Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   AAVB74_RS02850 Genome accession   NZ_CP154920
Coordinates   532419..533564 (-) Length   381 a.a.
NCBI ID   WP_017696253.1    Uniprot ID   A0A0C3JF17
Organism   Bacillus subtilis strain FUA2232     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 527419..538564
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAVB74_RS02825 (AAVB74_02825) pbpG 527683..529758 (-) 2076 WP_345805801.1 transglycosylase domain-containing protein -
  AAVB74_RS02830 (AAVB74_02830) speE 529960..530790 (+) 831 WP_003227543.1 spermidine synthase -
  AAVB74_RS02835 (AAVB74_02835) speB 530851..531723 (+) 873 WP_003227545.1 agmatinase -
  AAVB74_RS02840 (AAVB74_02840) ywhH 531755..532217 (-) 463 Protein_531 YbaK/EbsC family protein -
  AAVB74_RS02845 (AAVB74_02845) - 532316..532435 (-) 120 WP_014665774.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  AAVB74_RS02850 (AAVB74_02850) rapF 532419..533564 (-) 1146 WP_017696253.1 tetratricopeptide repeat protein Regulator
  AAVB74_RS02855 (AAVB74_02855) - 533567..533797 (+) 231 WP_041334639.1 hypothetical protein -
  AAVB74_RS02860 (AAVB74_02860) ywhK 533794..535149 (+) 1356 WP_015250986.1 YncE family protein -
  AAVB74_RS02865 (AAVB74_02865) ywhL 535188..536564 (+) 1377 WP_345805802.1 YncE family protein -
  AAVB74_RS02870 (AAVB74_02870) albG 536570..537271 (-) 702 WP_345805803.1 antilisterial bacteriocin subtilosin biosynthesis protein AlbG -
  AAVB74_RS02875 (AAVB74_02875) albF 537268..538548 (-) 1281 WP_003227558.1 pitrilysin family protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45593.24 Da        Isoelectric Point: 5.0684

>NTDB_id=995428 AAVB74_RS02850 WP_017696253.1 532419..533564(-) (rapF) [Bacillus subtilis strain FUA2232]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDRREYVLAIQFFKKAESKLVFVKDKIDKAEFFFKMSEVYYYMKQTYVS
MDYARQAFEIYSEQTTYDIRMIQCQSLFGANFLDLKQYEKAIAHFQKAFTLAKTEQQPQLMGRTLYNIALCKQNQNSFES
AIEYVKKSIQVFEGSKMLPSLPQAFFLITQIYFKLGKIDNACEYHKKGKEYSAAAGDTVYLTEFEFLESLYLSGPDEERI
EKCFVFLESKMMYADIEDFALDVAKYYHQQENYKKASHYFLKVEEARQHIQGGVNLYEMEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=995428 AAVB74_RS02850 WP_017696253.1 532419..533564(-) (rapF) [Bacillus subtilis strain FUA2232]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTAATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAATTTCTTCAG
AGGCATGTATGAACTGGATCGCCGTGAATATGTATTAGCCATTCAATTCTTTAAAAAAGCTGAGAGCAAGCTGGTTTTTG
TCAAAGATAAAATCGATAAAGCTGAATTCTTTTTCAAAATGTCAGAAGTCTATTATTATATGAAACAAACTTATGTCTCC
ATGGACTATGCACGTCAAGCATTCGAAATATACAGTGAACAGACTACATATGATATCAGAATGATTCAATGCCAGTCCTT
ATTCGGAGCCAACTTTTTGGATTTAAAACAATACGAAAAAGCAATTGCCCATTTTCAAAAAGCCTTTACACTTGCAAAAA
CAGAGCAGCAGCCCCAATTAATGGGAAGAACACTTTATAACATAGCACTCTGCAAGCAAAACCAAAACAGTTTTGAATCA
GCGATAGAGTATGTAAAAAAATCTATTCAAGTATTTGAAGGATCTAAAATGCTTCCTTCATTGCCACAAGCATTTTTCCT
AATTACACAAATTTATTTTAAATTAGGTAAGATTGATAACGCCTGTGAATATCATAAAAAAGGAAAAGAGTATTCTGCAG
CTGCGGGAGATACCGTGTATCTAACGGAGTTTGAATTTTTGGAATCTTTATACTTGTCTGGACCGGATGAGGAACGGATA
GAAAAATGTTTTGTTTTTCTTGAAAGTAAAATGATGTATGCAGATATTGAGGACTTCGCACTTGATGTTGCTAAATATTA
TCATCAACAGGAAAATTACAAAAAAGCATCTCATTACTTTTTAAAGGTTGAAGAAGCAAGACAGCACATTCAAGGGGGGG
TGAATTTATATGAAATGGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(4-65)

Tetratricopeptide repeat (TPR_8)

(263-290)

Tetratricopeptide repeat (TPR_12)

(189-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C3JF17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

80.052

100

0.801
  rapC Bacillus subtilis subsp. subtilis str. 168

57.105

99.738

0.57

Multiple sequence alignment