Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AAFX13_RS23705 Genome accession   NZ_CP155084
Coordinates   3254864..3255880 (-) Length   338 a.a.
NCBI ID   WP_031815696.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain TBV028     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3249864..3260880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFX13_RS23680 (AAFX13_23685) aroK 3250724..3251242 (-) 519 WP_005381319.1 shikimate kinase AroK -
  AAFX13_RS23685 (AAFX13_23690) pilQ 3251431..3253179 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  AAFX13_RS23690 (AAFX13_23695) pilP 3253211..3253726 (-) 516 WP_025554074.1 pilus assembly protein PilP Machinery gene
  AAFX13_RS23695 (AAFX13_23700) pilO 3253716..3254309 (-) 594 WP_029863582.1 type 4a pilus biogenesis protein PilO Machinery gene
  AAFX13_RS23700 (AAFX13_23705) pilN 3254302..3254880 (-) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  AAFX13_RS23705 (AAFX13_23710) pilM 3254864..3255880 (-) 1017 WP_031815696.1 type IV pilus biogenesis protein PilM Machinery gene
  AAFX13_RS23710 (AAFX13_23715) - 3256023..3258593 (+) 2571 WP_031815695.1 penicillin-binding protein 1A -
  AAFX13_RS23715 (AAFX13_23720) oxyR 3258649..3259557 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  AAFX13_RS23720 (AAFX13_23725) - 3259728..3260456 (+) 729 WP_031815694.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37834.31 Da        Isoelectric Point: 6.6639

>NTDB_id=996224 AAFX13_RS23705 WP_031815696.1 3254864..3255880(-) (pilM) [Vibrio parahaemolyticus strain TBV028]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHREFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGERALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=996224 AAFX13_RS23705 WP_031815696.1 3254864..3255880(-) (pilM) [Vibrio parahaemolyticus strain TBV028]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCTGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGCTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAGGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAATGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCTGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGAGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.615

100

0.846
  pilM Vibrio cholerae strain A1552

47.941

100

0.482

Multiple sequence alignment