Detailed information NTG0991247

Bioinformatically predicted

Overview

• Name: hexA
• Type: Machinery gene
• Locus tag: VV28_RS08950
• Genome accession: NZ_LN680001
• Coordinates: 1775720..1778296 (+)
• Length: 858 a.a.
• NCBI ID: WP_003244841.1
• Uniprot ID: P49849
• Organism: Bacillus subtilis strain BS34A
• Function: DNA mismatch repair (predicted from homology) Homologous recombination

Genomic Context

Location: 1770720..1783296

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VV28_RS08930 (BS34A_18670)	kbl	1771492..1772670 (+)	1179	WP_003231837.1	glycine C-acetyltransferase	-
VV28_RS08935 (BS34A_18680)	miaB	1772818..1774347 (+)	1530	WP_003244831.1	tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB	-
VV28_RS08940 (BS34A_18690)	ymcA	1774349..1774780 (+)	432	WP_003231834.1	regulatory iron-sulfur-containing complex subunit RicA	Regulator
VV28_RS08945 (BS34A_18700)	cotE	1775042..1775587 (+)	546	WP_003231833.1	outer spore coat protein CotE	-
VV28_RS08950 (BS34A_18710)	hexA	1775720..1778296 (+)	2577	WP_003244841.1	DNA mismatch repair protein MutS	Machinery gene
VV28_RS08955 (BS34A_18720)	hexB	1778312..1780195 (+)	1884	WP_003245099.1	DNA mismatch repair endonuclease MutL	Machinery gene
VV28_RS08960 (BS34A_18730)	ymzD	1780593..1781048 (-)	456	WP_003231830.1	regulatory YrvL family protein	-
VV28_RS08965 (BS34A_18750)	-	1781203..1781760 (-)	558	WP_003245720.1	hypothetical protein	-
VV28_RS08970 (BS34A_18760)	pksA	1781881..1782498 (+)	618	WP_003231827.1	TetR/AcrR family transcriptional regulator	-

Sequence

Protein

• Length: 858 a.a.
• Molecular weight: 97603.69 Da
• Isoelectric Point: 5.2488

>NTDB_id=991247 VV28_RS08950 WP_003244841.1 1775720..1778296(+) (hexA) [Bacillus subtilis strain BS34A]
MAGYTPMIQQYLKIKAEHQDAFLFFRLGDFYEMFFEDAKKASQELEITLTSRDGGAAEKIPMCGVPYHSASAYIEQLIKK
GYKVAICEQTEDPKAAKGVVKREVVQLITPGTVMDGKGIHESENNFIASVSACSNGYGLALSDLTTGENLAVLIERLEDV
ISEIYSVGAREIVVSGSLDADTVAQLRERCGATISIEDGETDEHVTIIEHLNNEDITKTFLRLYTYLKRTQKRSLDHLQP
VQVYELEEAMKIDLYSKRNLELTETIRSKNKKGSLLWLLDETKTAMGGRLLKQWIDRPLIRVNQIEERQEMVETLMSHFF
EREDLRERLKEVYDLERLAGRVAFGNVNARDLIQLKESLKQVPGIKQLVASLAHDKAKERAKRIDPCGDVLELLEEALYE
NPPLSVKEGNLIKDGYNQKLDEYRDASRNGKDWIARLEQQEREYTGIRSLKVGFNKVFGYYIEVTKANLHLLEEGRYERK
QTLTNAERYITPELKEKEALILEAENNICELEYELFTELREKVKQYIPRLQQLAKQMSELDALQCFATISENRHYTKPEF
SKDEVEVIEGRHPVVEKVMDSQEYVPNNCMMGDNRQMLLITGPNMSGKSTYMRQIALISIMAQIGCFVPAKKAVLPIFDQ
IFTRIGAADDLISGQSTFMVEMLEAKNAIVNATKNSLILFDEIGRGTSTYDGMALAQAIIEYVHDHIGAKTLFSTHYHEL
TVLEDKLPQLKNVHVRAEEYNGTVVFLHQIKEGAADKSYGIHVAQLAELPGDLIARAQDILKELEHSGNKPEVPVQKPQV
KEEPAQLSFFDEAEKPAETPKLSKKEKQVIDAFKSLNILDMTPLEAMNEMYKLQKKLH

Nucleotide

• Length: 2577 bp

>NTDB_id=991247 VV28_RS08950 WP_003244841.1 1775720..1778296(+) (hexA) [Bacillus subtilis strain BS34A]
ATGGCCGGTTATACGCCTATGATACAGCAATATTTAAAAATAAAGGCAGAGCACCAGGATGCCTTTTTATTTTTTCGCCT
GGGTGATTTTTATGAAATGTTTTTTGAGGACGCCAAAAAAGCGTCACAAGAGCTGGAAATTACGTTAACGAGCAGAGACG
GCGGTGCGGCTGAAAAAATACCGATGTGCGGTGTGCCGTATCATTCTGCTTCCGCGTATATCGAACAGCTTATTAAAAAA
GGATACAAAGTGGCGATCTGTGAACAGACGGAAGATCCGAAAGCCGCAAAGGGCGTTGTGAAAAGAGAAGTAGTTCAGCT
GATTACGCCCGGAACTGTAATGGACGGCAAAGGCATCCATGAGTCGGAAAATAATTTTATCGCATCTGTTTCAGCCTGCT
CGAACGGATACGGACTTGCTCTGTCTGATTTAACAACGGGAGAAAATTTGGCTGTTTTGATTGAACGGCTTGAAGACGTC
ATATCAGAAATTTATTCAGTCGGCGCACGGGAAATCGTGGTTTCAGGAAGCTTGGATGCCGATACAGTGGCACAGCTGAG
AGAGCGGTGCGGTGCAACGATTTCAATTGAAGACGGAGAAACAGACGAACACGTAACGATCATTGAGCACTTAAATAATG
AAGATATAACAAAAACATTTCTGCGCTTATATACGTATTTGAAAAGAACCCAAAAGCGCAGCCTTGATCATCTTCAGCCC
GTGCAGGTGTATGAGCTTGAGGAAGCGATGAAAATTGACTTGTACTCAAAGCGTAATCTGGAGCTGACCGAAACGATTCG
TTCGAAAAATAAAAAAGGCTCCCTTTTGTGGCTGCTGGATGAAACAAAAACGGCAATGGGAGGCCGGCTACTTAAACAGT
GGATTGACCGGCCGCTTATCAGAGTCAATCAAATCGAAGAGCGCCAAGAAATGGTGGAAACATTAATGTCCCATTTCTTT
GAACGGGAAGATCTGCGTGAACGTTTAAAAGAAGTATATGACTTAGAACGCCTTGCCGGCCGCGTTGCATTCGGAAATGT
CAATGCCCGGGATTTGATTCAGCTGAAGGAATCGTTAAAGCAAGTGCCTGGCATCAAACAGCTGGTTGCTTCACTGGCTC
ATGACAAGGCCAAGGAACGTGCAAAGCGCATTGACCCTTGCGGGGATGTACTTGAACTGCTGGAAGAAGCGCTGTACGAA
AACCCTCCTTTATCGGTAAAAGAAGGGAACCTGATTAAAGATGGTTATAATCAAAAGCTTGATGAATATCGTGATGCAAG
CAGAAACGGAAAAGACTGGATTGCCCGCCTGGAACAGCAGGAGCGGGAATACACAGGCATTCGCTCTTTAAAGGTCGGCT
TTAATAAAGTTTTCGGTTATTACATTGAAGTGACAAAAGCAAACTTGCATTTGCTTGAGGAAGGGCGTTATGAGCGGAAG
CAGACGTTAACCAATGCAGAGCGCTACATTACACCTGAATTAAAAGAAAAAGAAGCGCTCATTTTAGAGGCGGAAAATAA
CATCTGTGAGCTGGAGTACGAGCTGTTTACCGAGCTGCGCGAGAAAGTGAAGCAGTATATTCCGCGGCTGCAGCAGCTTG
CAAAACAGATGAGCGAGCTGGACGCACTGCAATGCTTTGCGACAATCAGTGAAAATCGCCACTACACGAAACCAGAGTTC
TCTAAAGATGAAGTCGAAGTGATTGAAGGCAGACACCCGGTTGTTGAAAAAGTCATGGACAGCCAGGAATATGTCCCGAA
CAACTGTATGATGGGCGATAACAGACAAATGCTTCTCATTACAGGTCCAAACATGTCAGGGAAAAGCACGTATATGAGAC
AAATCGCGCTCATTTCCATTATGGCGCAAATCGGCTGCTTTGTACCCGCGAAAAAAGCGGTGCTCCCGATTTTCGATCAA
ATTTTTACGCGAATCGGCGCTGCAGATGATTTGATTTCCGGACAAAGTACATTTATGGTAGAAATGCTTGAAGCGAAAAA
TGCGATTGTCAATGCGACGAAAAACAGCCTCATTCTGTTTGATGAAATCGGGCGGGGAACGTCCACTTATGACGGCATGG
CGCTGGCACAGGCGATTATTGAATATGTTCACGATCATATCGGCGCCAAGACGCTGTTCAGCACACACTATCACGAGCTG
ACCGTTCTTGAGGACAAGCTGCCCCAGCTGAAAAACGTTCATGTTCGCGCTGAAGAATATAACGGAACGGTTGTCTTTCT
TCATCAAATTAAAGAAGGTGCGGCTGATAAAAGCTATGGTATCCATGTAGCTCAGCTTGCCGAACTGCCGGGAGATCTGA
TTGCGCGCGCTCAAGATATTTTAAAAGAGCTTGAGCATTCAGGAAACAAACCGGAAGTGCCGGTGCAGAAACCTCAGGTG
AAAGAAGAGCCGGCACAGCTGTCCTTTTTTGACGAAGCGGAAAAGCCGGCTGAAACACCAAAGCTTTCAAAAAAAGAAAA
GCAAGTGATCGATGCTTTCAAATCACTTAATATACTGGATATGACACCGCTTGAAGCGATGAATGAAATGTACAAGCTGC
AAAAGAAATTACATTAA

Domains

Predicted by InterProScan.

• MutS domain V (MutS_V) (598-785)

• MutS family domain IV (MutS_IV) (415-506)

• MutS domain II (MutS_II) (125-239)

• MutS domain I (MutS_I) (5-116)

• MutS domain III (MutS_III) (258-547)

3D structure

• AlphaFold DB: P49849

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
hexA	Streptococcus pneumoniae R6	49.474	99.65	0.493
mutS	Pseudomonas stutzeri strain ATCC 17587	38.32	100	0.388