Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EQB41_RS00405 Genome accession   NZ_LR129840
Coordinates   61145..62506 (+) Length   453 a.a.
NCBI ID   WP_078375446.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 4496 isolate 4496     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 56145..67506
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQB41_RS00355 - 56231..56434 (+) 204 WP_001091109.1 hypothetical protein -
  EQB41_RS00360 - 56437..56787 (+) 351 WP_000852247.1 hypothetical protein -
  EQB41_RS00365 - 56797..57213 (+) 417 WP_001165344.1 phage holin family protein -
  EQB41_RS00370 - 57217..57549 (+) 333 WP_001186238.1 phage holin -
  EQB41_RS00375 - 57553..58509 (+) 957 WP_127820689.1 N-acetylmuramoyl-L-alanine amidase family protein -
  EQB41_RS00380 - 58778..58957 (-) 180 WP_001233269.1 hypothetical protein -
  EQB41_RS11725 - 59099..59248 (-) 150 WP_162489709.1 hypothetical protein -
  EQB41_RS00385 tadA 59518..59985 (+) 468 WP_000291870.1 tRNA adenosine(34) deaminase TadA -
  EQB41_RS00395 - 60171..60614 (+) 444 WP_000701992.1 dUTP diphosphatase -
  EQB41_RS00400 - 60616..61131 (+) 516 WP_001836031.1 histidine phosphatase family protein -
  EQB41_RS00405 radA 61145..62506 (+) 1362 WP_078375446.1 DNA repair protein RadA Machinery gene
  EQB41_RS00410 - 62579..63076 (+) 498 WP_001809263.1 beta-class carbonic anhydrase -
  EQB41_RS00415 - 63097..63912 (+) 816 WP_050234444.1 PrsW family intramembrane metalloprotease -
  EQB41_RS00420 - 64057..65025 (+) 969 WP_054377095.1 ribose-phosphate diphosphokinase -
  EQB41_RS00425 - 65159..65440 (-) 282 Protein_80 ISL3 family transposase -
  EQB41_RS12130 - 65567..66474 (-) 908 Protein_81 Rpn family recombination-promoting nuclease/putative transposase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49388.90 Da        Isoelectric Point: 6.5129

>NTDB_id=993614 EQB41_RS00405 WP_078375446.1 61145..62506(+) (radA) [Streptococcus pneumoniae strain 4496 isolate 4496]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLAGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPQNSLTGITLPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=993614 EQB41_RS00405 WP_078375446.1 61145..62506(+) (radA) [Streptococcus pneumoniae strain 4496 isolate 4496]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGCCCCAACTG
TGGGTCTTGGTCTTCTTTTGTGGAAGAGGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTGTCCTTGACAGGTGAGAAAA
CCAAGCCCATGAAACTAGCTGAGGTGACTTCCATCAATGTCAATCGAACCAAGACGGAGATGGAGGAATTCAACCGTGTG
CTTGGAGGCGGAGTGGTACCAGGAAGTCTCGTCCTCATCGGTGGGGATCCTGGGATTGGGAAATCAACTCTTCTCCTACA
AGTCTCAACCCAGTTGTCCCAAGTGGGGACAGTTCTCTATGTCAGTGGGGAGGAGTCTGCCCAGCAGATTAAACTACGTG
CAGAGCGCTTAGGTGATATTGATAGTGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGTGCAGAAGTGGAG
CGTATCCAGCCAGACTTTCTTATTATTGATTCCATCCAGACCATCATGTCTCCTGAGATTTCAGGGGTGCAGGGGTCTGT
TTCTCAGGTACGTGAAGTGACCGCTGAACTCATGCAGTTGGCCAAGACCAATAACATTGCCATCTTTATCGTAGGTCATG
TGACCAAAGAAGGAACCTTGGCTGGGCCTCGTATGTTGGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAGCGT
CACCACACCTTTCGTATTTTGAGAGCGGTCAAAAATCGTTTTGGTTCCACTAATGAGATTGGGATTTTTGAGATGCAGTC
GGGCGGCTTGGTTGAGGTACTCAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGCTGGGGCGACTGGTTCCTCCATCG
TTGTAACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCTTTGGTAACACCGACCATGTTTGGAAATGCCAAG
CGTACTACGACAGGACTTGATTTTAACCGTGCTAGCTTGATTATGGCTGTTTTGGAAAAACGGGCAGGGCTTCTCTTGCA
AAATCAGGATGCCTATCTCAAATCTGCTGGTGGTGTTAAATTGGATGAACCTGCCATTGACTTGGCTGTTGCAGTTGCTA
TTGCTTCGAGCTACAAAGACAAGCCAACTAATCCTCAGGAATGTTTTGTCGGAGAACTGGGCTTGACAGGAGAGATTCGG
CGCGTGAATCGTATTGAGCAACGCATCAACGAAGCTGCTAAACTGGGCTTTACTAAGATTTATGTACCTCAGAATTCCTT
GACAGGAATCACTCTGCCTAAGGAAATTCAGGTCATTGGCGTGACAACGATTCAGGAAGTCTTGAAAAAGGTCTTTGCAT
AA

Domains

Predicted by InterProScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(63-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

99.558

100

0.996
  radA Streptococcus pneumoniae D39

99.558

100

0.996
  radA Streptococcus pneumoniae R6

99.558

100

0.996
  radA Streptococcus pneumoniae TIGR4

99.558

100

0.996
  radA Streptococcus mitis NCTC 12261

99.117

100

0.991
  radA Streptococcus mitis SK321

99.117

100

0.991
  radA/sms Bacillus subtilis subsp. subtilis str. 168

63.355

100

0.634

Multiple sequence alignment