Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   AAVB98_RS03285 Genome accession   NZ_CP154895
Coordinates   622932..626432 (-) Length   1166 a.a.
NCBI ID   WP_013351760.1    Uniprot ID   A0A9P1JFY3
Organism   Bacillus amyloliquefaciens strain Fad 77     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 617932..631432
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAVB98_RS03275 (AAVB98_03275) sbcD 618002..619174 (-) 1173 WP_013351762.1 exonuclease subunit SbcD -
  AAVB98_RS03280 (AAVB98_03280) addA 619241..622945 (-) 3705 WP_041481719.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AAVB98_RS03285 (AAVB98_03285) addB 622932..626432 (-) 3501 WP_013351760.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AAVB98_RS03290 (AAVB98_03290) - 626573..627286 (+) 714 WP_013351759.1 DUF421 domain-containing protein -
  AAVB98_RS03295 (AAVB98_03295) - 627355..627882 (+) 528 WP_013351758.1 ferritin-like domain-containing protein -
  AAVB98_RS03300 (AAVB98_03300) - 628123..628743 (+) 621 WP_013351757.1 TetR/AcrR family transcriptional regulator -
  AAVB98_RS03305 (AAVB98_03305) - 628759..629955 (+) 1197 WP_013351756.1 MFS transporter -
  AAVB98_RS03310 (AAVB98_03310) - 630121..631278 (+) 1158 WP_013351755.1 AbrB family transcriptional regulator -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 133648.23 Da        Isoelectric Point: 5.3147

>NTDB_id=994757 AAVB98_RS03285 WP_013351760.1 622932..626432(-) (addB) [Bacillus amyloliquefaciens strain Fad 77]
MGVEFLVGRSGSGKTRLIIDSIQEELRREPFGKPIIFLVPDQMTFLMEYELAKTPDIGGMIRAQVFSFSRLAWRVLQHTG
GMNRPFVTTTGVQMLLRKLIEEHKHDFKVYQKASDKTGFTEQVERMLTEFKRYCIEPEDVRRMAESGTASEYRGERMLSE
KLHDLGILYQQMERSLAGHYLHSEDYLTLLAQQIPLADVIKGARVYVDGFYQFTPQELRVLEQLIMHAEHVTFSLTADSP
SAEQAPDELDLFRMTGSTYYKLYQTAKELNADISCKELHGTKRHQHVPELACIESQYDVRPAAVYTGGQEAFTVIQAQNR
RAELEGIAREIQSLVRDRGYRYRDMAILIRQPEDYKDLLKEVFADYGLPYFIDGKASMQHHPLIEFIRSSLDVVKGNWRY
EAVFRCAKTELLFPLDQPEQKIREQVDQLENYCIAYGIKGERWTNGERFVYRRFVSLDEDFAQTDQEIEMEHMLNETREW
MAEPLIRLQNRLKKAKTVQSMAEALYLFLEDTDVPLKLDRKRQQAEEAGNMVEAQQHGQAWDAVIQLLEEFAGMMGEDEM
SLALFQQMLETGTESLHFSLIPPALDQVFVGNMDLSRMYGTSCTFVIGANDGVLPARPDENGVLSDDDREWLKAVGVELS
SAGRERLLDEHFLIYMALSSPSDRLYVTYPIADAEGKTLLPSIVVNRLGELFPDHQEKLSAADPEQVSEEEQLQYLVNKQ
VAQTYTASQLRLWTREYEISDVWWSAYNVLMKEPDHERAKKLFSSLFFRNEAKRLDRPVSRQLYGEHIKGSVSRMEAFNA
CQFSHFASHGLQLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLEWRDLTKDQCRNFSYDAVERLAPRLQKEILLSSN
RHFYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGSGPLPPLTFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMKATPAGVLYFHIHDPMIQASLPMELDEIEQEIFKKFKMKGL
LLGDREAISLMDTTLEEGRSNIINAGLKKDGSLRSDSAAVSERDFHLLTDHVRRTFEQAGEAITDGLVSITPYKLKDKTP
CTYCAFKSVCQFDESLKENEYRSLKAEKDGTILDWLKKEANDDANS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=994757 AAVB98_RS03285 WP_013351760.1 622932..626432(-) (addB) [Bacillus amyloliquefaciens strain Fad 77]
TTGGGTGTTGAGTTTTTAGTAGGCAGATCGGGGAGTGGGAAAACGAGGCTGATTATCGACAGCATTCAAGAAGAACTGCG
CCGGGAGCCGTTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCGGATATCGGCGGCATGATACGCGCTCAAGTGTTCAGCTTTTCGCGTTTGGCGTGGCGCGTGCTTCAGCACACGGGG
GGCATGAACCGTCCGTTTGTGACGACGACGGGTGTGCAGATGCTTTTAAGGAAACTGATTGAAGAACATAAGCACGATTT
TAAAGTCTATCAAAAAGCGAGTGACAAAACCGGATTCACTGAGCAGGTGGAACGGATGCTGACAGAGTTTAAGCGGTACT
GTATTGAACCTGAAGACGTCCGCCGCATGGCAGAAAGCGGAACGGCGTCAGAATACCGCGGAGAGCGGATGCTGTCTGAA
AAACTTCATGACTTAGGCATTTTATATCAGCAGATGGAAAGAAGCCTCGCCGGCCACTATTTGCATTCAGAGGATTATTT
GACGCTGCTCGCTCAGCAGATTCCTTTGGCGGATGTTATAAAAGGCGCCCGTGTTTATGTTGACGGCTTCTATCAATTTA
CGCCGCAGGAATTAAGGGTGCTGGAGCAGCTGATCATGCATGCTGAACATGTCACATTCTCTCTGACGGCGGACAGCCCT
TCGGCGGAACAGGCTCCGGATGAGCTCGATCTGTTCAGAATGACCGGCAGCACATACTATAAGCTTTATCAGACGGCAAA
GGAGTTGAATGCCGATATTTCCTGCAAGGAATTGCATGGAACGAAGCGGCATCAGCATGTGCCGGAGCTTGCCTGTATAG
AATCGCAGTACGATGTGCGTCCCGCGGCTGTGTATACGGGCGGACAGGAAGCTTTTACCGTCATACAGGCACAGAACAGA
AGAGCTGAACTCGAGGGTATTGCCCGGGAAATTCAATCACTTGTCCGGGACAGAGGATACCGGTACAGAGATATGGCGAT
TCTCATCCGTCAGCCGGAAGATTATAAAGACTTGCTGAAGGAAGTGTTTGCAGATTACGGGCTTCCTTATTTTATAGACG
GAAAAGCTTCTATGCAGCATCACCCGCTTATTGAATTCATCCGCTCAAGCTTGGATGTCGTGAAGGGGAATTGGAGATAT
GAAGCGGTGTTCCGCTGTGCGAAAACTGAACTGTTATTCCCCCTTGATCAGCCGGAGCAGAAAATAAGAGAGCAGGTTGA
TCAGCTTGAAAACTACTGTATCGCCTACGGAATCAAAGGCGAGCGCTGGACGAACGGGGAGCGGTTTGTATACAGGCGGT
TCGTATCGCTCGATGAGGATTTCGCGCAGACGGATCAGGAAATAGAGATGGAACACATGCTGAATGAGACGAGAGAGTGG
ATGGCCGAGCCGCTCATCCGGCTTCAAAACCGGCTGAAAAAAGCGAAAACGGTTCAAAGCATGGCGGAAGCTCTTTATCT
CTTTTTGGAAGATACCGATGTTCCCCTGAAGCTGGACCGGAAAAGACAGCAGGCCGAAGAGGCCGGAAACATGGTTGAAG
CGCAGCAGCACGGCCAGGCGTGGGATGCCGTTATCCAGCTTCTCGAAGAGTTTGCGGGCATGATGGGTGAAGATGAAATG
TCTTTGGCGCTGTTCCAGCAAATGCTTGAAACGGGAACCGAATCGCTTCATTTTTCTTTAATTCCTCCTGCGCTTGATCA
AGTATTTGTAGGGAATATGGATCTGTCCAGAATGTACGGCACATCCTGCACATTTGTCATCGGGGCCAATGACGGCGTTC
TTCCGGCGCGCCCGGATGAAAACGGCGTGCTGTCTGATGATGACCGGGAGTGGCTGAAAGCGGTCGGAGTGGAGCTTTCT
TCCGCGGGGCGTGAGCGGCTGCTTGATGAGCATTTTCTCATCTATATGGCGCTGTCAAGTCCGTCTGACCGTCTGTATGT
GACATACCCGATTGCCGATGCGGAAGGAAAAACGCTCCTGCCGTCAATCGTTGTAAACAGGCTCGGCGAGCTGTTTCCCG
ATCACCAGGAAAAGCTGTCAGCGGCTGATCCCGAGCAGGTAAGCGAAGAAGAACAGCTTCAGTATCTCGTCAATAAACAG
GTGGCACAGACGTATACGGCAAGCCAGCTCAGGCTGTGGACAAGAGAGTACGAGATCAGTGACGTGTGGTGGAGCGCATA
CAATGTGCTGATGAAAGAGCCTGATCACGAGCGTGCGAAAAAGCTGTTTTCAAGCCTGTTTTTCCGAAATGAGGCAAAGC
GTCTTGACCGGCCTGTTTCGCGCCAGCTTTACGGCGAGCACATTAAAGGAAGCGTCTCTCGTATGGAGGCGTTTAATGCC
TGCCAGTTTTCTCATTTTGCATCTCACGGGCTGCAATTAAAAGAGCGGCAGTTTTTCAAGCTGGAAGCGCCGGATATCGG
ACAGCTGTTTCACTCCAGCCTGAAGCTGATTTCAGACCGGCTTCGTGAGCAGAAGCTGGAATGGCGCGATCTGACGAAAG
ATCAGTGCCGGAATTTTTCCTATGATGCGGTGGAACGGCTTGCGCCAAGGCTGCAAAAAGAGATTCTGCTCAGCTCAAAC
AGGCACTTTTATGTGAAGGAAAAACTGCAGAAGATCGTCACGCGTGTCTCAGGCATATTAAGCGAGCATGCGAAGGCGAG
CGGTTTTGTCCCGATCGGCCTTGAACTCGGTTTCGGCGGAAGCGGACCGCTGCCTCCGCTCACTTTCACGCTGAAAAACG
GCTGTACGATGGAACTCGTCGGCCGGATCGACCGCGTGGATAAAGCGGAAAGCTCAAAAGGCCTGCTGCTGAGAATCGTT
GATTATAAATCAAGCGACAAGGGGCTTGATCTCGCTGAGGTATATTACGGGCTCGCTCTGCAAATGCTCACATACCTTGA
CTTATCAATTACACACTCAGCGGATTGGCTCGGCATGAAAGCGACACCGGCAGGCGTTTTGTATTTCCATATTCATGATC
CGATGATTCAGGCCAGTCTGCCGATGGAACTTGATGAAATCGAGCAGGAAATTTTTAAGAAATTTAAAATGAAAGGCCTT
CTCCTCGGTGACCGGGAGGCGATCAGCCTGATGGACACGACTTTGGAGGAAGGGCGTTCAAATATTATCAATGCCGGTTT
GAAAAAGGACGGATCTTTACGGTCCGATTCAGCGGCGGTCAGCGAACGGGATTTTCATCTGCTGACAGACCATGTGAGGC
GGACATTTGAGCAGGCGGGCGAAGCGATAACGGACGGGCTTGTGTCCATTACCCCGTATAAGCTGAAAGATAAGACGCCT
TGTACGTACTGTGCTTTTAAATCGGTGTGCCAGTTTGATGAGTCGCTTAAAGAAAATGAATACCGCAGCCTGAAAGCGGA
AAAAGACGGCACTATACTGGATTGGCTGAAAAAGGAGGCGAATGATGATGCAAATTCCTAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(307-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(791-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

82.933

100

0.829

Multiple sequence alignment