Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AAHF05_RS20060 Genome accession   NZ_CP154353
Coordinates   4123267..4124700 (+) Length   477 a.a.
NCBI ID   WP_129324191.1    Uniprot ID   -
Organism   Enterobacter adelaidei strain ECC3473     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4118267..4129700
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAHF05_RS20030 (AAHF05_20000) - 4118834..4119811 (+) 978 WP_129324189.1 calcium/sodium antiporter -
  AAHF05_RS20035 (AAHF05_20005) kdsD 4119825..4120811 (+) 987 WP_129324190.1 arabinose-5-phosphate isomerase KdsD -
  AAHF05_RS20040 (AAHF05_20010) kdsC 4120826..4121392 (+) 567 WP_108091086.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  AAHF05_RS20045 (AAHF05_20015) lptC 4121389..4121964 (+) 576 WP_028017027.1 LPS export ABC transporter periplasmic protein LptC -
  AAHF05_RS20050 (AAHF05_20020) lptA 4121933..4122487 (+) 555 WP_024906352.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  AAHF05_RS20055 (AAHF05_20025) lptB 4122494..4123219 (+) 726 WP_025756692.1 LPS export ABC transporter ATP-binding protein -
  AAHF05_RS20060 (AAHF05_20030) rpoN/rpoN1 4123267..4124700 (+) 1434 WP_129324191.1 RNA polymerase factor sigma-54 Machinery gene
  AAHF05_RS20065 (AAHF05_20035) hpf 4124723..4125010 (+) 288 WP_008501444.1 ribosome hibernation promoting factor -
  AAHF05_RS20070 (AAHF05_20040) ptsN 4125093..4125584 (+) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AAHF05_RS20075 (AAHF05_20045) rapZ 4125630..4126484 (+) 855 WP_028017025.1 RNase adapter RapZ -
  AAHF05_RS20080 (AAHF05_20050) npr 4126481..4126753 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  AAHF05_RS20085 (AAHF05_20055) mtgA 4126793..4127518 (-) 726 WP_129324192.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  AAHF05_RS20090 (AAHF05_20060) elbB 4127515..4128168 (-) 654 WP_129324215.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54111.86 Da        Isoelectric Point: 4.3332

>NTDB_id=993046 AAHF05_RS20060 WP_129324191.1 4123267..4124700(+) (rpoN/rpoN1) [Enterobacter adelaidei strain ECC3473]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDDEIELEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWIDEARVIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=993046 AAHF05_RS20060 WP_129324191.1 4123267..4124700(+) (rpoN/rpoN1) [Enterobacter adelaidei strain ECC3473]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACCCAGCAGTCGCAGGATACAGAAGCGCTCGATACCGCTGATGCACTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTATCAGGGTGAAACCACCCAGTCGCTACAGGATTATCTGATGTGGCAGGTGGAACTGACCCCCTTCT
CCGATACCGACCGCGCGATTGCCACGTCAATTGTCGATGCCGTTGACGACACGGGCTATTTAACCGTCACGCTGGACGAT
ATTCTGGAAAGCATGGGCGACGACGAGATAGAGCTTGAGGAAGTTGAAGCCGTTCTGAAGCGTATCCAGCGTTTCGATCC
GGTCGGTGTGGCGGCAAAAGATCTACGCGACTGCCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCATGGATTG
ATGAGGCGCGCGTTATCATTAGCGATCATCTGGATCTGTTGGCTAACCACGATTTCCGCACCCTGATGCGCGTCACACGC
CTGAAAGAAGAGGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCGCTCGATCCACGCCCCGGACAGTCGATCCAGACCAG
CGAACCCGAGTACGTGATCCCGGATGTGCTGGTCAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAACTCTGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCACGAAACGACTCTGACAACCAATATATTCGT
AGCAATCTGCAGGAAGCACGATGGTTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTCCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCTTTCTTTGAGCAGGGTGAAGAGTATATGAAACCGATGGTACTGGCAGATATTGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGTCATGTGAATACCGAGGGCGGCGGTGAAGCGTCGTCAACGGCAATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTGAGTGACAGTAAGTTAACCACCATGCTGTCCGACCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment