Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   EJ099_RS24030 Genome accession   NZ_LR130536
Coordinates   5097779..5098246 (-) Length   155 a.a.
NCBI ID   WP_003161762.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa isolate paerg010     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5092779..5103246
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJ099_RS24015 (BANRA_04668) nadC 5095279..5096127 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EJ099_RS24025 (BANRA_04670) - 5096308..5097705 (-) 1398 WP_014603302.1 O-antigen ligase family protein -
  EJ099_RS24030 (BANRA_04671) pilA/pilAI 5097779..5098246 (-) 468 WP_003161762.1 pilin Machinery gene
  EJ099_RS24035 (BANRA_04672) pilB 5098477..5100177 (+) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EJ099_RS24040 (BANRA_04673) pilC 5100181..5101398 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  EJ099_RS24045 (BANRA_04674) pilD 5101399..5102271 (+) 873 WP_003107301.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  EJ099_RS24050 (BANRA_04675) coaE 5102268..5102879 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  EJ099_RS24055 (BANRA_04676) yacG 5102876..5103076 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 15959.30 Da        Isoelectric Point: 9.0145

>NTDB_id=995864 EJ099_RS24030 WP_003161762.1 5097779..5098246(-) (pilA/pilAI) [Pseudomonas aeruginosa isolate paerg010]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSKDNPADGEYDLGFTK
STLLAGNDGKAQITITGESSATPTIAGTLGNSAGKAISGAVITIKRSAEGVWTCAISGSPANWKANYAPANCPKS

Nucleotide


Download         Length: 468 bp        

>NTDB_id=995864 EJ099_RS24030 WP_003161762.1 5097779..5098246(-) (pilA/pilAI) [Pseudomonas aeruginosa isolate paerg010]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGTTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTTGTTTCCAAGGATAATCCCGCGGATGGGGAATATGATCTTGGTTTTACCAAG
TCTACTTTGCTTGCTGGCAACGACGGTAAGGCACAGATCACCATCACTGGCGAAAGCAGTGCAACCCCGACCATTGCGGG
GACTCTGGGTAACTCTGCTGGTAAGGCCATCAGCGGTGCCGTTATCACCATCAAGCGTAGTGCTGAGGGAGTCTGGACCT
GCGCTATCAGTGGGTCTCCGGCCAACTGGAAAGCCAACTACGCTCCGGCCAACTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.679

100

0.477
  pilA Pseudomonas aeruginosa PAK

40.964

100

0.439
  pilA Acinetobacter baumannii strain A118

43.312

100

0.439
  pilA Ralstonia pseudosolanacearum GMI1000

37.427

100

0.413
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.262

90.968

0.394
  pilA Vibrio cholerae strain A1552

43.262

90.968

0.394
  pilA Vibrio cholerae C6706

43.262

90.968

0.394
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.333

96.774

0.381
  pilA/pilA1 Eikenella corrodens VA1

38.095

94.839

0.361

Multiple sequence alignment