Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AAID08_RS00745 Genome accession   NZ_CP155917
Coordinates   165928..167373 (+) Length   481 a.a.
NCBI ID   WP_054504384.1    Uniprot ID   -
Organism   Achromobacter sp. EB05     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 160928..172373
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAID08_RS00730 (AAID08_00725) leuA 162009..163718 (-) 1710 WP_054504382.1 2-isopropylmalate synthase -
  AAID08_RS00735 (AAID08_00730) - 164110..165087 (-) 978 WP_064522604.1 tripartite tricarboxylate transporter substrate binding protein -
  AAID08_RS00740 (AAID08_00735) - 165282..165929 (-) 648 WP_054504383.1 hypothetical protein -
  AAID08_RS00745 (AAID08_00740) rpoN/rpoN1 165928..167373 (+) 1446 WP_054504384.1 RNA polymerase factor sigma-54 Machinery gene
  AAID08_RS00750 (AAID08_00745) - 167493..167774 (+) 282 WP_049070497.1 bacterioferritin-associated ferredoxin -
  AAID08_RS00755 (AAID08_00750) - 167744..168664 (-) 921 WP_064522601.1 LysR substrate-binding domain-containing protein -
  AAID08_RS00760 (AAID08_00755) - 168778..169692 (+) 915 WP_054504386.1 DMT family transporter -
  AAID08_RS00765 (AAID08_00760) bfr 170117..170593 (+) 477 WP_006386993.1 bacterioferritin -
  AAID08_RS00770 (AAID08_00765) - 170678..171364 (-) 687 WP_006386994.1 hypothetical protein -

Sequence

Protein


Download         Length: 481 a.a.        Molecular weight: 52526.93 Da        Isoelectric Point: 4.8032

>NTDB_id=999663 AAID08_RS00745 WP_054504384.1 165928..167373(+) (rpoN/rpoN1) [Achromobacter sp. EB05]
MQNIVPMTRPILELRPGQHLTLTPQLQQSIRLLQLSTLDLEAEISQVLADNPLLERDDEPSSAETQAESERESSVQDDEP
APERDTPVEEMPGSGGVYPDDDSSLPEAARPDTLREHLLGQLVLTRAAPRDAALAALLIDELDENGYLGSPLEEILGWLP
AEMEPDLDELRAALSLLQSFDPPGIGARDMSDCLLLQLRNPDLTRLPEAADPKALACARLICAQHLALLATGNNARLCAA
VGCDEATLRVAHALILRLEPRPGRAWTVPAADYAVPDVIVRKTRRGWQVTLNSAAVPRLQINGLYAQMLGNQKESAHAGL
QAQLQQAKWMIRNVEQRFDTILRVAQAIVDRQTAFFSQGPAAMRPLILKDIAGELGLHESTISRATTQKYMLTPFGTLEL
KRFFGAGVSTDGGDATSATAVQTFIRQMVAEENRAKPLSDSQIMEKLADQGIVIARRTVAKYREALRIAPAALRKAQAAA
R

Nucleotide


Download         Length: 1446 bp        

>NTDB_id=999663 AAID08_RS00745 WP_054504384.1 165928..167373(+) (rpoN/rpoN1) [Achromobacter sp. EB05]
ATGCAAAATATTGTACCTATGACCCGTCCTATTTTAGAACTGCGGCCCGGCCAGCATCTGACGCTGACCCCTCAACTGCA
GCAATCCATCCGGTTGCTGCAGCTGTCCACGCTCGACCTCGAGGCGGAAATCTCGCAGGTGCTCGCCGATAATCCGTTGC
TGGAACGCGACGATGAACCCTCATCGGCCGAAACGCAGGCCGAATCCGAGCGTGAATCGTCGGTGCAGGACGACGAGCCC
GCGCCCGAACGCGACACGCCGGTGGAGGAAATGCCGGGTTCGGGCGGGGTCTACCCCGATGACGATTCCAGCCTCCCCGA
GGCCGCCCGGCCCGATACCCTGCGCGAACACCTGCTGGGCCAACTGGTGCTGACGCGCGCGGCGCCGCGCGATGCCGCGC
TGGCCGCGTTGCTGATCGACGAATTGGACGAGAACGGCTATCTGGGCTCGCCGCTGGAGGAAATCCTGGGCTGGCTGCCC
GCCGAGATGGAGCCGGACCTGGATGAACTGCGCGCCGCGCTGTCGCTGCTGCAGTCGTTCGATCCGCCCGGCATCGGCGC
CCGCGACATGTCCGACTGCCTCTTGTTGCAACTGCGCAACCCGGACCTGACGCGGCTGCCGGAGGCCGCCGACCCGAAGG
CGCTGGCTTGCGCCCGTCTCATTTGCGCCCAGCATCTGGCGTTGCTGGCCACCGGCAACAATGCCCGCCTGTGCGCGGCG
GTCGGTTGCGACGAGGCGACCCTGCGCGTCGCCCACGCCCTGATCCTGCGGCTTGAGCCGCGGCCCGGCCGCGCCTGGAC
GGTGCCGGCGGCCGATTACGCCGTGCCCGACGTGATCGTGCGCAAGACCCGTCGCGGCTGGCAGGTGACGCTGAACAGCG
CCGCCGTGCCGCGCCTGCAGATCAACGGTTTGTACGCGCAGATGTTGGGCAACCAGAAGGAATCGGCCCACGCCGGCCTG
CAGGCGCAACTGCAGCAGGCCAAGTGGATGATCCGCAACGTCGAACAGCGCTTCGACACGATCCTGCGGGTGGCCCAGGC
CATCGTGGACCGTCAGACCGCCTTCTTCAGCCAGGGGCCGGCGGCCATGCGGCCGCTCATCCTGAAGGACATCGCGGGCG
AACTGGGCCTGCACGAATCCACGATTTCGCGCGCCACGACGCAGAAGTACATGCTCACTCCGTTTGGCACGCTGGAGCTC
AAGCGCTTCTTCGGGGCGGGGGTGTCGACCGACGGCGGCGACGCCACTTCGGCCACGGCGGTGCAGACCTTCATCCGCCA
GATGGTGGCCGAGGAGAACCGCGCCAAGCCGTTGTCGGACAGCCAGATCATGGAAAAGCTGGCCGACCAGGGCATCGTCA
TCGCCCGTCGTACGGTCGCGAAATACCGCGAAGCGCTGCGGATCGCGCCGGCCGCGCTGCGCAAAGCGCAGGCCGCGGCG
CGATGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(320-475)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(108-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

48.911

100

0.514

Multiple sequence alignment