Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AAVC94_RS08695 Genome accession   NZ_CP154906
Coordinates   1732574..1732954 (+) Length   126 a.a.
NCBI ID   WP_223251181.1    Uniprot ID   -
Organism   Bacillus pumilus strain FUA2115     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1727574..1737954
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAVC94_RS08685 (AAVC94_08685) - 1728063..1731206 (+) 3144 WP_253622575.1 bifunctional cytochrome P450/NADPH--P450 reductase -
  AAVC94_RS08690 (AAVC94_08690) - 1731403..1732311 (+) 909 WP_003212025.1 trypsin-like serine protease -
  AAVC94_RS08695 (AAVC94_08695) nucA/comI 1732574..1732954 (+) 381 WP_223251181.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  AAVC94_RS08700 (AAVC94_08700) - 1733103..1733951 (+) 849 WP_050945459.1 STAS domain-containing protein -
  AAVC94_RS08705 (AAVC94_08705) - 1733984..1734526 (-) 543 WP_003211360.1 IseA DL-endopeptidase inhibitor family protein -
  AAVC94_RS08710 (AAVC94_08710) - 1734828..1735262 (+) 435 WP_003212431.1 hypothetical protein -
  AAVC94_RS08715 (AAVC94_08715) lexA 1735309..1735929 (-) 621 WP_003212311.1 transcriptional repressor LexA -
  AAVC94_RS08720 (AAVC94_08720) yneA 1736089..1736400 (+) 312 WP_003211955.1 cell division suppressor protein YneA -
  AAVC94_RS08725 (AAVC94_08725) - 1736418..1737065 (+) 648 WP_060596421.1 recombinase family protein -
  AAVC94_RS08730 (AAVC94_08730) - 1737135..1737365 (+) 231 WP_003211509.1 DUF896 domain-containing protein -

Sequence

Protein


Download         Length: 126 a.a.        Molecular weight: 13785.42 Da        Isoelectric Point: 7.3831

>NTDB_id=995066 AAVC94_RS08695 WP_223251181.1 1732574..1732954(+) (nucA/comI) [Bacillus pumilus strain FUA2115]
MGTLLGGFGEEHAAKGADRYDHVIQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQMSGYPDGKRILFIVK

Nucleotide


Download         Length: 381 bp        

>NTDB_id=995066 AAVC94_RS08695 WP_223251181.1 1732574..1732954(+) (nucA/comI) [Bacillus pumilus strain FUA2115]
ATGGGCACGCTGCTAGGTGGATTTGGGGAGGAACATGCGGCAAAAGGTGCGGATCGATATGATCATGTTATTCAATTTCC
AAAGGAACGGTACCCTGAAACAGGCAGTCATATTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACGATAGACC
GAAATGGAGCAGATGCCCGCAGGCAAGAATCATTAAAAGGAATTCCGACTAAGCCTGGATTCGACCGAGATGAGTGGCCG
ATGGCAGTTTGTCTTGAAGGAGGAAAAGGGGCGAGTGTTCAATATGTCAGTCCGTCTGATAATAGGGGAGCGGGCTCATG
GGTCGGGCACCAGATGAGCGGGTATCCTGATGGGAAAAGAATTTTATTTATTGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(29-115)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.983

94.444

0.548

Multiple sequence alignment