StrainEscherichia coli plasmid pVM01 (NC_010409)
Repliconplasmid pVM01 [Browse all T4SS(s) in this replicon]
ClassificationType IVA; Type F
sequence This T4SS has been mentioned in a genome sequencing report.

T4SS components

The information of T4SS components from NC_010409
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1pVM01_p08089017..90150 [-], 1134169546468hypothetical protein 
2pVM01_p08190256..90579 [+], 324169546507hypothetical protein 
3pVM01_p08290875..91378 [+], 504169546469hypothetical protein 
4pVM01_p08392412..92972 [-], 561169546470conjugal transfer fertility inhibition protein FinO 
5pVM01_p08493075..93935 [-], 861169546508hypothetical protein 
6pVM01_p08593994..94740 [-], 747169546471conjugal transfer pilus acetylation protein TraX  TraX_F
7pVM01_p08694760..100030 [-], 5271169546472conjugal transfer nickase/helicase TraI  TraI_F
8pVM01_p087100030..102183 [-], 2154169546473conjugal transfer protein TraD  TraD_F
9pVM01_p088102436..103167 [-], 732169546474conjugal transfer surface exclusion protein TraT 
10pVM01_p089103181..103690 [-], 510169546475conjugal transfer entry exclusion protein TraS 
11pVM01_p090103687..106512 [-], 2826169546476conjugal transfer mating pair stabilization protein TraG  TraG_F
12pVM01_p091106509..107882 [-], 1374169546477conjugal transfer pilus assembly protein TraH  TraH_F
13pVM01_p092108073..108261 [-], 189169546509hypothetical protein 
14pVM01_p093108248..108529 [-], 282169546478conjugal transfer protein TrbJ 
15pVM01_p094108519..109064 [-], 546169546479conjugal transfer protein TrbB 
16pVM01_p095109051..109332 [-], 282169546480conjugal transfer pilin chaperone TraQ  TraQ_F
17pVM01_p096109448..110191 [-], 744169546481conjugal pilus assembly protein TraF  TraF_F
18pVM01_p097110184..110441 [-], 258169546482conjugal transfer protein TrbE 
19pVM01_p098110468..112318 [-], 1851169546483conjugal transfer mating pair stabilization protein TraN  TraN_F
20pVM01_p099112315..112953 [-], 639169546484conjugal transfer pilus assembly protein TrbC  TrbC_F
21pVM01_p100112981..113433 [-], 453169546510hypothetical protein 
22pVM01_p101113463..113924 [-], 462169546511hypothetical protein 
23pVM01_p102113950..114942 [-], 993169546512conjugal transfer pilus assembly protein TraU  TraU_F
24pVM01_p103114939..115571 [-], 633169546513conjugal transfer pilus assembly protein TraW  TraW_F
25pVM01_p104115568..115954 [-], 387169546514conjugal transfer protein TrbI 
26pVM01_p105115951..118581 [-], 2631169546515conjugal transfer ATP-binding protein TraC  TraC_F
27pVM01_p106118708..119055 [-], 348169546516hypothetical protein 
28pVM01_p107119083..119301 [-], 219169546517hypothetical protein 
29pVM01_p108119381..119854 [-], 474169546518hypothetical protein 
30pVM01_p109119903..119986 [-], 84169546519hypothetical protein 
31pVM01_p110120139..120654 [-], 516169546520conjugal transfer protein TraV  TraV_F
32pVM01_p111120651..120971 [-], 321169546521conjugal transfer protein TrbD 
33pVM01_p112120958..121548 [-], 591169546522conjugal transfer protein TraP 
34pVM01_p113121538..122965 [-], 1428169546523conjugal transfer pilus assembly protein TraB  TraB_F
35pVM01_p114122965..123693 [-], 729169546524conjugal transfer protein TraK  TraK_F
36pVM01_p115123680..124246 [-], 567169546525conjugal transfer pilus assembly protein TraE  TraE_F
37pVM01_p116124268..124579 [-], 312169546526conjugal transfer pilus assembly protein TraL  TraL_F
38pVM01_p117124594..124953 [-], 360169546527conjugal transfer pilin subunit TraA  TraA_F
39pVM01_p118124986..125345 [-], 360169546528conjugal transfer protein TraY  TraY_F
40pVM01_p119125480..126169 [-], 690169546529conjugal transfer transcriptional regulator TraJ 
41pVM01_p120126356..126739 [-], 384169546530conjugal transfer protein TraM  TraM_Faccesspry protein
42pVM01_p121127153..127662 [+], 510169546531lytic transglycosylase  Orf169_F
43pVM01_p122127959..128780 [-], 822169546532hypothetical protein 
44pVM01_p123128900..129040 [-], 141169546533hypothetical protein 
45pVM01_p124129075..129329 [-], 255169546534hypothetical protein 
46pVM01_p125130321..130458 [-], 138169546535hypothetical protein 
47pVM01_p126130458..131177 [-], 720169546536plasmid SOS inhibition protein A 
48pVM01_p127 (psiB)131174..131608 [-], 435169546537plasmid SOS inhibition protein B 
accesspry protein This T4SS contains information of accessory protein.
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

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Proteins        Genes
The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#Accessory protein(GI)motif(s)Substrate(s)FunctionReference
1TraM (169546530)NDTraIFor transfer initiation, traM mediates signalling between the relaxosome and transferosome.(1) PubMed: 22788760
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Wong JJ; Lu J; Glover JN (2012). Relaxosome function and conjugation regulation in F-like plasmids - a structural biology perspective. Mol Microbiol. 85(4):602-17. [PudMed:22788760]
(1) Tivendale KA; Noormohammadi AH; Allen JL; Browning GF (2009). The conserved portion of the putative virulence region contributes to virulence of avian pathogenic Escherichia coli. Microbiology. 155(Pt 2):450-60. [PudMed:19202093]