Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   EW031_RS05345 Genome accession   NZ_LR130518
Coordinates   1020991..1022799 (+) Length   602 a.a.
NCBI ID   WP_000082731.1    Uniprot ID   A0A0H2XIW1
Organism   Staphylococcus aureus strain BPH2986 isolate BPH2986     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1015991..1027799
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW031_RS05315 opp4C 1016145..1017026 (+) 882 WP_001180262.1 oligopeptide ABC transporter permease -
  EW031_RS05320 trpS 1017068..1018057 (-) 990 WP_000448934.1 tryptophan--tRNA ligase -
  EW031_RS05325 spxA 1018352..1018747 (+) 396 WP_000258003.1 transcriptional regulator SpxA -
  EW031_RS05330 mecA 1019118..1019837 (+) 720 WP_001217730.1 adaptor protein MecA -
  EW031_RS05340 - 1019958..1020943 (+) 986 Protein_1000 competence protein CoiA -
  EW031_RS05345 pepF 1020991..1022799 (+) 1809 WP_000082731.1 oligoendopeptidase F Regulator
  EW031_RS05350 yjbH 1023259..1024065 (-) 807 WP_000896697.1 protease adaptor protein YjbH -
  EW031_RS05355 - 1024088..1024453 (-) 366 WP_000214075.1 truncated hemoglobin YjbI -
  EW031_RS05360 - 1024557..1025150 (-) 594 WP_000224622.1 CYTH domain-containing protein -
  EW031_RS05365 - 1025336..1025683 (+) 348 WP_001242102.1 hypothetical protein -
  EW031_RS05370 - 1025700..1026335 (+) 636 WP_001077683.1 GTP pyrophosphokinase -
  EW031_RS05375 - 1026352..1027161 (+) 810 WP_001270834.1 NAD kinase -

Sequence

Protein


Download         Length: 602 a.a.        Molecular weight: 69819.39 Da        Isoelectric Point: 4.9226

>NTDB_id=994942 EW031_RS05345 WP_000082731.1 1020991..1022799(+) (pepF) [Staphylococcus aureus strain BPH2986 isolate BPH2986]
MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGKEEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYA
HLKQDQDTTNDKYTGMESRAHQLIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEK
LLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDGNAHPLTQGTFIKYLETDDRKLRESAFRNVYKAYGAHNNTLGATLAG
EVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLVKTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMP
YEEAKEWMLKALEPMGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVHEFGHSAHS
YFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQELERFRATLFRQTMFAEFEHKIHAIEEAGEP
LTPTRMNEEYAKLNKLYFGDSVETDEDISKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKG
SSNYPIEILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA

Nucleotide


Download         Length: 1809 bp        

>NTDB_id=994942 EW031_RS05345 WP_000082731.1 1020991..1022799(+) (pepF) [Staphylococcus aureus strain BPH2986 isolate BPH2986]
ATGAGTCAACAATTATCGAGAGAAGAACAGGAACGTAAATATCCTGAATATACATGGGACTTAACAACAATTTTCAAAGA
TGATGAAGCTTTTGAGGCTGCATTTAAAGAAGTTGAAAATGAGTTAGGCAAAGAAGAACAATTTAAAGGACACATTGGTG
ATAGTGCTGAGACATTATACAATGCGTTAGAATTAGAAGATACATTAGGTACTAAATTAGAAAAAGTATATGTATACGCG
CACCTAAAACAAGACCAAGATACAACGAACGACAAGTATACTGGTATGGAGTCAAGAGCACATCAATTAATTATTAAATT
TAGCTCGGCATGGAGTTTCTTAGTGCCAGAGATTTTACAAATTGATGAAGATAAAATTCAATCATTTGTAAATTCATATG
ATAAATTACAAAAATTCGCATTTGATTTGAAGTTGATTAATGAAAAACGTCCTCATATTTTAGATGCTGAAACTGAAAAG
TTATTAACAGAAGCGCAGGACGCGTTATCAACGCCATCAAATGTATACGGTATGTTTAGCAACGCTGATTTAGTATTTGA
AGATGCGATAGATAAAGATGGAAATGCACACCCGTTAACACAAGGTACATTTATTAAGTATTTAGAAACAGATGATCGCA
AACTAAGAGAAAGTGCTTTTAGAAATGTATATAAAGCATATGGTGCTCATAATAATACGCTTGGCGCTACGCTAGCAGGT
GAAGTGAAGAAAAATGTATTTAATGCTCGTACACACAATTACAAAACTGCAAGAGAAAAAGCATTGAGTAATAATCATAT
TCCAGAAAATGTATATGACAATCTAGTAAAAACTGTACATAAATATTTACCATTGCTACATAGATATACTGAATTGCGCA
AAGAATTGCTAGGTTTAGATGACTTGAAAATGTATGATTTATATACACCATTAATTAAAGATATTAAGTTTGAAATGCCT
TATGAAGAAGCTAAAGAGTGGATGTTAAAAGCATTAGAACCAATGGGTGAAGAATATTTAAATGTAGTTAAAGAAGGCTT
AAACAATCGTTGGGTCGATGTCTATGAGAATAAAGGTAAACGTTCAGGTGGCTATTCATCAGGTGCACATTTAACTAATC
CATTTATTCTACTTAACTGGTCTAATACTATTTCAGACTTATACACATTAGTTCATGAATTTGGGCATTCAGCACATAGT
TACTTCAGTAGAAAATTCCAACCGTCAAATTCTAGTGACTACACTATTTTTGTCGCTGAAGTTGCATCAACTTGTAACGA
AGCACTTTTAAGTGATTATATGGATAAACATCTTGATGATGAAAAACGCTTATTATTATTAAACCAAGAATTAGAACGTT
TCAGAGCTACATTATTCCGACAAACAATGTTCGCAGAATTTGAGCATAAAATTCATGCAATTGAAGAAGCAGGTGAACCA
TTAACGCCAACTAGAATGAATGAAGAATATGCCAAATTAAATAAATTATACTTCGGTGATTCTGTAGAAACTGATGAAGA
TATTAGTAAGGAATGGTCACGTATTCCACACTTCTATATGAATTATTATGTATATCAATACGCAACTGGTTACAGTGCAG
CTCAAAGCTTAAGTCATCAAATTTTAACAGAAGGTAAGCCAGCAGTAGATAGATATATTAATGAATTCTTGAAAAAAGGT
AGCTCAAATTATCCAATTGAGATATTAAAAAATGCTGGTGTAGATATGACAACACCTGAACCAATTGAACAAGCTTGTGA
AGTTTTTGAACAAAAATTGAACGCTTTTGAAAAATTAATGAAAGCTTAG

Domains

Predicted by InterProScan.

Oligopeptidase F (Peptidase_M3_N)

(117-186)

Peptidase family M3 (Peptidase_M3)

(208-587)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2XIW1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

43.782

97.508

0.427

Multiple sequence alignment