Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AAVC70_RS12565 Genome accession   NZ_CP154898
Coordinates   2240426..2240863 (-) Length   145 a.a.
NCBI ID   WP_013350968.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain Fad 97     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2235426..2245863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAVC70_RS12540 (AAVC70_12540) - 2236448..2237579 (+) 1132 Protein_2312 zinc-dependent alcohol dehydrogenase -
  AAVC70_RS12545 (AAVC70_12545) - 2237594..2238028 (+) 435 WP_013350964.1 RDD family protein -
  AAVC70_RS12550 (AAVC70_12550) - 2238102..2238425 (+) 324 WP_013350965.1 YckD family protein -
  AAVC70_RS12555 (AAVC70_12555) - 2238530..2239966 (+) 1437 WP_013350966.1 family 1 glycosylhydrolase -
  AAVC70_RS12560 (AAVC70_12560) nin/comJ 2240007..2240405 (-) 399 WP_061573682.1 competence protein ComJ Regulator
  AAVC70_RS12565 (AAVC70_12565) nucA/comI 2240426..2240863 (-) 438 WP_013350968.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  AAVC70_RS12570 (AAVC70_12570) hxlB 2241214..2241771 (-) 558 WP_013350969.1 6-phospho-3-hexuloisomerase -
  AAVC70_RS12575 (AAVC70_12575) hxlA 2241768..2242403 (-) 636 WP_013350970.1 3-hexulose-6-phosphate synthase -
  AAVC70_RS12580 (AAVC70_12580) - 2242635..2242997 (+) 363 WP_013350971.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=994864 AAVC70_RS12565 WP_013350968.1 2240426..2240863(-) (nucA/comI) [Bacillus amyloliquefaciens strain Fad 97]
MKTLKALLLVLIIIAGAVAALIKGDIFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=994864 AAVC70_RS12565 WP_013350968.1 2240426..2240863(-) (nucA/comI) [Bacillus amyloliquefaciens strain Fad 97]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATATTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAACAATCTCTTAAACATGTGCCTGTAAAAACAGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCAGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment