Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NSQ96_RS11360 Genome accession   NZ_CP155465
Coordinates   2295051..2296136 (+) Length   361 a.a.
NCBI ID   WP_034771006.1    Uniprot ID   A0A090IWD5
Organism   Caldifermentibacillus hisashii strain FSL K6-2851     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IS/Tn 2293472..2294785 2295051..2296136 flank 266


Gene organization within MGE regions


Location: 2293472..2296136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ96_RS11355 (NSQ96_11355) - 2293472..2294785 (-) 1314 WP_108897317.1 IS1380-like element ISBco1 family transposase -
  NSQ96_RS11360 (NSQ96_11360) comGA 2295051..2296136 (+) 1086 WP_034771006.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 40615.22 Da        Isoelectric Point: 7.5768

>NTDB_id=996536 NSQ96_RS11360 WP_034771006.1 2295051..2296136(+) (comGA) [Caldifermentibacillus hisashii strain FSL K6-2851]
MPNSIETRAREILEHALSLHATDIHIIPKSKHASVLFRLSHKLIPIMTIELEETERLISYMKFQAAMDIGEKRKPQNGSF
QIDIGGLSVGLRLSTLPSIFAESLVIRILPQESYIPFQQLSLFPKSLRLLLAMLKFSHGLIIFTGPTGSGKTTTLYSLLQ
HSTKSLGRNVITLEDPVEKNSEDLLQVQVNEKAGITYNTGLKAILRHDPDIIMVGEIRDSETAHIAIRAALTGHLVLTTM
HTKDSKGALYRLIEFGVNWHEIEQTLVAVTAQRLVELICPYCLEEECPVYCNQNKNKRTAVYEILYGRALKEALLEMKGE
AFSARYPTLGQLIAKGIALGFIKKSEYERWVHDIETKSLEN

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=996536 NSQ96_RS11360 WP_034771006.1 2295051..2296136(+) (comGA) [Caldifermentibacillus hisashii strain FSL K6-2851]
TTGCCGAACTCGATTGAAACACGGGCAAGGGAAATTTTGGAGCACGCCCTTTCTTTACATGCAACAGATATTCATATCAT
CCCCAAATCAAAACATGCTTCCGTTCTATTTCGATTGTCTCATAAACTCATCCCCATAATGACAATCGAACTTGAGGAAA
CGGAAAGATTAATTTCATATATGAAGTTTCAGGCAGCAATGGATATCGGCGAAAAAAGAAAACCCCAGAACGGTTCTTTT
CAAATAGATATCGGGGGGCTATCTGTCGGACTTCGATTATCCACTTTGCCTTCCATATTCGCTGAAAGTTTGGTCATCCG
TATATTACCACAAGAATCATACATCCCATTTCAACAATTATCCTTATTCCCAAAATCACTGAGACTACTGTTAGCCATGT
TAAAGTTTTCCCACGGTTTAATCATATTTACAGGACCAACCGGATCGGGCAAAACAACAACTTTATATTCTCTTCTCCAA
CATTCGACAAAATCTCTTGGCAGGAATGTCATCACCTTGGAAGATCCTGTTGAAAAAAATAGTGAAGATTTACTTCAAGT
TCAAGTAAATGAAAAAGCCGGCATTACGTATAATACCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATTATTATGG
TCGGCGAAATTCGTGATAGTGAGACAGCACATATTGCCATTCGAGCAGCATTAACCGGTCATTTAGTATTGACAACGATG
CATACGAAAGATTCAAAAGGTGCATTGTACCGGTTAATCGAATTTGGTGTTAATTGGCATGAAATTGAACAAACTTTGGT
GGCAGTGACGGCCCAAAGATTAGTCGAATTAATTTGTCCTTACTGTTTAGAAGAAGAATGTCCCGTCTATTGCAACCAGA
ATAAAAATAAGCGTACTGCCGTTTATGAAATTTTGTACGGTCGTGCATTAAAGGAAGCACTGTTGGAAATGAAGGGTGAA
GCATTCTCAGCGCGCTATCCGACTTTGGGCCAATTGATTGCAAAAGGTATTGCTCTCGGATTTATCAAGAAAAGTGAGTA
TGAAAGATGGGTGCATGATATTGAGACAAAGTCGCTGGAAAATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A090IWD5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.695

97.23

0.551

Multiple sequence alignment