Detailed information NTG0099164

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: PKF022_RS08930
• Genome accession: NZ_AP026972
• Coordinates: 1737770..1738660 (+)
• Length: 296 a.a.
• NCBI ID: WP_281776663.1
• Uniprot ID: -
• Organism: Polynucleobacter sp. KF022
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1732770..1743660

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PKF022_RS08900 (PKF022_17220)	pth	1732968..1733558 (+)	591	WP_281776658.1	aminoacyl-tRNA hydrolase	-
PKF022_RS08905 (PKF022_17230)	-	1733568..1733834 (-)	267	WP_281776659.1	YfhL family 4Fe-4S dicluster ferredoxin	-
PKF022_RS08910 (PKF022_17240)	coaD	1733852..1734349 (-)	498	WP_216230936.1	pantetheine-phosphate adenylyltransferase	-
PKF022_RS08915 (PKF022_17250)	rsmD	1734386..1734982 (-)	597	WP_281776660.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
PKF022_RS08920 (PKF022_17260)	-	1735005..1736342 (-)	1338	WP_281776661.1	pitrilysin family protein	-
PKF022_RS08925 (PKF022_17270)	-	1736339..1737607 (-)	1269	WP_281776662.1	pitrilysin family protein	-
PKF022_RS08930 (PKF022_17280)	pilA	1737770..1738660 (+)	891	WP_281776663.1	signal recognition particle-docking protein FtsY	Machinery gene
PKF022_RS08935 (PKF022_17290)	rpoH	1738753..1739676 (+)	924	WP_281776664.1	RNA polymerase sigma factor RpoH	-
PKF022_RS08940 (PKF022_17300)	-	1739713..1740297 (-)	585	WP_216230942.1	SCO family protein	-
PKF022_RS08945 (PKF022_17310)	cyoE	1740305..1741198 (-)	894	WP_216230943.1	heme o synthase	-
PKF022_RS08950 (PKF022_17320)	-	1741201..1742322 (-)	1122	WP_281776665.1	COX15/CtaA family protein	-
PKF022_RS08955 (PKF022_17330)	-	1742322..1742978 (-)	657	WP_281776666.1	hypothetical protein	-

Sequence

Protein

• Length: 296 a.a.
• Molecular weight: 31287.81 Da
• Isoelectric Point: 6.8448

>NTDB_id=99164 PKF022_RS08930 WP_281776663.1 1737770..1738660(+) (pilA) [Polynucleobacter sp. KF022]
MFGLRKTLGSLFKSSKVDESWFDTLEESLIQSDVGLPTTEQLISKLRKAAKSEKASSPEELQALLIQEVSTLLTAIEPSL
NPLFTGDKPNTPEVWLVVGVNGAGKTTTIGKLCRLFQSQGKSVLLAAGDTFRAAARNQLQEWGGRNQVDVITQEGGDAAA
VAHDAIHAAISRKSDILIIDTAGRLATQDHLMEELKKVKRVIGKALPGAPHHTLLVLDGNTGQNGLSQVKAFHAALGLTG
IIVTKLDGTAKGGVICALAHTLQEGPKPAVLALGKGEGIDDLAPFTAGQYSSELFN

Nucleotide

• Length: 891 bp

>NTDB_id=99164 PKF022_RS08930 WP_281776663.1 1737770..1738660(+) (pilA) [Polynucleobacter sp. KF022]
ATGTTCGGCTTACGTAAAACCCTAGGATCCCTTTTCAAATCAAGCAAGGTTGATGAATCTTGGTTTGATACTTTGGAAGA
ATCACTCATACAAAGTGATGTGGGTCTTCCTACGACCGAACAACTCATCAGCAAACTACGTAAAGCAGCAAAATCAGAGA
AGGCTTCCAGCCCCGAAGAGTTACAAGCGCTACTTATTCAAGAAGTCAGCACATTACTCACTGCTATTGAGCCTTCACTT
AATCCACTCTTTACTGGCGATAAGCCTAACACTCCAGAGGTATGGTTGGTTGTAGGGGTAAACGGTGCTGGCAAGACTAC
AACTATTGGCAAACTCTGCCGACTCTTTCAGTCTCAAGGTAAATCCGTTCTCCTGGCCGCAGGCGATACCTTCAGGGCTG
CTGCACGCAACCAACTTCAAGAATGGGGCGGACGCAATCAAGTGGATGTTATTACTCAAGAAGGTGGTGACGCTGCCGCG
GTTGCGCATGACGCGATTCATGCGGCCATCTCACGTAAGAGCGATATTCTGATCATTGATACAGCAGGCCGCTTAGCCAC
TCAAGACCATCTGATGGAAGAGCTCAAGAAGGTAAAGCGCGTCATCGGTAAAGCCCTTCCTGGGGCGCCACACCATACCC
TACTCGTCCTAGATGGCAACACAGGCCAAAATGGCTTAAGCCAAGTAAAAGCGTTTCATGCAGCCTTGGGGCTGACTGGA
ATCATCGTGACTAAGCTTGATGGCACCGCTAAAGGCGGCGTGATCTGCGCCCTTGCCCATACCCTACAAGAAGGCCCAAA
ACCCGCTGTTCTAGCCCTAGGCAAAGGTGAGGGAATTGATGATTTAGCCCCCTTCACAGCAGGGCAATATTCCTCTGAAT
TATTCAATTAA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (92-295)

• SRP54-type protein, helical bundle domain (SRP54_N) (4-68)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	52.881	99.662	0.527