Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ABEG41_RS12970 Genome accession   NZ_CP155795
Coordinates   2506272..2506682 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain D137     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2501272..2511682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABEG41_RS12940 (ABEG41_12940) - 2501359..2501499 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  ABEG41_RS12945 (ABEG41_12945) yqeF 2501805..2502536 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  ABEG41_RS12950 (ABEG41_12950) cwlH 2502788..2503540 (-) 753 WP_032722147.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  ABEG41_RS12955 (ABEG41_12955) yqeD 2503727..2504353 (+) 627 WP_015714297.1 TVP38/TMEM64 family protein -
  ABEG41_RS12960 (ABEG41_12960) gnd 2504372..2505265 (-) 894 WP_347002665.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  ABEG41_RS12965 (ABEG41_12965) yqeB 2505517..2506239 (+) 723 WP_010886572.1 hypothetical protein -
  ABEG41_RS12970 (ABEG41_12970) nucA/comI 2506272..2506682 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  ABEG41_RS12975 (ABEG41_12975) - 2506878..2507225 (+) 348 Protein_2506 sigma-70 family RNA polymerase sigma factor -
  ABEG41_RS12980 (ABEG41_12980) spoIVCA 2507256..2508716 (-) 1461 WP_243737903.1 site-specific DNA recombinase SpoIVCA -
  ABEG41_RS12985 (ABEG41_12985) - 2508674..2508852 (-) 179 Protein_2508 hypothetical protein -
  ABEG41_RS12990 (ABEG41_12990) arsC 2509207..2509626 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  ABEG41_RS12995 (ABEG41_12995) acr3 2509638..2510678 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  ABEG41_RS13000 (ABEG41_13000) yqcK 2510701..2511141 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  ABEG41_RS13005 (ABEG41_13005) arsR 2511202..2511519 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=999405 ABEG41_RS12970 WP_009967785.1 2506272..2506682(-) (nucA/comI) [Bacillus subtilis strain D137]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=999405 ABEG41_RS12970 WP_009967785.1 2506272..2506682(-) (nucA/comI) [Bacillus subtilis strain D137]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTACCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment