Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   EW022_RS05645 Genome accession   NZ_LR130240
Coordinates   1105823..1108033 (-) Length   736 a.a.
NCBI ID   WP_011284724.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain PS006 isolate PS006     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1100823..1113033
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW022_RS05615 - 1101056..1102162 (-) 1107 WP_011284727.1 NAD(P)/FAD-dependent oxidoreductase -
  EW022_RS05620 - 1102202..1102990 (-) 789 WP_021340967.1 Nif3-like dinuclear metal center hexameric protein -
  EW022_RS05625 - 1102980..1103666 (-) 687 WP_011284725.1 tRNA (adenine(22)-N(1))-methyltransferase -
  EW022_RS05630 nth 1103738..1104394 (-) 657 WP_002990106.1 endonuclease III -
  EW022_RS05635 - 1104391..1105074 (-) 684 WP_002984890.1 DnaD domain-containing protein -
  EW022_RS05640 - 1105155..1105673 (-) 519 WP_002990109.1 adenine phosphoribosyltransferase -
  EW022_RS05645 recJ 1105823..1108033 (-) 2211 WP_011284724.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  EW022_RS05650 - 1108030..1108794 (-) 765 WP_002990111.1 SDR family NAD(P)-dependent oxidoreductase -
  EW022_RS05655 rnz 1108794..1109723 (-) 930 WP_009881223.1 ribonuclease Z -
  EW022_RS05660 - 1109738..1110373 (-) 636 WP_002990114.1 cystathionine beta-lyase -
  EW022_RS05665 hflX 1110366..1111604 (-) 1239 WP_010922191.1 GTPase HflX -
  EW022_RS05670 miaA 1111677..1112576 (-) 900 WP_011284721.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
  EW022_RS05675 - 1112710..1112880 (+) 171 WP_011284720.1 DUF3042 family protein -

Sequence

Protein


Download         Length: 736 a.a.        Molecular weight: 82579.45 Da        Isoelectric Point: 4.7862

>NTDB_id=994528 EW022_RS05645 WP_011284724.1 1105823..1108033(-) (recJ) [Streptococcus pyogenes strain PS006 isolate PS006]
MIKSKYSWKIKDKKPDDGFFKLAKTKGLTQTAAQLIYERGIRTEEALDEFLTADLSQLHDPYLLHDMAKAVPRIRQAIEE
GERILIYGDYDADGMTSASIVKETLDMMGAEPLVYLPNRFTDGYGPNQSVYKYFIEQEAVSLIITVDNGVAGHEAIRYAQ
EQEVDVIVTDHHSLPEELPEAFAIIHPEHPDADYPFKHLAGCGVAFKLATALLESLPTDCLDLVAIGTIADMVSLTGENR
VLVKNGLAMLKHTERVGLQELMSLSPIDLEHFNEDAIGFQIAPQLNALGRLDDPNPAIELLTGFDDQEAQAIALMIKKKN
EERKALVQDIFDQAMAMVDPQKPVQVLAQAGWHPGVLGIVAGRIMETIGQTVVVLTIDNGFAKGSARSLEAINIFEALNG
KRELFTAFGGHAGAAGMTLPVDNLEALSDFLCQFVIERGLDQTAKNTLTIDERLSLDDLSLDILKSLDKLAPYGMDHQKP
VFYVKDIRVSQARTIGQDQSHLKFKVSQGKASFDVLAFGQGSQLQEFRQATGLELAVTLSVNHWNGNTSLQFMLVDARVD
GVQLLDLRTKTAKVPEGIPTIEEDPNARVILINDIPEDFKTWRNQFVHKDFDAIYFKNQMKHPYYLTGFGSREQFAKLYK
TIYQFPEFDLRHKLTELSHYLNIEKLLLIKLIQIFEELSFVTIDDGLMTVNPQAQKREISESHIYQDLKELVKFQEIMAL
ASPKEMYDYLVKSNDQ

Nucleotide


Download         Length: 2211 bp        

>NTDB_id=994528 EW022_RS05645 WP_011284724.1 1105823..1108033(-) (recJ) [Streptococcus pyogenes strain PS006 isolate PS006]
ATGATTAAATCCAAATATAGCTGGAAAATAAAAGACAAGAAGCCAGATGATGGCTTCTTTAAGCTAGCAAAGACAAAAGG
TCTAACCCAGACCGCCGCTCAGTTGATTTATGAGCGAGGGATAAGGACTGAAGAAGCCTTAGATGAGTTTTTAACAGCGG
ACTTGTCTCAACTGCATGATCCTTACTTGCTACATGACATGGCAAAAGCTGTGCCAAGAATTCGTCAAGCTATTGAAGAG
GGCGAACGAATTCTCATTTACGGCGACTATGATGCTGATGGCATGACGTCGGCTTCTATTGTCAAAGAAACCCTAGACAT
GATGGGAGCAGAGCCTTTGGTTTATCTTCCCAACCGCTTTACAGATGGCTATGGCCCCAACCAGAGCGTCTATAAATATT
TTATTGAGCAGGAAGCTGTGTCCCTAATTATCACGGTTGATAATGGCGTGGCCGGACATGAAGCGATTAGATATGCTCAA
GAACAGGAAGTTGATGTCATTGTAACTGACCATCATAGCCTGCCTGAAGAATTGCCAGAAGCCTTTGCCATCATTCATCC
TGAGCATCCTGATGCTGATTATCCCTTTAAACACTTAGCTGGCTGTGGCGTTGCCTTTAAATTGGCAACAGCTTTGCTAG
AAAGTTTACCAACAGACTGTCTAGACTTGGTAGCTATTGGAACCATTGCCGATATGGTAAGCTTGACAGGTGAAAACCGT
GTTTTAGTCAAAAATGGTCTGGCCATGCTCAAACACACCGAAAGAGTGGGCTTGCAGGAGTTGATGTCTTTGTCTCCTAT
TGATTTGGAACATTTTAATGAAGACGCTATTGGCTTTCAGATTGCTCCGCAATTAAATGCTTTAGGGCGCTTAGATGACC
CTAATCCAGCCATTGAGCTACTGACGGGCTTTGATGACCAAGAAGCTCAAGCGATTGCCTTGATGATTAAGAAAAAAAAT
GAAGAGCGTAAGGCCTTAGTCCAAGACATTTTTGATCAAGCGATGGCTATGGTTGATCCTCAAAAACCTGTTCAAGTCTT
GGCACAAGCTGGCTGGCATCCTGGTGTTCTTGGGATTGTCGCTGGTCGTATCATGGAAACTATTGGACAAACGGTCGTCG
TTTTGACCATTGATAATGGCTTTGCAAAAGGCTCGGCCAGAAGTTTAGAAGCTATTAATATTTTTGAAGCCTTGAATGGC
AAGCGTGAGTTATTTACAGCCTTTGGGGGACATGCTGGTGCAGCAGGAATGACCCTGCCCGTTGACAACTTGGAAGCTCT
TTCAGACTTTCTTTGTCAGTTTGTGATAGAACGTGGCTTAGATCAGACTGCCAAAAATACGTTAACTATTGATGAAAGAC
TGTCTTTGGACGACCTTAGCCTTGATATTTTAAAGAGTTTAGATAAATTAGCCCCTTATGGGATGGATCATCAAAAGCCG
GTCTTTTATGTCAAGGACATAAGGGTTAGTCAAGCGCGTACCATAGGGCAAGACCAGAGCCATCTTAAATTTAAAGTCAG
TCAGGGCAAAGCTTCCTTTGACGTACTGGCTTTTGGTCAAGGAAGTCAACTGCAAGAGTTCCGACAAGCTACTGGTTTAG
AATTAGCAGTTACCTTATCTGTCAACCATTGGAATGGGAACACGAGTCTCCAATTCATGTTAGTAGATGCTCGTGTTGAC
GGAGTGCAACTTCTTGACTTAAGGACAAAAACAGCCAAAGTTCCTGAAGGTATTCCGACTATTGAAGAAGACCCAAATGC
CAGAGTCATTCTGATTAATGACATCCCTGAAGATTTCAAAACCTGGCGCAACCAGTTTGTTCACAAGGATTTTGACGCCA
TCTATTTTAAAAATCAGATGAAACATCCTTATTATTTAACAGGATTTGGCAGCAGAGAGCAGTTTGCCAAGCTCTACAAA
ACCATCTATCAGTTTCCTGAATTTGATTTACGGCATAAATTGACAGAGTTGAGTCACTATTTAAACATAGAAAAACTTTT
ACTGATTAAGTTGATTCAAATTTTTGAAGAGTTGTCTTTTGTGACGATTGACGATGGGCTGATGACGGTTAACCCCCAAG
CGCAAAAACGAGAGATTTCAGAGAGCCACATTTACCAAGACTTAAAAGAATTGGTGAAATTTCAAGAAATCATGGCTTTG
GCTAGCCCGAAAGAGATGTATGATTATTTGGTGAAATCAAACGATCAATAA

Domains

Predicted by InterProScan.

RecJ OB domain (RecJ_OB)

(450-556)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(588-729)

DHHA1 domain (DHHA1)

(317-435)

DHH family (DHH)

(83-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Streptococcus pneumoniae strain D39V

63.144

100

0.633
  recJ Bacillus subtilis subsp. subtilis str. 168

34.866

100

0.371