Detailed information of TA system TA173463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 5399..5924
• Replicon: plasmid punnamed1
• Accession: NZ_CP062161
• Organism: Escherichia coli strain 20R2R

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: IEM32_RS27200
• Protein ID: WP_001159868.1
• Coordinates: 5399..5704 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: IEM32_RS27205
• Protein ID: WP_000813634.1
• Coordinates: 5706..5924 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IEM32_RS27180	587..1768	-	1182	Protein_1	plasmid-partitioning protein SopA	-
IEM32_RS27185	2356..2694	-	339	WP_071525396.1	RepB family plasmid replication initiator protein	-
IEM32_RS27190	2656..3869	+	1214	WP_162829202.1	IS3 family transposase	-
IEM32_RS27195	4592..5398	-	807	WP_000016989.1	site-specific integrase	-
IEM32_RS27200	5399..5704	-	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
IEM32_RS27205	5706..5924	-	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
IEM32_RS27210	6383..7066	+	684	WP_010891291.1	DNA-binding protein	-
IEM32_RS27215	7063..7683	+	621	WP_001248529.1	hypothetical protein	-
IEM32_RS27220	7680..7880	+	201	WP_000708307.1	hypothetical protein	-
IEM32_RS27225	8288..9265	+	978	WP_000361615.1	RepB family plasmid replication initiator protein	-
IEM32_RS27230	9550..10290	-	741	WP_001066920.1	tyrosine-type recombinase/integrase	-
IEM32_RS27235	10411..10641	-	231	WP_010891288.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyD / hlyB / hlyA / hlyC / exeG / exeE / stcE / espP / toxB / hlyD / hlyD / hlyD	1..104844	104844

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T173463 WP_001159868.1 NZ_CP062161:c5704-5399 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T173463 NZ_CP080223:699906-700202 [Escherichia coli O141:H4]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT173463 WP_000813634.1 NZ_CP062161:c5924-5706 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT173463 NZ_CP080223:700204-700599 [Escherichia coli O141:H4]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2