Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   EL118_RS09225 Genome accession   NZ_LR134228
Coordinates   1920654..1922081 (+) Length   475 a.a.
NCBI ID   WP_000980570.1    Uniprot ID   -
Organism   Escherichia coli strain NCTC9699     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1915654..1927081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL118_RS09185 (NCTC9699_01838) yeeW 1915838..1916032 (+) 195 WP_000988599.1 DUF5983 family protein -
  EL118_RS09190 - 1916078..1916158 (+) 81 Protein_1792 hypothetical protein -
  EL118_RS09195 - 1916447..1916593 (-) 147 Protein_1793 transposase domain-containing protein -
  EL118_RS09200 - 1916647..1916829 (+) 183 WP_001016348.1 EutP/PduV family microcompartment system protein -
  EL118_RS09205 (NCTC9699_01839) tmaR 1916930..1917259 (-) 330 WP_000450409.1 DUF496 family protein -
  EL118_RS09210 (NCTC9699_01840) yeeA 1917431..1918489 (-) 1059 WP_001200905.1 FUSC family protein -
  EL118_RS09215 (NCTC9699_01841) sbmC 1918687..1919160 (-) 474 WP_001105393.1 DNA gyrase inhibitor SbmC -
  EL118_RS09220 (NCTC9699_01842) dacD 1919279..1920445 (-) 1167 WP_001320295.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  EL118_RS09225 (NCTC9699_01843) sbcB 1920654..1922081 (+) 1428 WP_000980570.1 exodeoxyribonuclease I Machinery gene
  EL118_RS09230 (NCTC9699_01844) tsuB 1922124..1922351 (-) 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  EL118_RS09235 (NCTC9699_01845) tsuA 1922365..1923423 (-) 1059 WP_000492340.1 thiosulfate utilization transporter TsuA/YeeE -
  EL118_RS24515 - 1924644..1924832 (+) 189 Protein_1803 IS4 family transposase -
  EL118_RS24520 - 1924811..1924984 (+) 174 Protein_1804 cobalamin biosynthesis protein -
  EL118_RS09255 (NCTC9699_01847) cobU 1925136..1925681 (+) 546 WP_000973176.1 bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase -
  EL118_RS09260 (NCTC9699_01848) cobS 1925678..1926421 (+) 744 WP_001326708.1 adenosylcobinamide-GDP ribazoletransferase -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54529.81 Da        Isoelectric Point: 5.4720

>NTDB_id=998723 EL118_RS09225 WP_000980570.1 1920654..1922081(+) (sbcB) [Escherichia coli strain NCTC9699]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EATFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=998723 EL118_RS09225 WP_000980570.1 1920654..1922081(+) (sbcB) [Escherichia coli strain NCTC9699]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGAACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACTGATAACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAAC
GAAGCCACGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGC
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCAGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTAAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTG
ACGCCGATCGCGCAGCAATGAAAATTGTGCTGGAAACCGAGCCACGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGAATTGAAAAACTGTTGTTCAATTATCGGGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGCCGCCAGGTATTCACACCCGAGTTTTTACAAGGCTATGCAGAAGAGATTCAGATGCTGGCGCAGCAGT
ATGCCGACGATAAAGAAAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTAG

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(13-188)

Exonuclease C-terminal (ExoI_SH3)

(212-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.723

98.947

0.581

Multiple sequence alignment