Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   O2W21_RS17140 Genome accession   NZ_JAPVBX010000001
Coordinates   3546367..3547749 (+) Length   460 a.a.
NCBI ID   WP_001029698.1    Uniprot ID   A0A2T3RLH6
Organism   Escherichia coli strain KE21     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3541367..3552749
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O2W21_RS17115 (O2W21_17080) deoD 3542381..3543100 (+) 720 WP_000224877.1 purine-nucleoside phosphorylase -
  O2W21_RS17120 (O2W21_17085) - 3543261..3543524 (-) 264 WP_001281310.1 helix-turn-helix transcriptional regulator -
  O2W21_RS17125 (O2W21_17090) lplA 3543556..3544572 (-) 1017 WP_000105849.1 lipoate--protein ligase LplA -
  O2W21_RS17130 (O2W21_17095) ytjB 3544600..3545244 (-) 645 WP_000124615.1 YtjB family periplasmic protein -
  O2W21_RS17135 (O2W21_17100) serB 3545350..3546318 (+) 969 WP_001132956.1 phosphoserine phosphatase -
  O2W21_RS17140 (O2W21_17105) radA/sms 3546367..3547749 (+) 1383 WP_001029698.1 DNA repair protein RadA Machinery gene
  O2W21_RS17145 (O2W21_17110) nadR 3547770..3549002 (+) 1233 WP_000093813.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  O2W21_RS25360 nadS 3549036..3549164 (+) 129 Protein_3349 NadS family protein -
  O2W21_RS17155 (O2W21_17120) - 3549243..3549791 (-) 549 WP_000576894.1 GNAT family N-acetyltransferase -
  O2W21_RS17160 (O2W21_17125) - 3549875..3550054 (-) 180 WP_001407311.1 hypothetical protein -
  O2W21_RS17165 (O2W21_17130) - 3550014..3550130 (-) 117 Protein_3352 hypothetical protein -
  O2W21_RS17170 (O2W21_17135) - 3550203..3550415 (-) 213 WP_000848792.1 DUF1471 domain-containing protein -
  O2W21_RS17175 (O2W21_17140) - 3550573..3550989 (-) 417 WP_086118697.1 SRPBCC family protein -
  O2W21_RS17180 (O2W21_17145) - 3551015..3551812 (-) 798 WP_000762716.1 SDR family NAD(P)-dependent oxidoreductase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49442.02 Da        Isoelectric Point: 7.1936

>NTDB_id=990619 O2W21_RS17140 WP_001029698.1 3546367..3547749(+) (radA/sms) [Escherichia coli strain KE21]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=990619 O2W21_RS17140 WP_001029698.1 3546367..3547749(+) (radA/sms) [Escherichia coli strain KE21]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGTGGCT
ATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGA
TTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCGGGAAGCGCCATTCTGATTGGCGGTAACCCAGGTGCGGG
GAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACTGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAA
CAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAACTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGT
ACAATCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGCGTGG
CGATTGTCATGGTCGGGCACGTAACCAAAGATGGCTCGCTGGCTGGCCCTAAAGTGCTGGAACACTGTATTGACTGTTCG
GTGCTTCTGGACGGCGATGCCGACTCCCGTTTCCGCACCTTGCGTAGCCATAAAAACCGCTTCGGCGCGGTGAATGAACT
GGGCGTCTTCGCGATGACCGAACAGGGTCTGCGTGAAGTCAGCAACCCTTCGGCAATCTTTTTAAGCCGCGGTGATGAAG
TGACCTCCGGTAGCTCAGTGATGGTAGTGTGGGAAGGAACGCGTCCGCTGCTGGTGGAGATCCAGGCGCTGGTCGATCAC
TCGATGATGGCGAATCCACGCCGCGTGGCGGTAGGGCTGGAGCAAAACCGTCTGGCGATCCTGCTGGCTGTCTTGCACCG
TCACGGTGGTCTGCAAATGGCCGATCAGGATGTATTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCG
ACTTGGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGTATCTCAGAAGCGGCGAAACACGGTTTTCGCCGGGC
GATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3RLH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.827

99.348

0.465
  radA Streptococcus mitis SK321

43.86

99.13

0.435
  radA Streptococcus pneumoniae D39

43.421

99.13

0.43
  radA Streptococcus pneumoniae TIGR4

43.421

99.13

0.43
  radA Streptococcus mitis NCTC 12261

43.421

99.13

0.43
  radA Streptococcus pneumoniae R6

43.421

99.13

0.43
  radA Streptococcus pneumoniae Rx1

43.421

99.13

0.43