Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AAH687_RS12200 Genome accession   NZ_CP155530
Coordinates   2388606..2389676 (+) Length   356 a.a.
NCBI ID   WP_024718839.1    Uniprot ID   -
Organism   Bacillus altitudinis strain TN5S8     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2383606..2394676
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAH687_RS12165 (AAH687_12165) - 2384660..2384974 (+) 315 WP_008342314.1 MTH1187 family thiamine-binding protein -
  AAH687_RS12170 (AAH687_12170) - 2385197..2385370 (-) 174 WP_003217333.1 DUF2759 domain-containing protein -
  AAH687_RS12175 (AAH687_12175) - 2385531..2386169 (+) 639 WP_017357989.1 MBL fold metallo-hydrolase -
  AAH687_RS12180 (AAH687_12180) - 2386218..2386460 (-) 243 WP_003217440.1 DUF2626 domain-containing protein -
  AAH687_RS12185 (AAH687_12185) - 2386673..2387053 (+) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  AAH687_RS12195 (AAH687_12195) - 2387279..2388148 (+) 870 WP_346394179.1 STAS domain-containing protein -
  AAH687_RS12200 (AAH687_12200) comGA 2388606..2389676 (+) 1071 WP_024718839.1 competence type IV pilus ATPase ComGA Machinery gene
  AAH687_RS12205 (AAH687_12205) comGB 2389657..2390697 (+) 1041 WP_346394181.1 competence type IV pilus assembly protein ComGB -
  AAH687_RS12210 (AAH687_12210) comGC 2390714..2391008 (+) 295 Protein_2351 competence type IV pilus major pilin ComGC -
  AAH687_RS12215 (AAH687_12215) comGD 2391001..2391447 (+) 447 WP_008342187.1 competence type IV pilus minor pilin ComGD -
  AAH687_RS12220 (AAH687_12220) comGE 2391467..2391745 (+) 279 WP_268438649.1 competence type IV pilus minor pilin ComGE -
  AAH687_RS12225 (AAH687_12225) comGF 2391687..2392184 (+) 498 WP_019743636.1 competence type IV pilus minor pilin ComGF -
  AAH687_RS12230 (AAH687_12230) comGG 2392181..2392564 (+) 384 WP_346394184.1 competence type IV pilus minor pilin ComGG -
  AAH687_RS12235 (AAH687_12235) - 2392625..2392825 (+) 201 WP_060781188.1 YqzE family protein -
  AAH687_RS12240 (AAH687_12240) - 2392860..2393186 (-) 327 WP_056705243.1 DUF3889 domain-containing protein -
  AAH687_RS12245 (AAH687_12245) tapA 2393439..2393966 (+) 528 WP_304717077.1 amyloid fiber anchoring/assembly protein TapA -
  AAH687_RS12250 (AAH687_12250) - 2394016..2394588 (+) 573 WP_017357979.1 signal peptidase I -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40366.72 Da        Isoelectric Point: 9.0449

>NTDB_id=996960 AAH687_RS12200 WP_024718839.1 2388606..2389676(+) (comGA) [Bacillus altitudinis strain TN5S8]
MYGIEYLGQELLEEACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELACPFCGETCELYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEANL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=996960 AAH687_RS12200 WP_024718839.1 2388606..2389676(+) (comGA) [Bacillus altitudinis strain TN5S8]
TTGTATGGTATTGAATATTTGGGGCAAGAGCTGCTAGAAGAGGCGTGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTATCTTCTATGGATATTGGTGAAAGAAGGAGACCACAAAATGGGTCATTGGCT
GTGATGCTAAGACGCGGTCAAGTGTTTGTTCGGCTCTCTACACTACCGACTGTAAATGATGAAAGTTTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCGAAAACAAAGTATTTGTCACTGTTCCCGAAGGCTTCAGCGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACTGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATTCAGTTT
GCCAAAAAACATTTTAATCGAAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCTGGTATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTTG
GAGAAATCAGAGATGCGGAAACAGCCCGAACAGCTATTAGAGCAGCTTTGACAGGTCATTTAGTCATGAGCACATTGCAC
GCAAAGAATGCAAAAGGAGCTCTTTATCGAATGCTCGAATTTGGTGTGACGATGAATGAACTCGAACAAACAATGGTTGC
CATTGCTGCTCAGCGGTTAATCGAACTCGCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCAAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTGCAAAGATTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGCTGGGTTTATGAAGAAGCGAACCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.888

100

0.649

Multiple sequence alignment