T4SS ID	97
Strain	Escherichia coli plasmid pVM01 (NC_010409)
Replicon	plasmid pVM01 [Browse all T4SS(s) in this replicon]
Accession	NC_010409
Location	93994..127662
Name	Tra_F
Function	conjugation
Classification	Type IVA; Type F
This T4SS has been mentioned in a genome sequencing report.

T4SS components

Component	TraA_F	TraB_F	TraC_F	TraD_F	TraE_F	TraF_F	TraG_F	TraH_F	TraI_F
Number	1	1	1	1	1	1	1	1	1
Component	TraK_F	TraL_F	TraM_F	TraN_F	TraQ_F	TraU_F	TraV_F	TraW_F	TraX_F
Number	1	1	1	1	1	1	1	1	1
Component	TraY_F	TrbC_F	Orf169_F
Number	1	1	1

The information of T4SS components from NC_010409
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Component
1	pVM01_p080	89017..90150 [-], 1134	169546468	hypothetical protein
2	pVM01_p081	90256..90579 [+], 324	169546507	hypothetical protein
3	pVM01_p082	90875..91378 [+], 504	169546469	hypothetical protein
4	pVM01_p083	92412..92972 [-], 561	169546470	conjugal transfer fertility inhibition protein FinO
5	pVM01_p084	93075..93935 [-], 861	169546508	hypothetical protein
6	pVM01_p085	93994..94740 [-], 747	169546471	conjugal transfer pilus acetylation protein TraX	TraX_F
7	pVM01_p086	94760..100030 [-], 5271	169546472	conjugal transfer nickase/helicase TraI	TraI_F
8	pVM01_p087	100030..102183 [-], 2154	169546473	conjugal transfer protein TraD	TraD_F
9	pVM01_p088	102436..103167 [-], 732	169546474	conjugal transfer surface exclusion protein TraT
10	pVM01_p089	103181..103690 [-], 510	169546475	conjugal transfer entry exclusion protein TraS
11	pVM01_p090	103687..106512 [-], 2826	169546476	conjugal transfer mating pair stabilization protein TraG	TraG_F
12	pVM01_p091	106509..107882 [-], 1374	169546477	conjugal transfer pilus assembly protein TraH	TraH_F
13	pVM01_p092	108073..108261 [-], 189	169546509	hypothetical protein
14	pVM01_p093	108248..108529 [-], 282	169546478	conjugal transfer protein TrbJ
15	pVM01_p094	108519..109064 [-], 546	169546479	conjugal transfer protein TrbB
16	pVM01_p095	109051..109332 [-], 282	169546480	conjugal transfer pilin chaperone TraQ	TraQ_F
17	pVM01_p096	109448..110191 [-], 744	169546481	conjugal pilus assembly protein TraF	TraF_F
18	pVM01_p097	110184..110441 [-], 258	169546482	conjugal transfer protein TrbE
19	pVM01_p098	110468..112318 [-], 1851	169546483	conjugal transfer mating pair stabilization protein TraN	TraN_F
20	pVM01_p099	112315..112953 [-], 639	169546484	conjugal transfer pilus assembly protein TrbC	TrbC_F
21	pVM01_p100	112981..113433 [-], 453	169546510	hypothetical protein
22	pVM01_p101	113463..113924 [-], 462	169546511	hypothetical protein
23	pVM01_p102	113950..114942 [-], 993	169546512	conjugal transfer pilus assembly protein TraU	TraU_F
24	pVM01_p103	114939..115571 [-], 633	169546513	conjugal transfer pilus assembly protein TraW	TraW_F
25	pVM01_p104	115568..115954 [-], 387	169546514	conjugal transfer protein TrbI
26	pVM01_p105	115951..118581 [-], 2631	169546515	conjugal transfer ATP-binding protein TraC	TraC_F
27	pVM01_p106	118708..119055 [-], 348	169546516	hypothetical protein
28	pVM01_p107	119083..119301 [-], 219	169546517	hypothetical protein
29	pVM01_p108	119381..119854 [-], 474	169546518	hypothetical protein
30	pVM01_p109	119903..119986 [-], 84	169546519	hypothetical protein
31	pVM01_p110	120139..120654 [-], 516	169546520	conjugal transfer protein TraV	TraV_F
32	pVM01_p111	120651..120971 [-], 321	169546521	conjugal transfer protein TrbD
33	pVM01_p112	120958..121548 [-], 591	169546522	conjugal transfer protein TraP
34	pVM01_p113	121538..122965 [-], 1428	169546523	conjugal transfer pilus assembly protein TraB	TraB_F
35	pVM01_p114	122965..123693 [-], 729	169546524	conjugal transfer protein TraK	TraK_F
36	pVM01_p115	123680..124246 [-], 567	169546525	conjugal transfer pilus assembly protein TraE	TraE_F
37	pVM01_p116	124268..124579 [-], 312	169546526	conjugal transfer pilus assembly protein TraL	TraL_F
38	pVM01_p117	124594..124953 [-], 360	169546527	conjugal transfer pilin subunit TraA	TraA_F
39	pVM01_p118	124986..125345 [-], 360	169546528	conjugal transfer protein TraY	TraY_F
40	pVM01_p119	125480..126169 [-], 690	169546529	conjugal transfer transcriptional regulator TraJ
41	pVM01_p120	126356..126739 [-], 384	169546530	conjugal transfer protein TraM	TraM_F
42	pVM01_p121	127153..127662 [+], 510	169546531	lytic transglycosylase	Orf169_F
43	pVM01_p122	127959..128780 [-], 822	169546532	hypothetical protein
44	pVM01_p123	128900..129040 [-], 141	169546533	hypothetical protein
45	pVM01_p124	129075..129329 [-], 255	169546534	hypothetical protein
46	pVM01_p125	130321..130458 [-], 138	169546535	hypothetical protein
47	pVM01_p126	130458..131177 [-], 720	169546536	plasmid SOS inhibition protein A
48	pVM01_p127 (psiB)	131174..131608 [-], 435	169546537	plasmid SOS inhibition protein B

 This T4SS contains information of accessory protein.

Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files

Proteins        Genes

The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#	Accessory protein(GI)	motif(s)	Substrate(s)	Function	Reference
1	TraM (169546530)	ND	TraI	For transfer initiation, traM mediates signalling between the relaxosome and transferosome.	(1) PubMed: 22788760

Tips:
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Wong JJ; Lu J; Glover JN (2012). Relaxosome function and conjugation regulation in F-like plasmids - a structural biology perspective. Mol Microbiol. 85(4):602-17. [PudMed:22788760]

(1) Tivendale KA; Noormohammadi AH; Allen JL; Browning GF (2009). The conserved portion of the putative virulence region contributes to virulence of avian pathogenic Escherichia coli. Microbiology. 155(Pt 2):450-60. [PudMed:19202093]