Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AAYR29_RS03155 Genome accession   NZ_CP154918
Coordinates   544269..544679 (+) Length   136 a.a.
NCBI ID   WP_160221688.1    Uniprot ID   -
Organism   Bacillus subtilis strain FUA2231     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 539269..549679
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAYR29_RS03120 (AAYR29_03120) arsR 539432..539749 (+) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -
  AAYR29_RS03125 (AAYR29_03125) yqcK 539810..540250 (+) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  AAYR29_RS03130 (AAYR29_03130) acr3 540273..541313 (+) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  AAYR29_RS03135 (AAYR29_03135) arsC 541325..541744 (+) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  AAYR29_RS03140 (AAYR29_03140) - 542099..542277 (+) 179 Protein_568 hypothetical protein -
  AAYR29_RS03145 (AAYR29_03145) spoIVCA 542235..543695 (+) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  AAYR29_RS03150 (AAYR29_03150) - 543726..544073 (-) 348 Protein_570 sigma-70 family RNA polymerase sigma factor -
  AAYR29_RS03155 (AAYR29_03155) nucA/comI 544269..544679 (+) 411 WP_160221688.1 sporulation-specific Dnase NucB Machinery gene
  AAYR29_RS03160 (AAYR29_03160) yqeB 544712..545434 (-) 723 WP_345806059.1 DUF308 domain-containing protein -
  AAYR29_RS03165 (AAYR29_03165) gnd 545686..546579 (+) 894 WP_345806060.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  AAYR29_RS03170 (AAYR29_03170) yqeD 546598..547224 (-) 627 WP_038427798.1 TVP38/TMEM64 family protein -
  AAYR29_RS03175 (AAYR29_03175) cwlH 547411..548163 (+) 753 WP_087961324.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  AAYR29_RS03180 (AAYR29_03180) yqeF 548415..549146 (+) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  AAYR29_RS03185 (AAYR29_03185) - 549452..549592 (-) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14994.06 Da        Isoelectric Point: 5.1853

>NTDB_id=995349 AAYR29_RS03155 WP_160221688.1 544269..544679(+) (nucA/comI) [Bacillus subtilis strain FUA2231]
MKKWMAGLFLAAAVLLCLMVPQQIQGASLYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=995349 AAYR29_RS03155 WP_160221688.1 544269..544679(+) (nucA/comI) [Bacillus subtilis strain FUA2231]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTTGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment