Detailed information NTG0990475

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAHY48_RS21505
• Genome accession: NZ_CP153131
• Coordinates: 4429146..4429946 (+)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola subsp. variicola strain T7-472
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4424146..4434946

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAHY48_RS21470 (AAHY48_21440)	gspH	4424299..4424814 (+)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-
AAHY48_RS21475 (AAHY48_21445)	gspI	4424811..4425176 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
AAHY48_RS21480 (AAHY48_21450)	gspJ	4425173..4425769 (+)	597	WP_239270627.1	type II secretion system minor pseudopilin GspJ	-
AAHY48_RS21485 (AAHY48_21455)	gspK	4425766..4426746 (+)	981	WP_016160312.1	type II secretion system minor pseudopilin GspK	-
AAHY48_RS21490 (AAHY48_21460)	gspL	4426739..4427935 (+)	1197	WP_032732533.1	type II secretion system protein GspL	-
AAHY48_RS21495 (AAHY48_21465)	-	4427928..4428413 (+)	486	WP_012968921.1	type II secretion system protein M	-
AAHY48_RS21500 (AAHY48_21470)	-	4428403..4429149 (+)	747	WP_016160310.1	type II secretion system protein N	-
AAHY48_RS21505 (AAHY48_21475)	pilD	4429146..4429946 (+)	801	WP_008807384.1	A24 family peptidase	Machinery gene
AAHY48_RS21510 (AAHY48_21480)	hrpB	4429969..4432398 (-)	2430	WP_239270626.1	ATP-dependent helicase HrpB	-
AAHY48_RS21515 (AAHY48_21485)	thpR	4432471..4433007 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
AAHY48_RS21520 (AAHY48_21490)	sfsA	4433007..4433723 (+)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
AAHY48_RS21525 (AAHY48_21495)	dksA	4433886..4434341 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=990475 AAHY48_RS21505 WP_008807384.1 4429146..4429946(+) (pilD) [Klebsiella variicola subsp. variicola strain T7-472]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=990475 AAHY48_RS21505 WP_008807384.1 4429146..4429946(+) (pilD) [Klebsiella variicola subsp. variicola strain T7-472]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425