Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   AAVC46_RS07130 Genome accession   NZ_CP154913
Coordinates   1362558..1363328 (-) Length   256 a.a.
NCBI ID   WP_012010612.1    Uniprot ID   A8FFB2
Organism   Bacillus pumilus strain FUA2119     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 1357558..1368328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAVC46_RS07110 (AAVC46_07110) - 1357648..1358469 (-) 822 WP_008342399.1 pyruvate, water dikinase regulatory protein -
  AAVC46_RS07115 (AAVC46_07115) - 1358495..1359133 (-) 639 WP_024423017.1 helix-turn-helix transcriptional regulator -
  AAVC46_RS07120 (AAVC46_07120) glyS 1359300..1361345 (-) 2046 WP_340973340.1 glycine--tRNA ligase subunit beta -
  AAVC46_RS07125 (AAVC46_07125) glyQ 1361338..1362225 (-) 888 WP_012010611.1 glycine--tRNA ligase subunit alpha -
  AAVC46_RS07130 (AAVC46_07130) recO 1362558..1363328 (-) 771 WP_012010612.1 DNA repair protein RecO Machinery gene
  AAVC46_RS07135 (AAVC46_07135) - 1363364..1363507 (-) 144 WP_003217288.1 YqzL family protein -
  AAVC46_RS07140 (AAVC46_07140) era 1363656..1364561 (-) 906 WP_099726610.1 GTPase Era -
  AAVC46_RS07145 (AAVC46_07145) - 1364554..1364952 (-) 399 WP_034661347.1 cytidine deaminase -
  AAVC46_RS07150 (AAVC46_07150) - 1365066..1365452 (-) 387 WP_334749775.1 diacylglycerol kinase family protein -
  AAVC46_RS07155 (AAVC46_07155) ybeY 1365430..1365909 (-) 480 WP_012010615.1 rRNA maturation RNase YbeY -
  AAVC46_RS07160 (AAVC46_07160) - 1365910..1367997 (-) 2088 WP_334750286.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29230.90 Da        Isoelectric Point: 8.4348

>NTDB_id=995309 AAVC46_RS07130 WP_012010612.1 1362558..1363328(-) (recO) [Bacillus pumilus strain FUA2119]
MLIKSEGIVLRTTDYGETNKIVTLLTREHGKIGVMARGAKKSNSRLSAISQPFLYGTFLIQSSTGLGTLQQGEMIESMRT
IREDLFLTAYAAYMTELLDKGTEEKKPNPYLFELLLQSLRHLNEGTDADIILFIVEVKMLSVMGMKPELDQCVHCGQKEG
QFHFSIRDNGFICQNCFSKDPYKLPLSPAAARLLRLFHYFDLSRLGQVDVKPQTKQEIRQVLDHYYDEYSGLYLKSKKFM
NQMESMKKLMGGENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=995309 AAVC46_RS07130 WP_012010612.1 1362558..1363328(-) (recO) [Bacillus pumilus strain FUA2119]
ATGCTCATTAAAAGTGAAGGAATCGTCCTTAGAACAACAGATTATGGCGAAACAAACAAAATTGTCACGCTGCTCACTAG
AGAACATGGGAAAATTGGCGTGATGGCAAGAGGTGCAAAAAAATCAAACAGCCGCCTGTCAGCCATAAGCCAGCCGTTTT
TATACGGAACCTTCCTGATCCAATCATCAACAGGACTTGGAACGCTCCAGCAAGGCGAAATGATTGAAAGTATGCGAACC
ATTCGGGAGGATTTATTTTTGACCGCATATGCCGCATATATGACAGAGTTACTCGATAAAGGCACAGAAGAAAAAAAGCC
AAACCCTTATTTGTTTGAGCTCCTGCTTCAATCTCTGCGTCATCTGAATGAAGGCACAGATGCTGATATTATCTTATTTA
TCGTAGAGGTCAAAATGTTATCAGTAATGGGAATGAAGCCTGAACTTGATCAATGTGTCCATTGTGGTCAAAAAGAAGGG
CAGTTTCATTTCTCTATTAGAGATAACGGCTTCATTTGCCAAAATTGCTTTTCTAAAGATCCGTATAAACTGCCCTTATC
CCCAGCAGCTGCAAGGCTGCTCCGTTTATTTCATTATTTTGATTTATCAAGGCTCGGTCAGGTTGATGTGAAACCGCAGA
CGAAACAAGAAATTCGTCAAGTGCTCGATCACTACTATGACGAGTATTCAGGCCTTTATTTGAAATCAAAAAAATTCATG
AATCAGATGGAATCCATGAAAAAGCTAATGGGCGGGGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A8FFB2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

75.391

100

0.754

Multiple sequence alignment