Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   ABDZ24_RS25070 Genome accession   NZ_CP155623
Coordinates   4775841..4779566 (-) Length   1241 a.a.
NCBI ID   WP_000572309.1    Uniprot ID   -
Organism   Bacillus paramobilis strain N24509     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4770841..4784566
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABDZ24_RS25020 (ABDZ24_25095) - 4771767..4772051 (+) 285 WP_076535967.1 hypothetical protein -
  ABDZ24_RS25025 (ABDZ24_25100) - 4772085..4772984 (-) 900 WP_001213066.1 fumarylacetoacetate hydrolase family protein -
  ABDZ24_RS25030 (ABDZ24_25105) - 4773231..4773410 (+) 180 WP_098258255.1 aspartyl-phosphate phosphatase Spo0E family protein -
  ABDZ24_RS25035 (ABDZ24_25110) gerPA 4773507..4773728 (+) 222 WP_001111188.1 spore germination protein GerPA -
  ABDZ24_RS25040 (ABDZ24_25115) gerPB 4773743..4773949 (+) 207 WP_001012508.1 spore germination protein GerPB -
  ABDZ24_RS25045 (ABDZ24_25120) gerPC 4774018..4774632 (+) 615 WP_001070752.1 spore germination protein GerPC -
  ABDZ24_RS25050 (ABDZ24_25125) gerPD 4774639..4774833 (+) 195 WP_001102342.1 spore germination protein GerPD -
  ABDZ24_RS25055 (ABDZ24_25130) - 4774849..4775235 (+) 387 WP_000902318.1 spore germination protein GerPE -
  ABDZ24_RS25060 (ABDZ24_25135) gerPF 4775278..4775493 (+) 216 WP_001141566.1 spore germination protein GerPF -
  ABDZ24_RS25065 (ABDZ24_25140) - 4775541..4775828 (-) 288 WP_000179951.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  ABDZ24_RS25070 (ABDZ24_25145) addA 4775841..4779566 (-) 3726 WP_000572309.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  ABDZ24_RS25075 (ABDZ24_25150) addB 4779563..4783078 (-) 3516 WP_098258258.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  ABDZ24_RS25080 (ABDZ24_25155) lepB 4783195..4783758 (-) 564 WP_048545235.1 signal peptidase I -
  ABDZ24_RS25085 (ABDZ24_25160) - 4783815..4784408 (-) 594 WP_000347517.1 TVP38/TMEM64 family protein -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 142623.83 Da        Isoelectric Point: 4.8652

>NTDB_id=998149 ABDZ24_RS25070 WP_000572309.1 4775841..4779566(-) (addA) [Bacillus paramobilis strain N24509]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIISEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEAEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADAVLLGKLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVQDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPGEIYDYDTSWK
VEVVDGGTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYDEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKMRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITEEVIREQISGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGQSGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVKIEE

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=998149 ABDZ24_RS25070 WP_000572309.1 4775841..4779566(-) (addA) [Bacillus paramobilis strain N24509]
ATGATTGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAGTGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCAGCAGCAGCTGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAGTG
AAGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGAGCCAGGGTCGCAGCACGTAAGAAAGCAGCTTAGCTTATTAAATAAAGC
TTCTATTTCAACGATTCACTCCTTTTGTTTACAAGTAATTAGAGGATACTACTACATGCTTGATGTTGATCCTCGTTTTC
GCATTGCGAATCAAACAGAAAACGAATTATTAAAAGAAGAAGTATTAGACGACATATTAGAAGCAGAGTATGGAATTGAA
GATAATACGATATTCTTTGAACTCGTTGATCGTTATACGAGTGATCGTAGTGATGATGACTTACAGCGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATACGACGTAGAAGGAA
AGACAATTGAAGATTTAGTGTATGCTTCTTACTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAACATATT
CGTAAAGCAACCGAACTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCGTTGAAACCCTCCAAGCTGATGCAGTTTTACT
CGGAAAGTTATCGTCAGCTGCTCGTGAGTCATGGACAAGTGTGTACGAAGCGATGCAAAACGTATCGTGGCAAACATTAA
AGCGTATTAAGAAAAGTGATTACAACGAAGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGTTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAGCTTGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAACGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAATGGTGAGATGAAGCCATCAGCAGTAGCGCTCCAA
TATCGCAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTCGTACAGGAATCCATTATTAAATTTGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTAGGGGACGTAAAACAGTCGATTTATCGTTTCCGACTAGCAG
AACCAGGTTTATTCCTAGGAAAGTATAAACGCTTCACACAAGAAGGATTGGGCGGCGGAATGAAAATTGATTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCAGGTACGAACTTTATTTTTAAACAAATTATGGGTGAAGAAGTCGGAGAAAT
CGACTATGATGCAGACGCTGAATTAAAGTTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCGGCAGAACTACTATGCA
TTCAGCAAACGGAAGAAGAAGTGCAAGACGGTGAAGAAGGTGCAGAAGTAGAAAAAGCGCAGCTTGAAGCTCGTCTTATG
GCGCAGCGCATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGCGACTTCGTTATTTTACTTCGCTCTATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTAATCGATAAT
CCGATGCAAGATATTCCACTTGCCGCAGTGCTTCGTTCACCAATCGTTGGATTAAATGATGAAGAACTTGCGACGCTTCG
TGCTCATGGGAAGAAAGGTTCGTTTTATGAAGTGATGAGCTCATTCTTAAAAGGGGCACCGCTTGAGGAAGAACAAGAAC
TACATGATAAATTAGAGTGGTTCTATAACTTACTGCAAGGATGGCGTGAATTTGCGCGTCAACAGTCTCTTTCTGATTTG
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTTGTTGGCGGTTTACCAGCCGGAAAGCAAAGGCAAGCAAACCT
GCGTGTACTTTATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGATTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGTGGTGACGATATGGGGACGGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGTATTATGACGATT
CATAAAAGTAAAGGATTAGAGTTCCCAGTCGTGTTTGTTGCGGGACTTGGTCGTCGTTTTAATACACAGGACTTAATGAA
ACGCTTCTTACTTCATAAAGACTTCGGTTTCGGTTCGCAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CGCAACTAGCAATTAAGCGTAAAATGAAAATGGAATTAATCGCGGAAGAAATGCGCGTATTATACGTAGCGTTAACACGT
GCAAAAGAGAAGTTAATTTTAATCGGAACGGTTAAGGATGCAAATAAGGAAATGGAAAAATGGCTTGATGCAAGGGAGCA
TAGTGAATGGTTATTACCAGATCATATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGTAGTATTCCGGGTGAAATTTATGACTATGACACGAGCTGGAAA
GTCGAAGTTGTAGACGGTGGCACGTTACTTGCGCCAGAACCGGTTCAAGAAGAGAAACAAGAATTACTAGAAGCACTTCG
TGAGAAAAAAGCTGTTCCCCTGCAAAGTGAACGAAAAGAAGAAGTGTACGATAGATTAATGTGGAAGTACGGATATGATG
AAGCAACATCTCACCGCGCGAAACAATCTGTTACGGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCT
TTTATTAAAAAAATGCGTGCGCCAATTAAAACACGTCCTCGTTTTATGGAGAAAAAAGGATTAACATACGCTGAAAGAGG
TACGGCAGTTCATGCTGTCATGCAACATGTTGATTTGAAGAAGCCGATTACAGAAGAAGTGATTCGGGAGCAAATTAGTG
GCATGGTTAATAAAGAACTGTTAACATTCGAACAAGCCGAAGAAATAGCAATCGAAAAAGTCATTTCATTCTTTGACAGT
GATCTAGGTAAAAGAGTATTAGCGGCGAAAAGCGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGACAGAGTGGGGAATCAATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGACG
GTATCACTTTAATCGACTTTAAAACAGATACGATTGAAGGGAAGTTCCCAGGCGGATTCGATCAAGCGAAACCGATTTTA
GAAGATCGATACAAAGTGCAGCTTTCGTTGTATGCAAAGGCACTTGAAAAAAGCTTGCAACATCCTGTGAAAGAGAAATG
CTTATACTTCTTTGATGGCAATCATGTTGTGAAAATTGAAGAATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1061-1231)

UvrD-like helicase C-terminal domain (UvrD_C)

(476-890)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.087

100

0.533

Multiple sequence alignment