Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AAG895_RS10765 Genome accession   NZ_CP154859
Coordinates   2412592..2414004 (-) Length   470 a.a.
NCBI ID   WP_345792016.1    Uniprot ID   -
Organism   Thauera sp. JM12B12     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2407592..2419004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG895_RS10745 (AAG895_10745) - 2409200..2409949 (+) 750 WP_345792012.1 2Fe-2S iron-sulfur cluster-binding protein -
  AAG895_RS10750 (AAG895_10750) - 2409949..2410539 (+) 591 WP_345792013.1 NADP oxidoreductase -
  AAG895_RS10755 (AAG895_10755) - 2410550..2412016 (+) 1467 WP_345792014.1 Ni/Fe hydrogenase subunit alpha -
  AAG895_RS10760 (AAG895_10760) - 2412013..2412459 (+) 447 WP_345792015.1 hydrogenase maturation protease -
  AAG895_RS10765 (AAG895_10765) radA 2412592..2414004 (-) 1413 WP_345792016.1 DNA repair protein RadA Machinery gene
  AAG895_RS10770 (AAG895_10770) - 2414080..2414991 (-) 912 Protein_2117 LysR family transcriptional regulator -
  AAG895_RS10775 (AAG895_10775) lplT 2415220..2416470 (+) 1251 WP_345792017.1 lysophospholipid transporter LplT -
  AAG895_RS10780 (AAG895_10780) - 2416564..2417472 (-) 909 WP_345792018.1 uracil-DNA glycosylase -
  AAG895_RS10785 (AAG895_10785) rimI 2417472..2417918 (-) 447 WP_345792019.1 ribosomal protein S18-alanine N-acetyltransferase -
  AAG895_RS10790 (AAG895_10790) tsaB 2417918..2418610 (-) 693 WP_345792020.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB -

Sequence

Protein


Download         Length: 470 a.a.        Molecular weight: 49819.18 Da        Isoelectric Point: 7.6698

>NTDB_id=994154 AAG895_RS10765 WP_345792016.1 2412592..2414004(-) (radA) [Thauera sp. JM12B12]
MARSKSAFVCTECGAQAVRWQGQCPQCQAWNTMVETVLEPSAPAAGSRFAALAGETSRLQPLSALEPREEPRQPTGLDEF
DRVLGGGLVAGGVVLIGGDPGIGKSTLLLQALSHLAGSRPAAGGEGAAAKAAAGVIYVSGEESGEQVALRAQRLQLPSSP
LQMLAEINLERILHTLRAARPRVAVIDSIQTVYSETLQSAPGSVAQVRECAAQLTRFAKQSGTSLILVGHVTKDGTLAGP
RVLEHIVDTVLYFEGDTHSSFRLIRAFKNRFGAVNELGVFAMTERGLRGVSNPSAIFLSQHSQQVAGSCVLITQEGTRPL
LVEVQALVDSSQSPNPRRLSVGLEQNRLAMLLAVMHRHAGVVCFDQDVFVNAVGGVKITEPAADLAILFAIVSSLRDRPL
KRGLAVFGEVGLAGEIRPAPRGQERLKEAAKLGFDTAIVPRANAPKQPIEGLTVIAVERVEEALEQLRGL

Nucleotide


Download         Length: 1413 bp        

>NTDB_id=994154 AAG895_RS10765 WP_345792016.1 2412592..2414004(-) (radA) [Thauera sp. JM12B12]
ATGGCACGCTCGAAATCCGCTTTCGTCTGCACCGAATGCGGTGCGCAGGCCGTGCGCTGGCAAGGCCAGTGCCCGCAATG
CCAGGCCTGGAATACCATGGTCGAGACGGTACTGGAGCCGTCCGCGCCCGCAGCAGGTAGTCGCTTCGCCGCATTGGCGG
GCGAAACCAGCCGCTTGCAGCCCTTGTCCGCACTCGAACCCCGCGAGGAACCGCGTCAGCCCACCGGCCTGGACGAGTTC
GACCGCGTGCTCGGCGGCGGCCTGGTCGCCGGCGGCGTGGTGCTGATCGGTGGCGACCCGGGGATCGGCAAGTCGACGCT
GCTGCTGCAGGCCTTGTCCCATCTTGCAGGATCGCGTCCGGCCGCGGGCGGAGAGGGCGCGGCCGCCAAGGCGGCTGCAG
GGGTGATCTACGTCAGCGGCGAGGAGTCGGGCGAGCAGGTCGCGCTGCGCGCCCAGCGCCTGCAACTGCCATCGTCGCCG
CTGCAGATGCTGGCCGAGATCAACCTCGAGCGCATCCTGCACACGCTGCGCGCGGCGCGCCCGCGGGTCGCGGTCATCGA
CTCGATCCAGACCGTGTATTCCGAGACCCTGCAGTCCGCCCCCGGCTCGGTCGCCCAGGTGCGCGAATGCGCCGCGCAGC
TCACCCGCTTCGCCAAGCAGAGCGGCACCAGCCTGATCCTGGTCGGCCACGTCACCAAGGATGGCACGCTCGCCGGTCCG
CGCGTGCTTGAGCACATCGTCGACACCGTGCTCTATTTCGAGGGCGACACCCACTCCAGCTTCCGGCTGATCCGGGCGTT
CAAGAACCGCTTCGGCGCGGTCAACGAGCTCGGCGTGTTCGCGATGACCGAGCGCGGCCTGCGCGGCGTCTCCAATCCCT
CGGCGATCTTCCTGTCGCAGCACTCGCAGCAGGTCGCCGGCAGCTGTGTGCTGATCACCCAGGAAGGCACCCGCCCGCTG
CTGGTCGAGGTGCAGGCCCTGGTCGATTCATCGCAGAGCCCCAATCCGCGCCGGCTGTCGGTCGGCCTGGAGCAAAACCG
GCTGGCCATGCTGCTTGCGGTGATGCACCGCCACGCCGGTGTGGTGTGCTTCGATCAGGACGTGTTCGTCAATGCCGTCG
GCGGGGTCAAGATCACCGAGCCGGCGGCCGACCTCGCGATCCTGTTCGCGATCGTGTCCTCGCTGCGCGACCGTCCGCTC
AAGCGCGGGCTAGCGGTGTTCGGCGAGGTCGGCCTTGCCGGCGAGATCCGCCCCGCGCCGCGCGGGCAGGAACGGCTGAA
GGAAGCGGCCAAGCTCGGCTTCGACACCGCGATCGTTCCGCGCGCGAACGCGCCCAAGCAGCCGATCGAGGGACTCACCG
TGATCGCGGTCGAGCGGGTGGAGGAGGCGCTGGAGCAGTTGCGCGGCTTGTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(83-235)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(374-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.467

99.787

0.494
  radA Streptococcus pneumoniae Rx1

43.552

100

0.438
  radA Streptococcus pneumoniae R6

43.552

100

0.438
  radA Streptococcus pneumoniae TIGR4

43.552

100

0.438
  radA Streptococcus pneumoniae D39

43.552

100

0.438
  radA Streptococcus mitis SK321

43.71

99.787

0.436
  radA Streptococcus mitis NCTC 12261

43.59

99.574

0.434

Multiple sequence alignment