Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MWH27_RS13345 Genome accession   NZ_JALJVV010000001
Coordinates   2468656..2469066 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain A-5     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2463656..2474066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWH27_RS13315 (MWH27_13290) - 2463744..2463884 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  MWH27_RS13320 (MWH27_13295) yqeF 2464190..2464921 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  MWH27_RS13325 (MWH27_13300) cwlH 2465173..2465925 (-) 753 WP_014480318.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  MWH27_RS13330 (MWH27_13305) yqeD 2466112..2466738 (+) 627 WP_014480319.1 TVP38/TMEM64 family protein -
  MWH27_RS13335 (MWH27_13310) gnd 2466757..2467650 (-) 894 WP_014480320.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  MWH27_RS13340 (MWH27_13315) yqeB 2467901..2468623 (+) 723 WP_014480321.1 hypothetical protein -
  MWH27_RS13345 (MWH27_13320) nucA/comI 2468656..2469066 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  MWH27_RS13350 (MWH27_13325) sigK 2469262..2469990 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  MWH27_RS13355 (MWH27_13330) - 2469990..2470088 (+) 99 WP_031600702.1 hypothetical protein -
  MWH27_RS13360 (MWH27_13335) - 2470085..2470297 (-) 213 Protein_2580 recombinase family protein -
  MWH27_RS13365 (MWH27_13340) fumC 2470516..2471904 (-) 1389 WP_014480325.1 class II fumarate hydratase -
  MWH27_RS13370 (MWH27_13345) - 2472071..2472961 (+) 891 WP_014480326.1 LysR family transcriptional regulator -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=990412 MWH27_RS13345 WP_009967785.1 2468656..2469066(-) (nucA/comI) [Bacillus subtilis strain A-5]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=990412 MWH27_RS13345 WP_009967785.1 2468656..2469066(-) (nucA/comI) [Bacillus subtilis strain A-5]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529