Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 98157..98682 Replicon plasmid punnamed1
Accession NZ_CP062161
Organism Escherichia coli strain 20R2R

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag IEM32_RS27670 Protein ID WP_001159868.1
Coordinates 98157..98462 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag IEM32_RS27675 Protein ID WP_000813634.1
Coordinates 98464..98682 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEM32_RS27650 93360..94526 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
IEM32_RS27655 95114..95452 - 339 WP_071525396.1 RepB family plasmid replication initiator protein -
IEM32_RS27660 95414..96627 + 1214 WP_162829202.1 IS3 family transposase -
IEM32_RS27665 97350..98156 - 807 WP_000016989.1 site-specific integrase -
IEM32_RS27670 98157..98462 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
IEM32_RS27675 98464..98682 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
IEM32_RS27680 99141..99824 + 684 WP_010891291.1 DNA-binding protein -
IEM32_RS27685 99821..100441 + 621 WP_001248529.1 hypothetical protein -
IEM32_RS27690 100438..100638 + 201 WP_000708307.1 hypothetical protein -
IEM32_RS27695 101046..102023 + 978 WP_000361615.1 RepB family plasmid replication initiator protein -
IEM32_RS27700 102308..103053 - 746 Protein_105 site-specific integrase -
IEM32_RS27705 103174..103404 - 231 WP_191667005.1 serine/threonine protein kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hlyD / hlyB / hlyA / hlyC / exeG / exeE / stcE / espP / toxB / hlyD / hlyD / hlyD 1..104844 104844


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T173465 WP_001159868.1 NZ_CP062161:c98462-98157 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T173465 NZ_CP080223:979430-979765 [Escherichia coli O141:H4]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT173465 WP_000813634.1 NZ_CP062161:c98682-98464 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT173465 NZ_CP080223:979182-979430 [Escherichia coli O141:H4]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References