Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   ACNRMP_RS10310 Genome accession   NZ_JBLWDC010000001
Coordinates   2218726..2221032 (-) Length   768 a.a.
NCBI ID   WP_028853828.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain PD 7123     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2213726..2226032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNRMP_RS10305 (ACNRMP_10305) putA 2214587..2218564 (-) 3978 WP_028853827.1 trifunctional transcriptional regulator/proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase -
  ACNRMP_RS10310 (ACNRMP_10310) priA 2218726..2221032 (-) 2307 WP_028853828.1 primosomal protein N' Machinery gene
  ACNRMP_RS10315 (ACNRMP_10315) hemE 2221615..2222709 (-) 1095 WP_011003187.1 uroporphyrinogen decarboxylase -
  ACNRMP_RS10320 (ACNRMP_10320) - 2222851..2223321 (-) 471 WP_028853829.1 cupin domain-containing protein -
  ACNRMP_RS10325 (ACNRMP_10325) - 2223486..2223833 (+) 348 WP_011003189.1 GFA family protein -
  ACNRMP_RS10330 (ACNRMP_10330) - 2223862..2224242 (+) 381 WP_028853830.1 hypothetical protein -

Sequence

Protein


Download         Length: 768 a.a.        Molecular weight: 82880.38 Da        Isoelectric Point: 8.2063

>NTDB_id=990704 ACNRMP_RS10310 WP_028853828.1 2218726..2221032(-) (priA) [Ralstonia pseudosolanacearum strain PD 7123]
MTATRVDAAPPAVAQAARAGAIARVVIDTPLDAVFDYRCAQPVVPGQLVVVPFGSRRVVALVVETAGTTEVPEDRLRDVE
RVLDWVPPLDAEWRALAEFAAGYYHRALGEVTLPALPPLWRTPGSWDSLARHATETVYVMREPARAALLDSIPRRAAGAM
RLAEALTGDGELSASAAVALHGQATAKLRDWVAVGWVGAELRPRPLLPVPRDLPPASVAPALNAEQAAAVEAIAQAGAGG
GFAPFLLYGVTGSGKTEVYLHAIADALARDPAAQVLMLVPEINLTPQLEARIAARFPGVPLAALHSGLAEQPRALNWLAA
HRGEARIVLGTRLAMLASLPHLALILVDEEHDTSYKQQEGLRYSARDLALWRAKQRGIPVVLGSATPSLETWWRAEQGTT
ARLVLSQRAQADAVLPRVSLIDLTLERRAGREIRDGLSRPLLDAVAGRLARGEQSLLFLNRRGYAPVLACDACGWLSGCP
RCSAYLVLHKPEHRLRCHHCGYESRIPHHCPDCGNVDIAPLGRGTQRIEETLGALLPQARVARIDADSTRRKGSAEALFD
TVHEGAVDILIGTQMVAKGHDFQRVTLVGVVAPDASLFSHDFRAGEHLFASLMQVAGRAGRAGLAGEVLIQTRYPDAPAL
LALVRHDYAGFARQLLRERKQACLPPFAYQALLTAEHRELARALEFLGAARAAGQAVAEAQGLPVQLHDPVPMTMVRLAN
RERAQLLLESASRAALQRLLATWTDGFGDIGRTVKGVRWQLEIDPLRI

Nucleotide


Download         Length: 2307 bp        

>NTDB_id=990704 ACNRMP_RS10310 WP_028853828.1 2218726..2221032(-) (priA) [Ralstonia pseudosolanacearum strain PD 7123]
ATGACCGCTACCCGTGTTGATGCCGCTCCGCCCGCCGTTGCCCAGGCAGCGCGCGCGGGCGCCATTGCGCGGGTGGTGAT
CGATACGCCGCTGGATGCCGTATTCGACTATCGCTGCGCGCAGCCGGTCGTGCCGGGCCAGCTGGTGGTGGTGCCGTTCG
GCAGCCGGCGCGTGGTGGCGCTGGTGGTGGAGACCGCCGGCACCACCGAGGTGCCGGAAGACCGCCTGCGCGATGTCGAG
CGCGTGCTCGACTGGGTGCCGCCGCTGGATGCCGAATGGCGCGCGCTGGCCGAATTCGCCGCGGGCTACTACCACCGGGC
GCTGGGTGAAGTGACGCTGCCGGCGCTGCCGCCGTTGTGGCGTACGCCCGGCAGCTGGGACAGCCTGGCCCGCCATGCCA
CCGAGACCGTCTACGTGATGCGCGAGCCGGCGCGCGCCGCCTTGCTCGACAGCATCCCCCGGCGGGCGGCCGGGGCGATG
CGCCTGGCCGAAGCGCTGACGGGCGACGGCGAACTGTCCGCCAGCGCCGCTGTCGCGCTGCACGGGCAGGCGACGGCCAA
GCTGCGCGATTGGGTCGCGGTGGGCTGGGTCGGGGCGGAGCTGCGTCCCAGGCCGTTGCTGCCGGTGCCGCGCGATCTGC
CGCCGGCGTCCGTCGCGCCGGCGCTCAATGCCGAGCAGGCTGCTGCCGTCGAAGCCATCGCGCAGGCCGGGGCGGGCGGC
GGTTTCGCGCCGTTCCTGCTGTACGGGGTCACCGGCAGCGGCAAGACCGAGGTCTACCTGCACGCCATCGCCGATGCCCT
CGCGCGCGATCCGGCCGCGCAGGTGCTGATGCTGGTGCCCGAGATCAACCTGACGCCGCAGCTGGAGGCGCGCATTGCGG
CGCGCTTCCCCGGCGTGCCGCTGGCCGCGCTGCACAGCGGCCTGGCCGAGCAGCCGCGCGCGCTGAACTGGCTGGCGGCG
CATCGCGGCGAGGCGCGCATCGTGCTCGGCACGCGGCTGGCGATGCTGGCCTCGCTGCCGCACCTCGCGCTGATCCTCGT
CGACGAGGAGCACGACACATCCTATAAGCAGCAAGAGGGCCTGCGCTATTCCGCGCGCGATCTGGCGCTGTGGCGGGCCA
AGCAGCGCGGCATCCCGGTCGTGCTCGGGTCGGCCACGCCGTCGCTGGAGACCTGGTGGCGCGCCGAGCAGGGCACCACC
GCGCGCCTGGTGCTGTCGCAGCGCGCGCAGGCCGATGCGGTGCTGCCGCGCGTCTCGCTGATCGACCTGACGCTGGAGCG
CCGCGCGGGCCGGGAGATCCGCGACGGGCTGTCCAGGCCGCTGCTCGATGCCGTTGCCGGGCGCCTGGCGCGCGGCGAGC
AGAGCCTGCTGTTCCTCAACCGCCGTGGCTACGCGCCGGTGCTGGCGTGCGACGCCTGCGGCTGGCTGTCGGGCTGCCCG
CGCTGCTCGGCGTACCTGGTGCTGCACAAGCCGGAGCACCGGCTGCGCTGCCACCACTGCGGCTACGAATCGCGCATCCC
GCACCATTGCCCGGACTGCGGCAACGTTGACATCGCGCCGCTGGGGCGCGGCACGCAGCGCATCGAGGAGACCCTCGGCG
CGCTGCTTCCGCAGGCGCGCGTGGCGCGCATCGACGCCGACTCCACCCGCCGCAAGGGCAGCGCCGAGGCGCTGTTCGAC
ACCGTGCACGAAGGGGCCGTCGATATCCTGATCGGCACGCAGATGGTCGCCAAGGGGCATGACTTCCAGCGCGTGACGCT
GGTGGGCGTGGTGGCGCCCGATGCGTCCCTGTTCAGCCACGACTTCCGTGCCGGCGAGCACCTGTTCGCCTCGCTGATGC
AGGTGGCCGGGCGCGCCGGCCGCGCCGGGCTGGCGGGCGAAGTGCTGATCCAGACCCGCTACCCGGATGCCCCCGCCCTG
CTGGCGCTGGTGCGGCACGATTACGCCGGCTTCGCGCGCCAGCTGCTGCGCGAGCGCAAGCAGGCCTGCCTGCCGCCGTT
TGCCTACCAGGCGCTGCTGACCGCCGAGCACCGCGAGTTGGCGCGCGCGCTGGAATTTCTCGGCGCCGCGCGCGCGGCCG
GGCAGGCCGTGGCCGAAGCCCAGGGCCTGCCGGTGCAACTGCACGATCCGGTGCCCATGACCATGGTGCGCCTGGCCAAC
CGCGAACGCGCCCAACTGCTGCTCGAAAGCGCTTCGCGCGCCGCCCTGCAGCGGCTGCTGGCCACCTGGACCGACGGGTT
TGGCGACATCGGCCGGACGGTCAAGGGCGTGCGCTGGCAGCTCGAGATCGACCCGCTGCGGATCTGA

Domains

Predicted by InterProScan.

3DNA-binding domain (3BD) (PriA_3primeBD)

(24-117)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(479-505)

Primosomal protein N C-terminal domain (PriA_C)

(666-765)

DEAD/DEAH box helicase (DEAD)

(226-388)

Helicase conserved C-terminal domain (Helicase_C)

(540-623)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

39.333

97.656

0.384