Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AAYR27_RS02340 Genome accession   NZ_CP154912
Coordinates   484503..484931 (+) Length   142 a.a.
NCBI ID   WP_024424305.1    Uniprot ID   A0A5C0WGQ8
Organism   Bacillus safensis strain FUA2118     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 479503..489931
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAYR27_RS02330 (AAYR27_02330) - 480088..482076 (-) 1989 WP_305933818.1 hydantoinase B/oxoprolinase family protein -
  AAYR27_RS02335 (AAYR27_02335) - 482066..484135 (-) 2070 WP_176967334.1 hydantoinase/oxoprolinase family protein -
  AAYR27_RS02340 (AAYR27_02340) nucA/comI 484503..484931 (+) 429 WP_024424305.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  AAYR27_RS02345 (AAYR27_02345) - 485059..486756 (+) 1698 WP_046313811.1 methyl-accepting chemotaxis protein -
  AAYR27_RS02350 (AAYR27_02350) - 486877..487263 (+) 387 WP_326133455.1 competence protein ComJ -
  AAYR27_RS02355 (AAYR27_02355) - 487265..488071 (-) 807 WP_101677032.1 AAC(3) family N-acetyltransferase -
  AAYR27_RS02360 (AAYR27_02360) - 488100..488516 (-) 417 WP_180951418.1 Lrp/AsnC family transcriptional regulator -
  AAYR27_RS02365 (AAYR27_02365) - 488632..489396 (+) 765 WP_326133453.1 MBL fold metallo-hydrolase -
  AAYR27_RS02370 (AAYR27_02370) - 489440..489769 (-) 330 WP_024424311.1 YckD family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15971.96 Da        Isoelectric Point: 6.2215

>NTDB_id=995221 AAYR27_RS02340 WP_024424305.1 484503..484931(+) (nucA/comI) [Bacillus safensis strain FUA2118]
MKLLKMILLLLLIVIGVATGYIQLEQGKQETANSSYDKTIHFPSDRYPETAKHIEEAIDEGHSSICTIDRKHSDEQRDRS
LHGVPTKRGYDRDEWPMAMCKEGGTGASVKYVRPSDNRGAGSWVSHQLSDDPDGTRIQFIID

Nucleotide


Download         Length: 429 bp        

>NTDB_id=995221 AAYR27_RS02340 WP_024424305.1 484503..484931(+) (nucA/comI) [Bacillus safensis strain FUA2118]
ATGAAACTTCTTAAAATGATTCTCTTGCTTTTATTGATTGTTATTGGTGTAGCAACAGGCTATATCCAGCTGGAGCAAGG
GAAACAAGAGACAGCGAATTCATCTTATGACAAAACGATTCATTTTCCATCAGACCGCTATCCCGAAACAGCCAAGCACA
TAGAAGAAGCCATTGATGAAGGTCATTCGTCCATTTGTACAATTGACCGTAAACATAGTGATGAGCAGAGGGATCGATCA
TTACATGGCGTTCCAACAAAGCGCGGGTATGATCGTGACGAATGGCCAATGGCGATGTGCAAAGAAGGAGGCACAGGAGC
TTCTGTCAAATATGTACGCCCTTCTGATAATAGAGGCGCAGGCTCGTGGGTAAGTCATCAATTATCGGATGATCCAGACG
GCACAAGAATCCAATTTATCATCGATTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WGQ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

66.667

80.282

0.535

Multiple sequence alignment