Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AAVD18_RS05325 Genome accession   NZ_CP154893
Coordinates   1022774..1023145 (+) Length   123 a.a.
NCBI ID   WP_232469737.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain Fad 11/2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1017774..1028145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAVD18_RS05320 (AAVD18_05320) - 1021210..1022421 (-) 1212 WP_013352340.1 cytochrome P450 -
  AAVD18_RS05325 (AAVD18_05325) nucA/comI 1022774..1023145 (+) 372 WP_232469737.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  AAVD18_RS05330 (AAVD18_05330) - 1023229..1023960 (+) 732 WP_013352342.1 YoaK family protein -
  AAVD18_RS05335 (AAVD18_05335) - 1023995..1024357 (-) 363 WP_013352343.1 hypothetical protein -
  AAVD18_RS05340 (AAVD18_05340) - 1024550..1025878 (-) 1329 WP_013352344.1 S8 family peptidase -
  AAVD18_RS05345 (AAVD18_05345) - 1026062..1026295 (+) 234 WP_014471844.1 hypothetical protein -
  AAVD18_RS05350 (AAVD18_05350) - 1026550..1027257 (+) 708 WP_013352345.1 poly-gamma-glutamate hydrolase family protein -
  AAVD18_RS05355 (AAVD18_05355) - 1027317..1027769 (+) 453 WP_013352346.1 OsmC family protein -
  AAVD18_RS05360 (AAVD18_05360) - 1027783..1028136 (-) 354 WP_014470379.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 123 a.a.        Molecular weight: 13302.74 Da        Isoelectric Point: 5.5837

>NTDB_id=994689 AAVD18_RS05325 WP_232469737.1 1022774..1023145(+) (nucA/comI) [Bacillus amyloliquefaciens strain Fad 11/2]
MGAGIHSENTAEAASRYDHVLYFPLLKYPETGNHIKDAISAGHSDICTIDRGGAEERRKESLKGIPTKPGFDRDEWPMAV
CTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 372 bp        

>NTDB_id=994689 AAVD18_RS05325 WP_232469737.1 1022774..1023145(+) (nucA/comI) [Bacillus amyloliquefaciens strain Fad 11/2]
ATGGGCGCCGGCATCCATTCGGAGAATACTGCTGAAGCTGCATCCCGATATGATCATGTGCTGTATTTCCCGCTGTTAAA
ATATCCGGAGACGGGGAATCATATAAAAGATGCCATTTCGGCAGGGCATTCTGACATTTGTACAATTGATCGCGGCGGAG
CTGAGGAAAGAAGGAAAGAATCATTGAAAGGCATTCCGACAAAGCCTGGGTTTGACCGTGATGAATGGCCGATGGCAGTC
TGTACGGAAGGCGGGGCAGGGGCTGACATCAGATATGTAACCCCTTCGGATAATCGCGGAGCCGGTTCATGGGTTGGAAA
TCAAATGAGCGGATATTCCGACGGTACGAGAGTATTATTTATCGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(29-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.557

99.187

0.561

Multiple sequence alignment