Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   KZZ19_RS00530 Genome accession   NZ_CP155559
Coordinates   97683..99059 (+) Length   458 a.a.
NCBI ID   WP_001085204.1    Uniprot ID   A0A0J7D6B4
Organism   Bacillus thuringiensis strain HSY204     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 92683..104059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZZ19_RS00510 (KZZ19_000510) ctsR 92880..93341 (+) 462 WP_001244563.1 transcriptional regulator CtsR -
  KZZ19_RS00515 (KZZ19_000515) - 93511..94059 (+) 549 WP_237982898.1 UvrB/UvrC motif-containing protein -
  KZZ19_RS00520 (KZZ19_000520) - 94064..95128 (+) 1065 WP_098343377.1 protein arginine kinase -
  KZZ19_RS00525 (KZZ19_000525) clpC 95151..97586 (+) 2436 WP_098343376.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  KZZ19_RS00530 (KZZ19_000530) radA 97683..99059 (+) 1377 WP_001085204.1 DNA repair protein RadA Machinery gene
  KZZ19_RS00535 (KZZ19_000535) disA 99063..100136 (+) 1074 WP_000392165.1 DNA integrity scanning diadenylate cyclase DisA -
  KZZ19_RS00540 (KZZ19_000540) - 100297..101400 (+) 1104 WP_088094803.1 PIN/TRAM domain-containing protein -
  KZZ19_RS00545 (KZZ19_000545) ispD 101417..102097 (+) 681 WP_237982314.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  KZZ19_RS00550 (KZZ19_000550) ispF 102212..102688 (+) 477 WP_237982313.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49884.59 Da        Isoelectric Point: 7.4250

>NTDB_id=997730 KZZ19_RS00530 WP_001085204.1 97683..99059(+) (radA) [Bacillus thuringiensis strain HSY204]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQSEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVKGSNLFVVAETDLQRIA
AHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKPTTPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=997730 KZZ19_RS00530 WP_001085204.1 97683..99059(+) (radA) [Bacillus thuringiensis strain HSY204]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGTTATCAGTCACCAAAATATATGGGGAAATGCCCTGGTTG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCGGTTGTATCATCAAGACGCCTTAATTATGCCAATGCAATTCAAT
CGGAAGTAACAAAACCAAGACGCCTAACAGAAGTAGAAACAAAATCCGAGGCACGTATTGAAACGAAATTCCAAGAGTTT
AACCGTGTACTTGGTGGTGGGATTGTAGATGGATCCTTAGTACTGATTGGTGGAGACCCTGGGATTGGGAAATCAACATT
GTTATTACAAATTTCATCGCAATTAGCAGATTCTTCATATGATGTACTATACATATCAGGTGAGGAGTCAGCAAAGCAGA
TTAAACTTCGTGCAGATCGTTTGCATGTAAAGGGTAGTAATCTATTTGTTGTAGCAGAAACTGATCTACAGCGAATTGCA
GCGCACATTGAAGAGATGAATCCAGCTTTTGTTGTTATTGACTCTATTCAAACGATACATTTACCTGAAGTGACGTCAGC
CCCAGGAAGTGTAGCGCAAGTACGTGAATGTACAGCGGAATTAATGAAACTTGCAAAAACGAAAGGAATTCCTATTTTTA
TCGTCGGACATGTGACAAAAGAAGGGGCAATTGCAGGGCCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGATCGTCATCATACATATCGTATCTTGCGAGCTGTGAAGAATCGTTTTGGTTCCACGAATGAAATGGGTATTTT
TGAAATGAAAGAGCTTGGTCTTGCGGAAGTCTTAAATCCTTCTGAAATTTTTCTTGAGGAAAGACCCGTTGGAGTCGCAG
GATCAACAGTAGTTGCCTCAATGGAAGGAACAAGACCGGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAACCCTCGAAGGATGGCGACGGGAATTGATCATAACCGTGTATCGCTTATTATGGCAGTGCTAGAAAAAAGAACAGG
TTTATTATTGCAAAACCAAGACGCATATTTAAAAGTAGCAGGTGGTTTGAAATTAGATGAACCAGCAATTGATTTAGCTG
TGGCTTTAAGTATTGCTTCAAGTTTTAGAGATAAACCTACGACACCAACTGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGTGCTATTATTCC
TAGAAAAAATTTGGGGGGATGGACAATTCCGGATGGGATTGAGGTAGTAGGTGTATCTAATTTAGGGGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(60-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0J7D6B4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.728

100

0.749
  radA Streptococcus pneumoniae Rx1

64.238

98.908

0.635
  radA Streptococcus pneumoniae D39

64.238

98.908

0.635
  radA Streptococcus pneumoniae R6

64.238

98.908

0.635
  radA Streptococcus pneumoniae TIGR4

64.238

98.908

0.635
  radA Streptococcus mitis SK321

64.238

98.908

0.635
  radA Streptococcus mitis NCTC 12261

64.018

98.908

0.633

Multiple sequence alignment