Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QMN13_RS11340 Genome accession   NZ_AP027203
Coordinates   2269252..2270151 (+) Length   299 a.a.
NCBI ID   WP_281970227.1    Uniprot ID   -
Organism   Polynucleobacter sp. SHI2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2264252..2275151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMN13_RS11310 (PSHI2_22140) pth 2264436..2265026 (+) 591 WP_281970221.1 aminoacyl-tRNA hydrolase -
  QMN13_RS11315 (PSHI2_22150) - 2265073..2265321 (-) 249 WP_281970222.1 YfhL family 4Fe-4S dicluster ferredoxin -
  QMN13_RS11320 (PSHI2_22160) coaD 2265325..2265813 (-) 489 WP_281970223.1 pantetheine-phosphate adenylyltransferase -
  QMN13_RS11325 (PSHI2_22170) rsmD 2265841..2266446 (-) 606 WP_281970224.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  QMN13_RS11330 (PSHI2_22180) - 2266443..2267783 (-) 1341 WP_281970225.1 pitrilysin family protein -
  QMN13_RS11335 (PSHI2_22190) - 2267795..2269129 (-) 1335 WP_281970226.1 pitrilysin family protein -
  QMN13_RS11340 (PSHI2_22200) pilA 2269252..2270151 (+) 900 WP_281970227.1 signal recognition particle-docking protein FtsY Machinery gene
  QMN13_RS11345 (PSHI2_22210) - 2270382..2271686 (+) 1305 WP_281970228.1 AAA family ATPase -
  QMN13_RS11350 - 2272174..2274039 (+) 1866 WP_281994006.1 response regulator -
  QMN13_RS11355 (PSHI2_22240) rpoH 2274253..2275104 (+) 852 WP_281971783.1 RNA polymerase sigma factor RpoH -

Sequence

Protein


Download         Length: 299 a.a.        Molecular weight: 32566.59 Da        Isoelectric Point: 6.9164

>NTDB_id=99995 QMN13_RS11340 WP_281970227.1 2269252..2270151(+) (pilA) [Polynucleobacter sp. SHI2]
MFGLRKTLSSLFNRSKVDEELFESLEESLLLADVGIHATQELMNKLRSTSKKEKISDPIELKKQLEILIAELLYPLEFNI
NPLNQHTPTHTPEIWLIVGVNGAGKTTTIGKLCHALQNQNKKVLLAAGDTFRAAAKEQLGEWGKRNEVEVITQSSGDAAT
VAHDAIGAALARQADILIIDTAGRLATQSNLMEELKKVKRVIQKLIPSAPHQTLLVLDGNTGQNGLTQVKAFHEALQLSS
LIITKVDGTAKGGVLCAIAHSLRSTPNFPQIMALGVGEKMEQIQAFSALLFAQELLNND

Nucleotide


Download         Length: 900 bp        

>NTDB_id=99995 QMN13_RS11340 WP_281970227.1 2269252..2270151(+) (pilA) [Polynucleobacter sp. SHI2]
ATGTTTGGTTTACGCAAAACTTTATCATCCCTATTTAACCGCTCTAAGGTTGATGAAGAATTATTTGAATCTCTCGAAGA
GTCATTGTTATTAGCTGATGTGGGCATTCATGCCACACAAGAACTAATGAACAAATTACGCTCCACTTCTAAAAAAGAAA
AAATATCGGATCCTATTGAACTCAAAAAACAATTAGAAATTCTTATTGCAGAACTTCTCTATCCCTTAGAGTTTAATATT
AATCCTTTAAATCAGCATACCCCTACTCACACCCCTGAAATCTGGCTCATTGTAGGTGTTAATGGCGCAGGTAAAACCAC
AACTATAGGCAAATTGTGCCACGCACTCCAAAATCAAAATAAAAAGGTCTTATTGGCGGCTGGAGATACGTTTCGAGCTG
CCGCAAAGGAACAGTTAGGAGAATGGGGTAAGCGCAATGAGGTTGAAGTCATTACTCAATCTAGTGGAGATGCTGCCACA
GTTGCTCATGATGCGATTGGTGCTGCACTTGCAAGACAAGCCGACATATTGATTATTGATACTGCAGGTCGACTTGCAAC
TCAATCGAATTTGATGGAAGAACTCAAAAAGGTCAAAAGGGTTATCCAAAAATTAATCCCTAGTGCTCCACACCAAACTC
TTTTGGTATTAGATGGCAATACTGGCCAAAATGGCTTAACGCAGGTTAAAGCATTTCATGAAGCTTTACAACTATCTAGT
TTGATTATCACAAAGGTTGATGGTACAGCTAAAGGGGGGGTTCTATGTGCAATCGCTCACTCTTTGCGTTCTACCCCAAA
TTTCCCTCAAATTATGGCTCTTGGAGTAGGAGAAAAAATGGAACAAATTCAGGCATTTTCAGCGCTTTTATTTGCACAAG
AGTTGTTAAACAACGATTAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(3-64)

SRP54-type protein, GTPase domain (SRP54)

(93-296)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

49.32

98.328

0.485

Multiple sequence alignment