothetical protein | - |
| IEM32_RS27530 () | 73675..75696 | - | 2022 | Protein_71 | hypothetical protein | Relaxase |
| IEM32_RS27535 () | 75830..75988 | - | 159 | WP_001302184.1 | type I toxin-antitoxin system Hok family toxin | - |
| - () | 76053..76251 | - | 199 | NuclAT_0 | - | - |
| - () | 76053..76251 | - | 199 | NuclAT_0 | - | - |
| - () | 76053..76251 | - | 199 | NuclAT_0 | - | - |
| - () | 76053..76251 | - | 199 | NuclAT_0 | - | - |
| IEM32_RS27540 () | 76220..76981 | - | 762 | WP_001276250.1 | plasmid SOS inhibition protein A | - |
| IEM32_RS27545 () | 76978..77412 | - | 435 | WP_000845908.1 | conjugation system SOS inhibitor PsiB | - |
| IEM32_RS27550 () | 77467..79425 | - | 1959 | WP_000117168.1 | ParB/RepB/Spo0J family partition protein | - |
| IEM32_RS27555 () | 79491..79724 | - | 234 | WP_000005995.1 | DUF905 family protein | - |
| IEM32_RS27560 () | 79781..80233 | - | 453 | WP_000290823.1 | single-stranded DNA-binding protein | - |
| IEM32_RS27565 () | 80259..80465 | - | 207 | WP_000547965.1 | hypothetical protein | - |
| IEM32_RS27570 () | 80535..80990 | + | 456 | WP_001358896.1 | hypothetical protein | - |
| IEM32_RS27575 () | 81076..81639 | - | 564 | WP_001302171.1 | class I SAM-dependent methyltransferase | - |
| IEM32_RS27580 () | 81686..83047 | - | 1362 | WP_000199442.1 | DUF3560 domain-containing protein | - |
| IEM32_RS27585 () | 83099..83329 | - | 231 | WP_000218642.1 | hypothetical protein | - |
| IEM32_RS27590 () | 83846..84355 | + | 510 | WP_032245379.1 | hypothetical protein | - |
| IEM32_RS27595 () | 84325..84516 | - | 192 | WP_001027495.1 | hypothetical protein | - |
| IEM32_RS27600 () | 84513..84935 | - | 423 | WP_000271685.1 | DUF1380 family protein | - |
| IEM32_RS27605 () | 84982..85284 | - | 303 | WP_010891293.1 | antirestriction protein | - |
| IEM32_RS27610 () | 85825..86601 | - | 777 | WP_001005037.1 | hypothetical protein | - |
| IEM32_RS27615 () | 86647..87201 | - | 555 | WP_001358893.1 | DUF1380 family protein | - |
| IEM32_RS27620 () | 87095..87316 | - | 222 | WP_001104869.1 | hypothetical protein | - |
| IEM32_RS27625 () | 87317..88000 | - | 684 | WP_000085945.1 | DNA methylase | - |
| IEM32_RS27630 () | 88077..88382 | - | 306 | WP_010891292.1 | hypothetical protein | - |
| IEM32_RS27635 () | 88386..89288 | - | 903 | WP_000273919.1 | DUF1281 domain-containing protein | - |
| IEM32_RS27640 () | 89365..91089 | - | 1725 | WP_000138832.1 | group II intron reverse transcriptase/maturase | - |
| IEM32_RS27645 () | 92389..93360 | - | 972 | WP_000817031.1 | ParB/RepB/Spo0J family plasmid partition protein | - |
| IEM32_RS27650 () | 93360..94526 | - | 1167 | WP_000772446.1 | Sporulation initiation inhibitor protein Soj | VF |
| IEM32_RS27655 () | 95114..95452 | - | 339 | WP_071525396.1 | RepB family plasmid replication initiator protein | - |
| IEM32_RS27660 () | 95414..96627 | + | 1214 | WP_162829202.1 | IS3 family transposase IS1203 | Transposase |
| IEM32_RS27665 () | 97350..98156 | - | 807 | WP_000016989.1 | site-specific integrase | - |
| IEM32_RS27670 () | 98157..98462 | - | 306 | WP_001159868.1 | type II toxin-antitoxin system toxin CcdB | Toxin Detail |
| IEM32_RS27675 () | 98464..98682 | - | 219 | WP_000813634.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin Detail |
| IEM32_RS27680 () | 99141..99824 | + | 684 | WP_010891291.1 | DNA-binding protein | - |
| IEM32_RS27685 () | 99821..100441 | + | 621 | WP_001248529.1 | hypothetical protein | - |
| IEM32_RS27690 () | 100438..100638 | + | 201 | WP_000708307.1 | hypothetical protein | - |
| IEM32_RS27695 () | 101046..102023 | + | 978 | WP_000361615.1 | RepB family plasmid replication initiator protein | - |
| IEM32_RS27700 () | 102308..103053 | - | 746 | Protein_105 | site-specific integrase | - |
| IEM32_RS27705 () | 103174..103404 | - | 231 | WP_191667005.1 | serine/threonine protein kinase | - |
| IEM32_RS27710 () | 103538..104038 | - | 501 | Protein_107 | MarR family transcriptional regulator | - |
| IEM32_RS27715 () | 104160..104843 | - | 684 | Protein_108 | Leukotoxin export protein LtxD | VF |
Similar MGE(s)
Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE.
| Detail | Organism | MGE type | Related TA | Genome accession | Coordinates | Mash distance |
|---|