Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KZZ19_RS19520 Genome accession   NZ_CP155559
Coordinates   3797612..3798046 (-) Length   144 a.a.
NCBI ID   WP_000811502.1    Uniprot ID   A0A5C5ACH0
Organism   Bacillus thuringiensis strain HSY204     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3792612..3803046
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZZ19_RS19505 (KZZ19_019500) - 3793633..3794190 (+) 558 WP_237979402.1 PadR family transcriptional regulator -
  KZZ19_RS19510 (KZZ19_019505) metE 3794860..3797148 (+) 2289 WP_237979403.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  KZZ19_RS19515 (KZZ19_019510) comJ 3797186..3797575 (-) 390 WP_088097584.1 competence protein ComJ -
  KZZ19_RS19520 (KZZ19_019515) nucA/comI 3797612..3798046 (-) 435 WP_000811502.1 DNA-entry nuclease Machinery gene
  KZZ19_RS19525 (KZZ19_019520) - 3798284..3799033 (+) 750 WP_088097585.1 DUF3967 domain-containing protein -
  KZZ19_RS19530 (KZZ19_019525) kinB 3799087..3800361 (-) 1275 WP_088097586.1 sporulation sensor histidine kinase KinB -
  KZZ19_RS19535 (KZZ19_019530) - 3800671..3800871 (+) 201 WP_000929285.1 hypothetical protein -
  KZZ19_RS19540 (KZZ19_019535) - 3800995..3802158 (+) 1164 WP_088097587.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16010.38 Da        Isoelectric Point: 8.4331

>NTDB_id=997758 KZZ19_RS19520 WP_000811502.1 3797612..3798046(-) (nucA/comI) [Bacillus thuringiensis strain HSY204]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=997758 KZZ19_RS19520 WP_000811502.1 3797612..3798046(-) (nucA/comI) [Bacillus thuringiensis strain HSY204]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTGTATGAAGTACTTGTTCC
AGAGGAAACAAGTATCAAAAAAACGAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGCGATATCCAGAGACAGGGA
AACATATTACGGATGCTATAAAAGAAGGACATTCAGAAGTTTGTACAATCGATCGTGGTGGCGCTGCGGATAGAAGGAAA
TTATCGTTAGCTCCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCAGCAGATAACCGCGGAGCAGGGTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C5ACH0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

71.171

77.083

0.549

Multiple sequence alignment