T4SS ID503
StrainAnaplasma phagocytophilum HZ
Repliconchromosome [Browse all T4SS(s) in this replicon]
Location86392..87207; 365015..384720; 431711..432943
Functioneffector translocation
ClassificationType IVA; Type P
experimental Experimental investigation has been performed on this T4SS.

T4SS components

The information of T4SS components from NC_007797
Region 1: 86392..87207
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1APH_007582103..82651 [+], 54988607622putative methyltransferase 
2APH_007783993..84916 [-], 92488607780auxin efflux carrier family protein 
3APH_007885022..85330 [-], 30988607239putative oxidoreductase 
4APH_0079 (xseB)85386..85613 [+], 22888607825exodeoxyribonuclease VII, small subunit 
5APH_0080 (trx)86047..86379 [-], 33388607814thioredoxin 
6APH_0081 (virB9-1)86392..87207 [-], 81688607651type IV secretion system protein VirB9  VirB9
7APH_0082 (pdhA)87566..88603 [-], 103888607434pyruvate dehydrogenase complex, E1 component, pyruvate dehydrogenase alpha subunit 
8APH_008388626..89504 [-], 87988607297alpha/beta fold family hydrolase 
Region 2: 365015..384720
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1APH_0367 (lipA)360960..361847 [+], 88888607495lipoyl synthase 
2APH_0369362127..362219 [+], 9388606988hypothetical protein 
3APH_0370 (pstA)362609..363955 [-], 134788607119phosphate ABC transporter, permease protein PtsA 
4APH_0371 (sodB)364302..364922 [+], 62188606849Fe superoxide dismutase 
5APH_0372 (virB3)365015..365308 [+], 29488607882type IV secretion system protein VirB3  VirB3
6APH_0373 (virB4-1)365309..367714 [+], 240688607176type IV secretion system ATPase VirB4  VirB4
7APH_0374 (virB6)367707..370247 [+], 254188607323type IV secretion system protein VirB6  VirB6
8APH_0375370385..373171 [+], 278788607745VirB6 family type IV secretion system protein  VirB6
9APH_0376373184..377617 [+], 443488607035type IV secretion systemprotein VirB6 family  VirB6
10APH_0377377638..384720 [+], 708388607561type IV secretion system VirB6 family protein  VirB6
11APH_0378384798..385406 [-], 60988607365hypothetical protein 
12APH_0379 (dut)386447..386887 [-], 44188606839deoxyuridine 5'-triphosphate nucleotidohydrolase 
13APH_0380 (ispH)387407..388381 [-], 975886071794-hydroxy-3-methylbut-2-enyl diphosphate reductase 
Region 3: 431711..432943
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1APH_0409426766..426903 [+], 13888607524hypothetical protein 
2APH_0411 (mutM)427134..427940 [+], 80788606811formamidopyrimidine-DNA glycosylase 
3APH_0413429245..429418 [+], 17488607741hypothetical protein 
4APH_0414429456..429617 [-], 16288607827hypothetical protein 
5APH_0415 (pccB)430072..431601 [+], 153088607086propionyl-CoA carboxylase, beta subunit 
6APH_0416431627..431722 [+], 9688607275hypothetical protein 
7APH_0417431711..431842 [-], 13288606719type IV secretion system protein VirB4, N-terminal truncation  VirB4
8APH_0418431977..432117 [-], 14188607552type IV secretion system protein VirB6,C-terminal truncation 
9APH_0419432110..432943 [-], 83488606981type IV secretion system protein VirB4, truncation  VirB4
10APH_0420 (prfA)433514..434593 [-], 108088606704peptide chain release factor 1 
11APH_0421 (engB)434919..435500 [+], 58288607557ribosome biogenesis GTP-binding protein YsxC 
12APH_0422 (argB)435478..436461 [+], 98488607288acetylglutamate kinase 
13APH_0423 (hemF)436644..437468 [+], 82588607423coproporphyrinogen III oxidase 
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files
Proteins        Genes

Ats-1, APH0215, HGE-14 protein, AnkA

The information of protein effectors
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProduct  *
1APH_0859 912788..913549 [+], 76288606850hypothetical proteinexperimental  Ats-1interaction
2APH_0215 214429..214863 [-], 43588607504hypothetical proteinexperimental  APH0215
3APH_0455 483333..485846 [+], 251488607661HGE-14 proteinexperimental  HGE-14 protein
4APH_0740 (ank)784800..788498 [-], 369988607707ankyrinexperimental  AnkAinteraction
experimental T4SE derived from experimental literature.
interaction This effector contains information of interaction.

Download FASTA format files
Proteins        Genes
The information on host-pathogen interaction.

#Name(Protein GI)Host site/SubstrateSourceFunctionReference
1AnkA (88607707)HDAC1humanAnkA interact with HDAC1 directly at the CYBB(the NADPH oxidase component) promoter to make CYBB down-regulated.(1) PubMed: 25996657experimental
2AnkA (88607707)SHP-1humanAnkA is phosphorylated and then binds to the host protein Src homology phosphatase-1 (SHP-1) to manipulate the host cell.(2) PubMed: 17250594insolico
3Ats-1 (88606850)BECN1murineBECN1 is a protein critical for autophagy nucleation. Ats-1 interaction with BECN1 and induces formation of autophagosome to overturn autophagic machinery of host cell to promote infection.(3) PubMed: 23388398experimental
experimental This T4SE is mentioned in the literature.
insolico This T4SE is highly identical with the protein mentioned in the literature.
(1) Rennoll-Bankert KE et al. (2015). Chromatin-bound bacterial effector ankyrin A recruits histone deacetylase 1 and modifies host gene expression. Cell Microbiol. 17(11):1640-52. [PudMed:25996657] experimental
(2) IJdo JW et al. (2007). Anaplasma phagocytophilum AnkA is tyrosine-phosphorylated at EPIYA motifs and recruits SHP-1 during early infection. Cell Microbiol. 9(5):1284-96. [PudMed:17250594] experimental
(3) Niu H et al. (2013). Ats-1: a novel bacterial molecule that links autophagy to bacterial nutrition. Autophagy. 9(5):787-8. [PudMed:23388398] experimental
(1) Rikihisa Y; Lin M; Niu H (2010). Type IV secretion in the obligatory intracellular bacterium Anaplasma phagocytophilum. Cell Microbiol. 12(9):1213-21. [PudMed:20670295] experimental
(2) Niu H; Kozjak-Pavlovic V; Rudel T; Rikihisa Y (2010). Anaplasma phagocytophilum Ats-1 is imported into host cell mitochondria and interferes with apoptosis induction. PLoS Pathog. 6(2):e1000774. [PudMed:20174550] experimental
(3) Nelson CM; Herron MJ; Felsheim RF; Schloeder BR; Grindle SM; Chavez AO; Kurtti TJ; Munderloh UG (2008). Whole genome transcription profiling of Anaplasma phagocytophilum in human and tick host cells by tiling array analysis. BMC Genomics. 9:364. [PudMed:18671858] experimental
(4) Lin M; den Dulk-Ras A; Hooykaas PJ; Rikihisa Y (2007). Anaplasma phagocytophilum AnkA secreted by type IV secretion system is tyrosine phosphorylated by Abl-1 to facilitate infection. Cell Microbiol. 9(11):2644-57. [PudMed:17587335] experimental
(5) Dunning Hotopp JC; Lin M; Madupu R; Crabtree J; Angiuoli SV; Eisen JA; Seshadri R; Ren Q; Wu M; Utterback TR; Smith S; Lewis M; Khouri H; Zhang C; Niu H; Lin Q; Ohashi N; Zhi N; Nelson W; Brinkac LM; Dodson RJ; Rosovitz MJ; Sundaram J; Daugherty SC; Davidsen T; Durkin AS; Gwinn M; Haft DH; Selengut JD; Sullivan SA; Zafar N; Zhou L; Benahmed F; Forberger H; Halpin R; Mulligan S; Robinson J; White O; Rikihisa Y; Tettelin H (2006). Comparative genomics of emerging human ehrlichiosis agents. PLoS Genet. 2(2):e21. [PudMed:16482227]
(6) Niu H; Rikihisa Y; Yamaguchi M; Ohashi N (2006). Differential expression of VirB9 and VirB6 during the life cycle of Anaplasma phagocytophilum in human leucocytes is associated with differential binding and avoidance of lysosome pathway. Cell Microbiol. 8(3):523-34. [PudMed:16469062] experimental
(7) Ohashi N; Zhi N; Lin Q; Rikihisa Y (2002). Characterization and transcriptional analysis of gene clusters for a type IV secretion machinery in human granulocytic and monocytic ehrlichiosis agents. Infect Immun. 70(4):2128-38. [PudMed:11895979] experimental
experimental This literature contains experimental investigation