T4SS ID	503
Strain	Anaplasma phagocytophilum HZ
Replicon	chromosome [Browse all T4SS(s) in this replicon]
Accession	NC_007797
Location	86392..87207; 365015..384720; 431711..432943
Name	VirB
Function	effector translocation
Classification	Type IVA; Type P
Experimental investigation has been performed on this T4SS.

T4SS components

Component	VirB1	VirB2	VirB3	VirB4	VirB5	VirB6	VirB7	VirB8	VirB9
Number	0	8	1	4	0	4	0	1	2
Component	VirB10	VirB11	VirD4
Number	1	1	1

The information of T4SS components from NC_007797
Region 1: 86392..87207
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Component
1	APH_0075	82103..82651 [+], 549	88607622	putative methyltransferase
2	APH_0077	83993..84916 [-], 924	88607780	auxin efflux carrier family protein
3	APH_0078	85022..85330 [-], 309	88607239	putative oxidoreductase
4	APH_0079 (xseB)	85386..85613 [+], 228	88607825	exodeoxyribonuclease VII, small subunit
5	APH_0080 (trx)	86047..86379 [-], 333	88607814	thioredoxin
6	APH_0081 (virB9-1)	86392..87207 [-], 816	88607651	type IV secretion system protein VirB9	VirB9
7	APH_0082 (pdhA)	87566..88603 [-], 1038	88607434	pyruvate dehydrogenase complex, E1 component, pyruvate dehydrogenase alpha subunit
8	APH_0083	88626..89504 [-], 879	88607297	alpha/beta fold family hydrolase
Region 2: 365015..384720
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Component
1	APH_0367 (lipA)	360960..361847 [+], 888	88607495	lipoyl synthase
2	APH_0369	362127..362219 [+], 93	88606988	hypothetical protein
3	APH_0370 (pstA)	362609..363955 [-], 1347	88607119	phosphate ABC transporter, permease protein PtsA
4	APH_0371 (sodB)	364302..364922 [+], 621	88606849	Fe superoxide dismutase
5	APH_0372 (virB3)	365015..365308 [+], 294	88607882	type IV secretion system protein VirB3	VirB3
6	APH_0373 (virB4-1)	365309..367714 [+], 2406	88607176	type IV secretion system ATPase VirB4	VirB4
7	APH_0374 (virB6)	367707..370247 [+], 2541	88607323	type IV secretion system protein VirB6	VirB6
8	APH_0375	370385..373171 [+], 2787	88607745	VirB6 family type IV secretion system protein	VirB6
9	APH_0376	373184..377617 [+], 4434	88607035	type IV secretion systemprotein VirB6 family	VirB6
10	APH_0377	377638..384720 [+], 7083	88607561	type IV secretion system VirB6 family protein	VirB6
11	APH_0378	384798..385406 [-], 609	88607365	hypothetical protein
12	APH_0379 (dut)	386447..386887 [-], 441	88606839	deoxyuridine 5'-triphosphate nucleotidohydrolase
13	APH_0380 (ispH)	387407..388381 [-], 975	88607179	4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Region 3: 431711..432943
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Component
1	APH_0409	426766..426903 [+], 138	88607524	hypothetical protein
2	APH_0411 (mutM)	427134..427940 [+], 807	88606811	formamidopyrimidine-DNA glycosylase
3	APH_0413	429245..429418 [+], 174	88607741	hypothetical protein
4	APH_0414	429456..429617 [-], 162	88607827	hypothetical protein
5	APH_0415 (pccB)	430072..431601 [+], 1530	88607086	propionyl-CoA carboxylase, beta subunit
6	APH_0416	431627..431722 [+], 96	88607275	hypothetical protein
7	APH_0417	431711..431842 [-], 132	88606719	type IV secretion system protein VirB4, N-terminal truncation	VirB4
8	APH_0418	431977..432117 [-], 141	88607552	type IV secretion system protein VirB6,C-terminal truncation
9	APH_0419	432110..432943 [-], 834	88606981	type IV secretion system protein VirB4, truncation	VirB4
10	APH_0420 (prfA)	433514..434593 [-], 1080	88606704	peptide chain release factor 1
11	APH_0421 (engB)	434919..435500 [+], 582	88607557	ribosome biogenesis GTP-binding protein YsxC
12	APH_0422 (argB)	435478..436461 [+], 984	88607288	acetylglutamate kinase
13	APH_0423 (hemF)	436644..437468 [+], 825	88607423	coproporphyrinogen III oxidase

Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files

Proteins        Genes

Effectors

Ats-1, APH0215, HGE-14 protein, AnkA

The information of protein effectors
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	*
1	APH_0859	912788..913549 [+], 762	88606850	hypothetical protein	Ats-1
2	APH_0215	214429..214863 [-], 435	88607504	hypothetical protein	APH0215
3	APH_0455	483333..485846 [+], 2514	88607661	HGE-14 protein	HGE-14 protein
4	APH_0740 (ank)	784800..788498 [-], 3699	88607707	ankyrin	AnkA

 T4SE derived from experimental literature.
 This effector contains information of interaction.

Download FASTA format files

Proteins        Genes

The information on host-pathogen interaction.

#	Name(Protein GI)	Host site/Substrate	Source	Function	Reference
1	AnkA (88607707)	HDAC1	human	AnkA interact with HDAC1 directly at the CYBB(the NADPH oxidase component) promoter to make CYBB down-regulated.	(1) PubMed: 25996657
2	AnkA (88607707)	SHP-1	human	AnkA is phosphorylated and then binds to the host protein Src homology phosphatase-1 (SHP-1) to manipulate the host cell.	(2) PubMed: 17250594
3	Ats-1 (88606850)	BECN1	murine	BECN1 is a protein critical for autophagy nucleation. Ats-1 interaction with BECN1 and induces formation of autophagosome to overturn autophagic machinery of host cell to promote infection.	(3) PubMed: 23388398

This T4SE is mentioned in the literature.
This T4SE is highly identical with the protein mentioned in the literature.

(1) Rennoll-Bankert KE et al. (2015). Chromatin-bound bacterial effector ankyrin A recruits histone deacetylase 1 and modifies host gene expression. Cell Microbiol. 17(11):1640-52. [PudMed:25996657]

(2) IJdo JW et al. (2007). Anaplasma phagocytophilum AnkA is tyrosine-phosphorylated at EPIYA motifs and recruits SHP-1 during early infection. Cell Microbiol. 9(5):1284-96. [PudMed:17250594]

(3) Niu H et al. (2013). Ats-1: a novel bacterial molecule that links autophagy to bacterial nutrition. Autophagy. 9(5):787-8. [PudMed:23388398]

(1) Rikihisa Y; Lin M; Niu H (2010). Type IV secretion in the obligatory intracellular bacterium Anaplasma phagocytophilum. Cell Microbiol. 12(9):1213-21. [PudMed:20670295]

(2) Niu H; Kozjak-Pavlovic V; Rudel T; Rikihisa Y (2010). Anaplasma phagocytophilum Ats-1 is imported into host cell mitochondria and interferes with apoptosis induction. PLoS Pathog. 6(2):e1000774. [PudMed:20174550]

(3) Nelson CM; Herron MJ; Felsheim RF; Schloeder BR; Grindle SM; Chavez AO; Kurtti TJ; Munderloh UG (2008). Whole genome transcription profiling of Anaplasma phagocytophilum in human and tick host cells by tiling array analysis. BMC Genomics. 9:364. [PudMed:18671858]

(4) Lin M; den Dulk-Ras A; Hooykaas PJ; Rikihisa Y (2007). Anaplasma phagocytophilum AnkA secreted by type IV secretion system is tyrosine phosphorylated by Abl-1 to facilitate infection. Cell Microbiol. 9(11):2644-57. [PudMed:17587335]

(5) Dunning Hotopp JC; Lin M; Madupu R; Crabtree J; Angiuoli SV; Eisen JA; Seshadri R; Ren Q; Wu M; Utterback TR; Smith S; Lewis M; Khouri H; Zhang C; Niu H; Lin Q; Ohashi N; Zhi N; Nelson W; Brinkac LM; Dodson RJ; Rosovitz MJ; Sundaram J; Daugherty SC; Davidsen T; Durkin AS; Gwinn M; Haft DH; Selengut JD; Sullivan SA; Zafar N; Zhou L; Benahmed F; Forberger H; Halpin R; Mulligan S; Robinson J; White O; Rikihisa Y; Tettelin H (2006). Comparative genomics of emerging human ehrlichiosis agents. PLoS Genet. 2(2):e21. [PudMed:16482227]

(6) Niu H; Rikihisa Y; Yamaguchi M; Ohashi N (2006). Differential expression of VirB9 and VirB6 during the life cycle of Anaplasma phagocytophilum in human leucocytes is associated with differential binding and avoidance of lysosome pathway. Cell Microbiol. 8(3):523-34. [PudMed:16469062]

(7) Ohashi N; Zhi N; Lin Q; Rikihisa Y (2002). Characterization and transcriptional analysis of gene clusters for a type IV secretion machinery in human granulocytic and monocytic ehrlichiosis agents. Infect Immun. 70(4):2128-38. [PudMed:11895979]

This literature contains experimental investigation