Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   EW037_RS20365 Genome accession   NZ_LR130562
Coordinates   4215006..4216388 (-) Length   460 a.a.
NCBI ID   WP_001029698.1    Uniprot ID   A0A2T3RLH6
Organism   Escherichia coli strain MS14384 isolate MS14384     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4210006..4221388
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW037_RS20345 ettA 4210276..4211943 (+) 1668 WP_000046749.1 energy-dependent translational throttle protein EttA -
  EW037_RS20350 - 4212023..4213371 (+) 1349 WP_101687415.1 IS3-like element IS1397 family transposase -
  EW037_RS20355 nadS 4213435..4213719 (-) 285 WP_000007436.1 NadS family protein -
  EW037_RS20360 nadR 4213753..4214985 (-) 1233 WP_000093813.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  EW037_RS20365 radA/sms 4215006..4216388 (-) 1383 WP_001029698.1 DNA repair protein RadA Machinery gene
  EW037_RS20370 serB 4216437..4217405 (-) 969 WP_001132956.1 phosphoserine phosphatase -
  EW037_RS20375 ytjB 4217511..4218155 (+) 645 WP_000124615.1 YtjB family periplasmic protein -
  EW037_RS20380 lplA 4218183..4219199 (+) 1017 WP_063815170.1 lipoate--protein ligase LplA -
  EW037_RS20385 yjjJ 4219200..4220531 (-) 1332 WP_063815169.1 type II toxin-antitoxin system HipA family toxin YjjJ -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49442.02 Da        Isoelectric Point: 7.1936

>NTDB_id=996319 EW037_RS20365 WP_001029698.1 4215006..4216388(-) (radA/sms) [Escherichia coli strain MS14384 isolate MS14384]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=996319 EW037_RS20365 WP_001029698.1 4215006..4216388(-) (radA/sms) [Escherichia coli strain MS14384 isolate MS14384]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGCGGCT
ATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAGGAGTTGCCGCGTTTTTCAACCGGA
TTTAAAGAGTTCGATCGCGTATTAGGCGGTGGCGTAGTGCCAGGAAGCGCCATTCTGATTGGCGGTAACCCAGGCGCGGG
GAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGCGCAGCAGATGAAAACGCTATATGTCACTGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAG
CAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCCATCCAGGTGATGCATATGGCGGATGT
ACAATCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGCGTGG
CGATTGTCATGGTTGGGCACGTAACCAAAGATGGCTCGCTGGCTGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCG
GTGCTTCTTGATGGCGATGCCGACTCCCGTTTTCGCACCTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCT
GGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTTAGCAACCCTTCCGCCATCTTTTTAAGCCGCGGTGATGAAG
TGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCATTGGTCGATCAC
TCGATGATGGCGAATCCACGCCGCGTGGCAGTGGGGCTGGAGCAAAACCGTCTGGCAATCCTGCTGGCGGTGCTGCACCG
TCACGGTGGGTTGCAAATGGCCGATCAGGATGTATTTGTTAACGTGGTCGGCGGCGTGAAGGTGACGGAAACCAGCGCCG
ACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGTATCTCAGAAGCGGCGAAACATGGTTTTCGGCGGGC
GATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3RLH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.827

99.348

0.465
  radA Streptococcus mitis SK321

43.86

99.13

0.435
  radA Streptococcus pneumoniae D39

43.421

99.13

0.43
  radA Streptococcus pneumoniae TIGR4

43.421

99.13

0.43
  radA Streptococcus mitis NCTC 12261

43.421

99.13

0.43
  radA Streptococcus pneumoniae R6

43.421

99.13

0.43
  radA Streptococcus pneumoniae Rx1

43.421

99.13

0.43