Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 52486..53045 Replicon plasmid punnamed1
Accession NZ_CP062161
Organism Escherichia coli strain 20R2R

Toxin (Protein)


Gene name relE Uniprot ID O82928
Locus tag IEM32_RS27420 Protein ID WP_000421248.1
Coordinates 52486..52761 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag IEM32_RS27425 Protein ID WP_001178089.1
Coordinates 52761..53045 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEM32_RS27385 47839..48228 - 390 WP_001172748.1 cytochrome b562 family protein -
IEM32_RS27390 48272..50482 - 2211 WP_000592771.1 catalase/peroxidase KatP -
IEM32_RS27395 50659..50844 + 186 WP_001302179.1 hypothetical protein -
IEM32_RS27400 50844..51260 + 417 Protein_45 IS91 family transposase -
IEM32_RS27405 51260..51826 + 567 Protein_46 IS3-like element IS600 family transposase -
IEM32_RS27410 51870..52085 + 216 Protein_47 transposase -
IEM32_RS27415 52185..52391 - 207 WP_001105064.1 hypothetical protein -
IEM32_RS27420 52486..52761 - 276 WP_000421248.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IEM32_RS27425 52761..53045 - 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
IEM32_RS27430 53957..54814 - 858 WP_000130945.1 incFII family plasmid replication initiator RepA -
IEM32_RS27435 54807..54881 - 75 WP_001370046.1 RepA leader peptide Tap -
IEM32_RS27440 55117..55371 - 255 WP_000083831.1 replication regulatory protein RepA -
IEM32_RS27445 55611..55949 - 339 WP_000766796.1 hypothetical protein -
IEM32_RS27450 55987..56196 - 210 WP_001302200.1 hemolysin expression modulator Hha -
IEM32_RS27455 56242..56703 - 462 WP_001233853.1 thermonuclease family protein -
IEM32_RS27460 56948..57160 - 213 WP_001302189.1 ANR family transcriptional regulator -
IEM32_RS27465 57293..57853 - 561 WP_000840472.1 fertility inhibition protein FinO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hlyD / hlyB / hlyA / hlyC / exeG / exeE / stcE / espP / toxB / hlyD / hlyD / hlyD 1..104844 104844


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10735.42 Da        Isoelectric Point: 9.9324

>T173464 WP_000421248.1 NZ_CP062161:c52761-52486 [Escherichia coli]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T173464 NZ_CP080223:842824-843231 [Escherichia coli O141:H4]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT173464 WP_001178089.1 NZ_CP062161:c53045-52761 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT173464 NZ_CP080223:842577-842843 [Escherichia coli O141:H4]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z6FAZ0

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References