Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   QMF30_RS02550 Genome accession   NZ_AP026974
Coordinates   468063..469427 (+) Length   454 a.a.
NCBI ID   WP_281745842.1    Uniprot ID   -
Organism   Polynucleobacter yangtzensis strain KF032     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 463063..474427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMF30_RS02530 (PKF032_04880) - 464877..465248 (+) 372 WP_281743173.1 glycine zipper family protein -
  QMF30_RS02540 (PKF032_04890) - 465580..467523 (+) 1944 WP_281745840.1 potassium transporter Kup -
  QMF30_RS02545 (PKF032_04900) - 467520..468029 (+) 510 WP_281745841.1 hypothetical protein -
  QMF30_RS02550 (PKF032_04910) radA 468063..469427 (+) 1365 WP_281745842.1 DNA repair protein RadA Machinery gene
  QMF30_RS02555 (PKF032_04920) prmB 469438..470334 (-) 897 WP_281745843.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase -
  QMF30_RS02560 (PKF032_04930) dapE 470378..471529 (-) 1152 WP_281745844.1 succinyl-diaminopimelate desuccinylase -
  QMF30_RS02565 (PKF032_04940) dapD 471539..472366 (-) 828 WP_068322474.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 48220.02 Da        Isoelectric Point: 7.3470

>NTDB_id=99176 QMF30_RS02550 WP_281745842.1 468063..469427(+) (radA) [Polynucleobacter yangtzensis strain KF032]
MAKVKTVYICQSCGGTSAKWQGQCPSCQAWNTLEEGLPETTSNARFQGLAQSLPRQKLSAITAEDLPRFSTGVEEFDRVL
GGGLVPGGVVLLGGDPGIGKSTLLLQALAEMSTAGMNVLYSSGEESAAQIALRAKRIALDAPQLEVLAEIQLEKLISIMD
TVKPQVLVVDSIQTLYSEVLSSAPGSVAQVRECAAQLTRAAKSSGICVLMVGHVTKDGHLAGPRVLEHIVDTVLYFEGDT
HSSFRLVRSIKNRFGAVNELGVFAMTEKGLRGVTNPSAIFLSQHEQMVPGACVLVTQEGSRPLLVEIQALVDTAHVPNPR
RLAVGLEQARLAMLLAVLHRHAGVACFDQDVFLNAVGGVKISEPAADLAVLLAIQSSIRNRALPKELIVFGEVGLAGEIR
PCPRGQERLKEAAKLGFTVAIIPKANMPKTKIPGLKVIPVERIDQAIAAAAELS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=99176 QMF30_RS02550 WP_281745842.1 468063..469427(+) (radA) [Polynucleobacter yangtzensis strain KF032]
TTGGCTAAAGTCAAAACGGTTTATATCTGTCAGTCCTGTGGTGGCACTTCTGCCAAGTGGCAAGGTCAGTGCCCATCTTG
TCAGGCCTGGAATACCTTGGAAGAAGGTCTTCCAGAAACCACCTCTAATGCTCGATTTCAGGGTTTGGCGCAATCCTTAC
CAAGACAAAAACTTTCTGCCATTACTGCGGAAGATTTGCCAAGATTTAGTACGGGTGTAGAAGAGTTTGATCGTGTGCTG
GGTGGCGGCTTAGTTCCAGGCGGCGTAGTACTCTTGGGTGGCGACCCTGGTATTGGTAAATCAACCTTACTATTGCAAGC
GCTTGCCGAGATGAGCACTGCTGGTATGAATGTGCTCTATAGCAGTGGTGAAGAGTCGGCCGCTCAGATTGCATTGCGTG
CAAAGCGTATTGCATTAGATGCTCCACAGTTGGAAGTCTTGGCAGAAATTCAGTTGGAGAAACTGATTTCCATTATGGAT
ACCGTGAAGCCACAAGTATTAGTGGTGGATTCCATTCAGACTTTGTACTCTGAGGTTTTGAGCTCAGCCCCTGGTTCTGT
TGCGCAAGTTCGTGAGTGTGCAGCGCAACTCACTCGAGCTGCAAAATCTAGTGGTATCTGTGTCTTAATGGTCGGTCACG
TTACCAAGGATGGTCATCTCGCTGGTCCTAGAGTTCTAGAGCATATCGTTGATACCGTTTTGTATTTCGAAGGTGATACC
CACTCATCTTTCCGTTTGGTGCGCTCCATTAAGAACCGTTTTGGCGCTGTCAATGAGTTGGGCGTATTTGCTATGACAGA
AAAGGGTTTGCGTGGCGTAACCAATCCATCTGCAATATTCTTGTCACAACACGAGCAAATGGTTCCTGGTGCTTGCGTGC
TGGTTACGCAAGAAGGCAGCAGACCTTTGTTGGTTGAGATTCAGGCTCTGGTGGATACGGCGCATGTGCCTAATCCTCGC
CGCTTGGCTGTTGGCTTGGAGCAGGCTCGTTTAGCAATGCTCTTGGCGGTATTGCATCGTCATGCAGGGGTTGCTTGCTT
TGACCAAGACGTATTTTTAAATGCGGTTGGTGGAGTTAAGATTTCTGAGCCGGCCGCTGACTTAGCGGTATTGCTTGCAA
TCCAGTCATCGATTCGTAATCGAGCATTGCCAAAAGAGTTGATTGTCTTTGGTGAGGTTGGTTTGGCGGGAGAGATTCGT
CCTTGCCCGCGCGGTCAGGAGCGTTTGAAGGAGGCTGCTAAATTGGGCTTCACTGTAGCTATCATTCCAAAAGCCAATAT
GCCTAAGACAAAGATTCCAGGATTGAAGGTCATTCCAGTTGAACGAATTGATCAGGCAATCGCAGCAGCAGCAGAGTTGA
GTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(78-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(357-450)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.336

99.559

0.491
  radA Streptococcus pneumoniae Rx1

46

99.119

0.456
  radA Streptococcus pneumoniae D39

46

99.119

0.456
  radA Streptococcus pneumoniae R6

46

99.119

0.456
  radA Streptococcus pneumoniae TIGR4

46

99.119

0.456
  radA Streptococcus mitis NCTC 12261

46

99.119

0.456
  radA Streptococcus mitis SK321

46

99.119

0.456

Multiple sequence alignment