Detailed information NTG0992576

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: MIDG2331_RS05245
• Genome accession: NZ_LN908249
• Coordinates: 1171966..1174182 (-)
• Length: 738 a.a.
• NCBI ID: WP_231944200.1
• Uniprot ID: -
• Organism: Actinobacillus pleuropneumoniae serovar 8 isolate MIDG2331
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1166966..1179182

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MIDG2331_RS05225 (MIDG2331_01068)	-	1168072..1168842 (-)	771	WP_005608235.1	glycosyltransferase family 25 protein	-
MIDG2331_RS05230 (MIDG2331_01069)	-	1168839..1170047 (-)	1209	WP_039755271.1	lipopolysaccharide biosynthesis protein	-
MIDG2331_RS05235 (MIDG2331_01070)	-	1170025..1170990 (-)	966	WP_005604751.1	polysaccharide pyruvyl transferase family protein	-
MIDG2331_RS05240 (MIDG2331_01071)	-	1171146..1171886 (+)	741	WP_005597819.1	YdcF family protein	-
MIDG2331_RS05245 (MIDG2331_01072)	priA	1171966..1174182 (-)	2217	WP_231944200.1	primosomal protein N'	Machinery gene
MIDG2331_RS05250 (MIDG2331_01073)	-	1174267..1175424 (-)	1158	WP_039709172.1	chorismate mutase	-
MIDG2331_RS05255 (MIDG2331_01074)	prlC	1175547..1177580 (-)	2034	WP_039709173.1	oligopeptidase A	-
MIDG2331_RS05260 (MIDG2331_01075)	pflA	1177723..1178460 (-)	738	WP_005597829.1	pyruvate formate lyase 1-activating protein	-

Sequence

Protein

• Length: 738 a.a.
• Molecular weight: 83201.96 Da
• Isoelectric Point: 8.8719

>NTDB_id=992576 MIDG2331_RS05245 WP_231944200.1 1171966..1174182(-) (priA) [Actinobacillus pleuropneumoniae serovar 8 isolate MIDG2331]
MSKLVRVALPVPLHRYFDYLLPPFLSAVKGVRVEVPFGNQTKIGVVVDFPETSDVPEDKLKPIKAVLDLEPIFDEEIWTL
LRWAARYYHAPIGEVAINALPVKLRNGDSTERSQPDYFAVTEAGRAALLSGENKRAKKQQDLLAKLAEIANFFEKPAACS
QSAWKGLLEKNYIELVNVPFEPQHWQKKLQNRPLVNLQNRLVLNKQQSLVVSRLTVQNGFEAFLLNGVTGSGKTEVYLQV
IEEVLKRGEQVLVLVPEIGLTPQTVQRFKARFNVEIDVLHSNMNETERLNAWLRAKNGESAIVIGTRSALFSQFEKLGLI
IIDEEHDSSFKQQDGWRYHARDLAILRAKNLHIPIILGSATPSLESVQNVQNGKFIELSLTARAGNAQQAKQQLIDLKTQ
RITAGLSDRLLAMMKRHLEQGNQVVLFLNRRGFAPVLLCHECGWMCECDACEKPYTYHQKQKILRCHHCASQRVIPRQCG
HCGSTNLITTGVGTEQLEQVLSEQFPTYQISRIDRDSTARKGALENHLNDIREGKSQILIGTQMLAKGHHFPNVTLVAIV
NVDSALFSTDFRAEERLAQLYVQVAGRAGRAEKQGEVVLQTHYPEHPLLKTLLEQGYLAFAQEALKMRKVMGLPPFAAQV
LLRATGKDNQQVVNLLQNLTAYFQQKCTELGLQDIQLLPPFSAPMAKKAGHYRWLLLIQHRSRGVLQQLIDQFDLDKANL
GLQNNIRLTLDIDPQEIG

Nucleotide

• Length: 2217 bp

>NTDB_id=992576 MIDG2331_RS05245 WP_231944200.1 1171966..1174182(-) (priA) [Actinobacillus pleuropneumoniae serovar 8 isolate MIDG2331]
ATGTCGAAGCTAGTTAGAGTGGCATTACCCGTGCCGTTACATCGTTATTTTGATTATTTACTGCCGCCTTTTTTGTCGGC
GGTCAAAGGCGTGCGTGTTGAAGTGCCTTTCGGTAATCAAACGAAAATCGGCGTGGTGGTGGATTTCCCTGAAACAAGCG
ATGTGCCTGAAGATAAGCTCAAACCGATTAAAGCGGTATTAGATTTAGAACCGATCTTTGATGAGGAAATTTGGACACTG
CTTCGTTGGGCGGCCCGTTATTATCATGCGCCTATCGGCGAAGTGGCGATCAATGCTTTGCCGGTGAAATTACGTAACGG
CGATAGCACCGAAAGATCGCAACCGGATTACTTTGCGGTTACTGAGGCAGGGCGAGCAGCATTGCTCTCGGGTGAGAACA
AGCGGGCAAAAAAACAGCAAGATTTACTGGCGAAACTGGCAGAAATTGCAAATTTTTTTGAAAAACCGGCCGCTTGTAGC
CAATCGGCTTGGAAAGGGCTATTGGAAAAAAACTATATCGAACTGGTTAATGTGCCGTTTGAACCGCAACATTGGCAAAA
AAAATTGCAAAATCGACCGCTTGTAAATTTGCAGAATCGACTGGTATTAAATAAACAGCAAAGCTTAGTGGTGAGCCGCT
TAACAGTACAGAACGGTTTCGAAGCTTTTTTATTAAATGGCGTAACCGGTTCAGGCAAAACCGAAGTGTATTTGCAAGTG
ATCGAAGAGGTGTTAAAGCGAGGCGAGCAAGTATTGGTGTTAGTACCGGAAATCGGGCTGACCCCGCAAACGGTGCAACG
TTTTAAAGCCCGTTTTAATGTTGAAATTGATGTTCTTCATTCGAATATGAATGAAACAGAGCGATTAAATGCTTGGTTGC
GAGCAAAAAACGGCGAAAGTGCGATTGTGATCGGCACGCGTTCGGCATTGTTCAGCCAGTTTGAGAAATTGGGCTTAATC
ATTATTGATGAAGAACACGATAGTTCTTTCAAACAACAAGACGGCTGGCGTTATCACGCAAGAGATTTGGCGATTTTACG
GGCGAAAAATTTACATATCCCGATTATTTTGGGTTCTGCAACGCCAAGTTTGGAAAGCGTACAAAACGTTCAAAACGGTA
AGTTTATTGAGCTATCTTTAACTGCTCGTGCCGGTAATGCTCAACAGGCGAAACAGCAATTGATTGATTTAAAAACACAG
CGTATTACCGCCGGACTTTCCGACCGCTTATTAGCGATGATGAAACGGCATTTGGAGCAAGGTAATCAGGTGGTGTTGTT
TTTAAATCGCCGTGGCTTTGCACCGGTTTTACTTTGTCACGAGTGTGGCTGGATGTGTGAATGTGACGCTTGTGAAAAGC
CTTACACTTATCATCAGAAACAGAAAATCTTGCGTTGTCATCATTGTGCTTCACAGCGAGTGATCCCTCGCCAGTGCGGG
CATTGCGGCTCAACCAATCTGATTACCACCGGAGTGGGGACGGAGCAGTTGGAGCAAGTGTTAAGTGAGCAATTCCCAAC
TTACCAAATTAGCCGTATCGACCGAGATTCCACCGCTCGTAAAGGGGCATTGGAAAACCACTTAAATGATATCCGAGAAG
GTAAAAGCCAAATCCTTATCGGCACGCAAATGTTAGCGAAAGGGCATCATTTTCCGAATGTAACGTTAGTGGCGATTGTG
AATGTAGATTCGGCACTGTTTTCGACCGACTTCCGTGCGGAAGAACGTTTGGCACAGCTTTATGTGCAAGTGGCGGGACG
TGCCGGACGAGCGGAAAAGCAGGGCGAAGTGGTATTACAAACTCATTATCCGGAACATCCGTTGCTCAAGACTTTGTTAG
AACAAGGTTATTTGGCGTTTGCACAAGAAGCGTTAAAAATGCGTAAAGTGATGGGCTTGCCGCCGTTTGCTGCGCAAGTG
TTGTTACGGGCAACCGGCAAAGATAATCAGCAAGTGGTAAATCTTTTGCAAAATTTGACCGCTTATTTTCAGCAAAAATG
TACGGAACTCGGTTTGCAGGATATCCAGCTTCTACCGCCTTTTTCCGCACCGATGGCTAAAAAAGCGGGGCATTACCGCT
GGCTTTTATTAATTCAACATCGCTCAAGAGGCGTATTACAACAGTTGATCGATCAATTTGATTTGGATAAAGCCAATTTA
GGCTTACAAAATAATATTCGTTTAACCTTAGATATTGATCCGCAAGAAATCGGCTAA

Domains

Predicted by InterProScan.

• DEAD/DEAH box helicase (DEAD) (221-369)

• Helicase conserved C-terminal domain (Helicase_C) (497-591)

• Primosomal protein N C-terminal domain (PriA_C) (635-734)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (6-101)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (448-473)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	43.414	100	0.438