Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ABER85_RS09875 Genome accession   NZ_CP155547
Coordinates   2025781..2026422 (-) Length   213 a.a.
NCBI ID   WP_000201223.1    Uniprot ID   N9LAP0
Organism   Acinetobacter sp. 8_A20231192     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2020781..2031422
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABER85_RS09855 (ABER85_09855) aroK 2021697..2022266 (-) 570 WP_000643712.1 shikimate kinase AroK -
  ABER85_RS09860 (ABER85_09860) pilQ 2022278..2024443 (-) 2166 WP_001017033.1 type IV pilus secretin PilQ family protein Machinery gene
  ABER85_RS09865 (ABER85_09865) pilP 2024506..2025033 (-) 528 WP_000695067.1 pilus assembly protein PilP Machinery gene
  ABER85_RS09870 (ABER85_09870) pilO 2025044..2025784 (-) 741 WP_000076101.1 type 4a pilus biogenesis protein PilO Machinery gene
  ABER85_RS09875 (ABER85_09875) pilN 2025781..2026422 (-) 642 WP_000201223.1 PilN domain-containing protein Machinery gene
  ABER85_RS09880 (ABER85_09880) pilM 2026422..2027480 (-) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  ABER85_RS09885 (ABER85_09885) ponA 2027642..2030197 (+) 2556 WP_253612291.1 penicillin-binding protein PBP1a -
  ABER85_RS09890 (ABER85_09890) - 2030215..2031039 (-) 825 WP_346534475.1 putative RNA methyltransferase -
  ABER85_RS09895 (ABER85_09895) - 2031042..2031374 (-) 333 WP_000169601.1 hypothetical protein -

Sequence

Protein


Download         Length: 213 a.a.        Molecular weight: 23999.36 Da        Isoelectric Point: 5.3968

>NTDB_id=997655 ABER85_RS09875 WP_000201223.1 2025781..2026422(-) (pilN) [Acinetobacter sp. 8_A20231192]
MATINLLPWREELREQRKKQFITLCFGVVVLGITTVFGGWFYFDHKLDDQEQANQLIMSTNQNLDQQLKTLNGLQEQRDA
IIERMKLIQGLQSQRPVVVRLVDELVRVTPPAMYLTKFSRTGDKFTIEGKAESPNTVAELLRNLEASPWYRNAFMNSFLA
NEEKKDKAASSLLPRVEDHYGSFVVTVDLGEMGVATTDDSAKPSTGESVGAAK

Nucleotide


Download         Length: 642 bp        

>NTDB_id=997655 ABER85_RS09875 WP_000201223.1 2025781..2026422(-) (pilN) [Acinetobacter sp. 8_A20231192]
ATGGCAACAATTAATTTACTGCCTTGGCGCGAAGAGCTGAGGGAGCAACGAAAAAAACAATTTATAACGTTATGTTTTGG
GGTGGTTGTTTTGGGGATTACAACCGTATTTGGGGGATGGTTTTATTTTGACCATAAATTAGATGATCAAGAGCAAGCGA
ATCAGCTCATTATGAGCACAAATCAGAACCTTGATCAGCAATTAAAAACATTAAACGGCTTACAGGAACAGCGTGATGCC
ATTATTGAACGCATGAAGTTGATCCAAGGTTTGCAGAGTCAACGTCCAGTAGTGGTAAGACTTGTTGATGAGTTAGTTCG
AGTCACACCACCAGCTATGTATTTAACGAAATTTAGCCGAACCGGAGATAAGTTTACGATTGAAGGTAAAGCTGAGAGTC
CAAATACAGTAGCCGAACTACTCCGTAATCTTGAAGCATCTCCTTGGTATCGTAATGCTTTTATGAACTCTTTCTTGGCT
AATGAAGAGAAAAAAGATAAGGCTGCAAGTTCTCTTCTTCCTAGGGTGGAAGACCACTATGGTAGTTTTGTTGTGACTGT
CGATTTGGGGGAAATGGGCGTGGCCACAACGGATGATTCTGCTAAGCCATCTACAGGTGAAAGTGTGGGGGCTGCTAAAT
GA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-167)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LAP0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Acinetobacter baumannii D1279779

99.061

100

0.991
  comN Acinetobacter baylyi ADP1

72.115

97.653

0.704

Multiple sequence alignment